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-isoforms of AMPK
during and after exercise. Healthy human subjects performed bicycle exercise on two
separate occasions at either a low (~50% maximum rate of O2 uptake
(VO2,max) for 90 min) or a high (~75% VO2,max
for 60 min) intensity. Biopsies from the vastus lateralis muscle were obtained before
and immediately after exercise, and after 3 h of recovery.
2-AMPK isoform immediately after
high intensity exercise, whereas no activation was observed after low intensity exercise.
The activation of 2-AMPK was totally reversed 3 h after exercise. In contrast, 1-AMPK
was not activated during either of the two exercise trials.
2-AMPK was significantly greater than
that of 1-AMPK (~3- vs. ~2-fold).
2-AMPK during exercise is dependent
upon exercise intensity. The stable activation of 2-AMPK, presumably due to the
activation of an upstream AMPK kinase, is compatible with a role for this kinase
complex in the regulation of skeletal muscle metabolism during exercise, whereas
the lack of stable 1-AMPK activation makes this kinase complex a less likely candidate.
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N. Musi, M. F. |
