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J Physiol Volume 533, Number 3, 711-716, June 15, 2001
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Journal of Physiology (2001), 533.3, pp. 711-716
© Copyright 2001 The Physiological Society

GABA-evoked chloride currents do not differ between dendrites and somata of rat neocortical neurons


Johannes F. M. van Brederode *†, Tomoko Takigawa ‡ and Christian Alzheimer ‡


* Puget Sound VA Health Center, Seattle, WA 98108, USA, † Department of Neurology , University of Washington, School of Medicine, Seattle, WA 98195, USA and ‡ Department of Physiology, University of Munich, D-80336 Munich, Germany

  1. We performed patch-clamp recordings on acutely isolated somata and dendritic segments of rat neocortical neurons, in order to compare the reversal potential (EGABA) and relative density of GABAA receptor-mediated Cl- currents in these two cellular compartments.
  2. Currents were recorded with the Cl--impermeable pore former gramicidin (25-75 µg ml-1) in HCO3--free bath solution. Voltage ramps (-110 to -30 mV) from a holding potential (Vh) of -60 mV in the absence and presence of 2 µM GABA were used to construct instantaneous current-voltage relationships. Currents were abolished by co-application of GABA with the GABAA receptor antagonist bicuculline (40 µM).
  3. GABA conductance, normalized to membrane surface area, was not different in somata and dendrites. In addition, EGABA was not different in the two compartments.
  4. Replacement of intracellular K+ with Cs+ resulted in a significantly more depolarized EGABA in both somata and dendrites. These results suggest that the resting intracellular Cl- concentration ([Cl-]i) is similar in somata and dendrites and that an outward Cl- transporter system maintains low [Cl-]i.



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