HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pan, Z. Articles by McKinnon, D. Search for Related Content PubMed PubMed Citation Articles by Pan, Z. Articles by McKinnon, D.

Alternative splicing of KCNQ2 potassium channel transcripts contributes to the functional diversity of M-currents

Zongming Pan, Alexander A. Selyanko*, Jennifer K. Hadley*, David A. Brown*, Jane E. Dixon† and David McKinnon

1. The region of alternative splicing in the KCNQ2 potassium channel gene was determined by RNase protection analysis of KCNQ2 mRNA transcripts.

2. Systematic analysis of KCNQ2 alternative splice variant expression in rat superior cervical ganglia revealed multiple variant isoforms.

3. One class of KCNQ2 splice variants, those that contained exon 15a, was found to have significantly different kinetics to those of the other isoforms. These transcripts encoded channel subunits that, when co-expressed with the KCNQ3 subunit, activated and deactivated approximately 2·5 times more slowly than other isoforms. Deletion of exon 15a in these isoforms produced a reversion to the faster kinetics.

4. Comparison of the kinetic properties of the cloned channel splice variants with those of the native M-current suggests that alternative splicing of the KCNQ2 gene may contribute to the variation in M-current kinetics seen in vivo.

This article has been cited by other articles:

				C. L. Wladyka and D. L. Kunze KCNQ/M-currents contribute to the resting membrane potential in rat visceral sensory neurons J. Physiol., August 15, 2006; 575(1): 175 - 189. [Abstract] [Full Text] [PDF]

				J. R. Schwarz, G. Glassmeier, E. C. Cooper, T.-C Kao, H. Nodera, D. Tabuena, R. Kaji, and H. Bostock KCNQ channels mediate IKs, a slow K+ current regulating excitability in the rat node of Ranvier J. Physiol., May 15, 2006; 573(1): 17 - 34. [Abstract] [Full Text] [PDF]

				Z. Pan, T. Kao, Z. Horvath, J. Lemos, J.-Y. Sul, S. D. Cranstoun, V. Bennett, S. S. Scherer, and E. C. Cooper A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. J. Neurosci., March 8, 2006; 26(10): 2599 - 2613. [Abstract] [Full Text] [PDF]

				W. Shen, S. E. Hamilton, N. M. Nathanson, and D. J. Surmeier Cholinergic Suppression of KCNQ Channel Currents Enhances Excitability of Striatal Medium Spiny Neurons J. Neurosci., August 10, 2005; 25(32): 7449 - 7458. [Abstract] [Full Text] [PDF]

				D. L. Prole and N. V. Marrion Ionic Permeation and Conduction Properties of Neuronal KCNQ2/KCNQ3 Potassium Channels Biophys. J., March 1, 2004; 86(3): 1454 - 1469. [Abstract] [Full Text] [PDF]

				J. J. Devaux, K. A. Kleopa, E. C. Cooper, and S. S. Scherer KCNQ2 Is a Nodal K+ Channel J. Neurosci., February 4, 2004; 24(5): 1236 - 1244. [Abstract] [Full Text] [PDF]

				D. L. Prole, P. A. Lima, and N. V. Marrion Mechanisms Underlying Modulation of Neuronal KCNQ2/KCNQ3 Potassium Channels by Extracellular Protons J. Gen. Physiol., November 24, 2003; 122(6): 775 - 793. [Abstract] [Full Text] [PDF]

				G. M. Passmore, A. A. Selyanko, M. Mistry, M. Al-Qatari, S. J. Marsh, E. A. Matthews, A. H. Dickenson, T. A. Brown, S. A. Burbidge, M. Main, et al. KCNQ/M Currents in Sensory Neurons: Significance for Pain Therapy J. Neurosci., August 6, 2003; 23(18): 7227 - 7236. [Abstract] [Full Text] [PDF]

				J. K. Hadley, G. M. Passmore, L. Tatulian, M. Al-Qatari, F. Ye, A. D. Wickenden, and D. A. Brown Stoichiometry of Expressed KCNQ2/KCNQ3 Potassium Channels and Subunit Composition of Native Ganglionic M Channels Deduced from Block by Tetraethylammonium J. Neurosci., June 15, 2003; 23(12): 5012 - 5019. [Abstract] [Full Text] [PDF]

				S. Ohya, G. P. Sergeant, I. A. Greenwood, and B. Horowitz Molecular Variants of KCNQ Channels Expressed in Murine Portal Vein Myocytes: A Role in Delayed Rectifier Current Circ. Res., May 16, 2003; 92(9): 1016 - 1023. [Abstract] [Full Text] [PDF]

				H. Wen and I. B. Levitan Calmodulin Is an Auxiliary Subunit of KCNQ2/3 Potassium Channels J. Neurosci., September 15, 2002; 22(18): 7991 - 8001. [Abstract] [Full Text] [PDF]

				A. Ozaita, M. E. Martone, M. H. Ellisman, and B. Rudy Differential Subcellular Localization of the Two Alternatively Spliced Isoforms of the Kv3.1 Potassium Channel Subunit in Brain J Neurophysiol, July 1, 2002; 88(1): 394 - 408. [Abstract] [Full Text] [PDF]

				E. C. Cooper, E. Harrington, Y. N. Jan, and L. Y. Jan M Channel KCNQ2 Subunits Are Localized to Key Sites for Control of Neuronal Network Oscillations and Synchronization in Mouse Brain J. Neurosci., December 15, 2001; 21(24): 9529 - 9540. [Abstract] [Full Text] [PDF]

				W. Gu, G. Schlichthorl, J. R. Hirsch, H. Engels, C. Karschin, A. Karschin, C. Derst, O. K. Steinlein, and J. Daut Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2 J. Physiol., March 15, 2002; 539(3): 657 - 668. [Abstract] [Full Text] [PDF]