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J Physiol Volume 530, Number 1, 47-54, January 1, 2001
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Journal of Physiology (2001), 530.1, pp. 47-54
© Copyright 2001 The Physiological Society

17beta-Oestradiol acutely regulates Cl- secretion in rat distal colonic epithelium


Steven B. Condliffe, Christina M. Doolan and Brian J. Harvey


Wellcome Trust Cellular Physiology Research Unit, Physiology Department, University College Cork, Ireland

  1. In this study we used the short circuit current (ISC) technique to measure the non-genomic effects of the female sex steroid 17beta-oestradiol (E2) on electrogenic transepithelial ion transport in rat distal colonic epithelium.

  2. Basal ISC was largely composed of a transepithelial Cl- secretory component with minimal electrogenic Na+ movement. E2 (1-100 nM) caused a significant decrease in basal ISC after 15 min. In addition, pre-treating colonic epithelial tissues with E2 (0.1-100 nM) for 10 min significantly reduced forskolin (20 muM)-induced Cl- secretion. E2 also down-regulated Cl- secretion which was pre-stimulated by forskolin. Cl- secretory responses to the Ca2+-dependent secretagogue carbachol (10 muM) were also significantly reduced in the presence of E2 (10- 100 nM). However, E2 had no effect on amiloride-sensitive Na+ absorption.

  3. The rapid anti-secretory effect of E2 was abolished in the presence of the intracellular Ca2+ chelator BAPTA (50 muM) or the protein kinase C (PKC) inhibitor chelerythrine chloride (1 muM). However, in the presence of the nuclear oestrogen receptor antagonist tamoxifen (10 muM), E2 still produced an inhibition of Cl- secretion. Testosterone, progesterone and 17alpha-oestradiol had no significant effect on colonic Cl- secretion. Also, E2 (100 nM) did not alter Cl- secretion in colonic epithelia isolated from male rats.

  4. We conclude that E2 inhibits colonic Cl- secretion via a non-genomic pathway that involves intracellular Ca2+ and PKC. It is possible that this gender-specific mechanism contributes to the salt and water retention associated with high E2 states.




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