HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wretman, C. Articles by Henriksson, J. Search for Related Content PubMed PubMed Citation Articles by Wretman, C. Articles by Henriksson, J.

Effects of concentric and eccentric contractions on phosphorylation of MAPK^erk1/2 and MAPK^p38 in isolated rat skeletal muscle

Charlott Wretman, Arimantas Lionikas, Ulrika Widegren, Jan Lännergren, Håkan Westerblad and Jan Henriksson

1. Exercise and contractions of isolated skeletal muscle induce phosphorylation of mitogen-activated protein kinases (MAPKs) by undefined mechanisms. The aim of the present study was to determine exercise-related triggering factors for the increased phosphorylation of MAPKs in isolated rat extensor digitorum longus (EDL) muscle.
2. Concentric or eccentric contractions, or mild or severe passive stretches were used to discriminate between effects of metabolic/ionic and mechanical alterations on phosphorylation of two MAPKs: extracellular signal-regulated kinase 1 and 2 (MAPK^erk1/2) and stress-activated protein kinase p38 (MAPK^p38).
3. Concentric contractions induced a 5-fold increase in MAPK^erk1/2 phosphorylation. Application of the antioxidants N-acetylcysteine (20 mM) or dithiothreitol (5 mM) suppressed concentric contraction-induced increase in MAPK^erk1/2 phosphorylation. Mild passive stretches of the muscle increased MAPK^erk1/2 phosphorylation by 1.8-fold, whereas the combination of acidosis and passive stretches resulted in a 2.8-fold increase. Neither concentric contractions, nor mild stretches nor acidosis significantly affected phosphorylation of MAPK^p38.
4. High force applied upon muscle by means of either eccentric contractions or severe passive stretches resulted in 5.7- and 9.5-fold increases of phosphorylated MAPK^erk1/2, respectively, whereas phosphorylation of MAPK^p38 increased by 7.6- and 1.9-fold (not significant), respectively.
5. We conclude that in isolated rat skeletal muscle an increase in phosphorylation of both MAPK^erk1/2 and MAPK^p38 is induced by mechanical alterations, whereas contraction-related metabolic/ionic changes (reactive oxygen species and acidosis) cause increased phosphorylation of MAPK^erk1/2 only. Thus, contraction-induced phosphorylation can be explained by the combined action of increased production of reactive oxygen species, acidification and mechanical perturbations for MAPK^erk1/2 and by high mechanical stress for MAPK^p38.

This article has been cited by other articles:

				C. M. Stary, B. J. Walsh, A. E. Knapp, D. Brafman, and M. C. Hogan Elevation in heat shock protein 72 mRNA following contractions in isolated single skeletal muscle fibers Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R642 - R648. [Abstract] [Full Text] [PDF]

				H. F. Kramer and L. J. Goodyear Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle J Appl Physiol, July 1, 2007; 103(1): 388 - 395. [Abstract] [Full Text] [PDF]

				M. J. Jackson, D. Pye, and J. Palomero The production of reactive oxygen and nitrogen species by skeletal muscle J Appl Physiol, April 1, 2007; 102(4): 1664 - 1670. [Abstract] [Full Text] [PDF]

				C. A. Shaw, N. Larochelle, R. W.R. Dudley, H. Lochmuller, G. Danialou, B. J. Petrof, G. Karpati, P. C. Holland, and J. Nalbantoglu Simultaneous Dystrophin and Dysferlin Deficiencies Associated with High-Level Expression of the Coxsackie and Adenovirus Receptor in Transgenic Mice Am. J. Pathol., December 1, 2006; 169(6): 2148 - 2160. [Abstract] [Full Text] [PDF]

				D. W. Russ and R. M. Lovering Influence of activation frequency on cellular signalling pathways during fatiguing contractions in rat skeletal muscle Exp Physiol, November 1, 2006; 91(6): 957 - 966. [Abstract] [Full Text] [PDF]

				J. T. Lanner, A. Katz, P. Tavi, M. E. Sandstrom, S.-J. Zhang, C. Wretman, S. James, J. Fauconnier, J. Lannergren, J. D. Bruton, et al. The role of Ca2+ influx for insulin-mediated glucose uptake in skeletal muscle. Diabetes, July 1, 2006; 55(7): 2077 - 2083. [Abstract] [Full Text] [PDF]

				K. J. Nadeau, L. B. Ehlers, L. E. Aguirre, R. L. Moore, K. N. Jew, H. K. Ortmeyer, B. C. Hansen, J. E. B. Reusch, and B. Draznin Exercise training and calorie restriction increase SREBP-1 expression and intramuscular triglyceride in skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E90 - E98. [Abstract] [Full Text] [PDF]

				K. Stathopoulou, C. Gaitanaki, and I. Beis Extracellular pH changes activate the p38-MAPK signalling pathway in the amphibian heart J. Exp. Biol., April 1, 2006; 209(7): 1344 - 1354. [Abstract] [Full Text] [PDF]

				P. R. Chess, M. A. O'Reilly, F. Sachs, and J. N. Finkelstein Reactive oxidant and p42/44 MAP kinase signaling is necessary for mechanical strain-induced proliferation in pulmonary epithelial cells J Appl Physiol, September 1, 2005; 99(3): 1226 - 1232. [Abstract] [Full Text] [PDF]

				N. C. Jones, K. J. Tyner, L. Nibarger, H. M. Stanley, D. D.W. Cornelison, Y. V. Fedorov, and B. B. Olwin The p38{alpha}/{beta} MAPK functions as a molecular switch to activate the quiescent satellite cell J. Cell Biol., April 11, 2005; 169(1): 105 - 116. [Abstract] [Full Text] [PDF]

				J. N. Dentel, S. G. Blanchard, D. P. Ankrapp, L. R. McCabe, and R. W. Wiseman Inhibition of cross-bridge formation has no effect on contraction-associated phosphorylation of p38 MAPK in mouse skeletal muscle Am J Physiol Cell Physiol, April 1, 2005; 288(4): C824 - C830. [Abstract] [Full Text] [PDF]

				H. K. R. Karlsson, P.-A. Nilsson, J. Nilsson, A. V. Chibalin, J. R. Zierath, and E. Blomstrand Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E1 - E7. [Abstract] [Full Text] [PDF]

				M. KJAeR Role of Extracellular Matrix in Adaptation of Tendon and Skeletal Muscle to Mechanical Loading Physiol Rev, April 1, 2004; 84(2): 649 - 698. [Abstract] [Full Text] [PDF]

				I. A. Barash, L. Mathew, A. F. Ryan, J. Chen, and R. L. Lieber Rapid muscle-specific gene expression changes after a single bout of eccentric contractions in the mouse Am J Physiol Cell Physiol, February 1, 2004; 286(2): C355 - C364. [Abstract] [Full Text]

				Y.-W. Chen, M. J. Hubal, E. P. Hoffman, P. D. Thompson, and P. M. Clarkson Molecular responses of human muscle to eccentric exercise J Appl Physiol, December 1, 2003; 95(6): 2485 - 2494. [Abstract] [Full Text]

				P. D. Thompson, P. Clarkson, and R. H. Karas Statin-Associated Myopathy JAMA, April 2, 2003; 289(13): 1681 - 1690. [Abstract] [Full Text] [PDF]

				A. Kumar, I. Chaudhry, M. B. Reid, and A. M. Boriek Distinct Signaling Pathways Are Activated in Response to Mechanical Stress Applied Axially and Transversely to Skeletal Muscle Fibers J. Biol. Chem., November 22, 2002; 277(48): 46493 - 46503. [Abstract] [Full Text] [PDF]

				K. Sakamoto and L. J. Goodyear Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle J Appl Physiol, July 1, 2002; 93(1): 369 - 383. [Abstract] [Full Text] [PDF]