HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roth, A. Articles by Häusser, M. Search for Related Content PubMed PubMed Citation Articles by Roth, A. Articles by Häusser, M.

Compartmental models of rat cerebellar Purkinje cells based on simultaneous somatic and dendritic patch-clamp recordings

Arnd Roth * and Michael Häusser †‡

1. Simultaneous dendritic and somatic patch-clamp recordings were made from Purkinje cells in cerebellar slices from 12- to 21-day-old rats. Voltage responses to current impulses injected via either the dendritic or the somatic pipette were obtained in the presence of the selective I_h blocker ZD 7288 and blockers of spontaneous synaptic input. Neurons were filled with biocytin for subsequent morphological reconstruction.
2. Four neurons were reconstructed and converted into detailed compartmental models. The specific membrane capacitance (C_m), specific membrane resistance (R_m) and intracellular resistivity (R_i) were optimized by direct fitting of the model responses to the electrophysiological data from the same cell. Mean values were: C_m, 0.77 ± 0.17 µF cm^-2 (mean ± S.D.; range, 0.64-1.00 µF cm^-2), R_m, 122 ± 18 k cm² (98-141 k cm²) and R_i, 115 ± 20 cm (93-142 cm).
3. The steady-state electrotonic architecture of these cells was compact under the experimental conditions used. However, somatic voltage-clamp recordings of parallel fibre and climbing fibre synaptic currents were substantially filtered and attenuated.
4. The detailed models were compared with a two-compartment model of Purkinje cells. The range of synaptic current kinetics that can be faithfully recorded using somatic voltage clamp is predicted fairly well by the two-compartment model, even though some of its underlying assumptions are violated.
5. A model of I_h was constructed based on voltage-clamp data, and inserted into the passive compartmental models. Somatic EPSP amplitude was substantially attenuated compared to the amplitude of dendritic EPSPs at their site of generation. However, synaptic efficacy of the same quantal synaptic conductance, as measured by the somatic EPSP amplitude, was only weakly dependent on synaptic location on spiny branchlets.
6. The passive electrotonic structure of Purkinje cells is unusual in that the steady-state architecture is very compact, while voltage transients such as synaptic potentials and action potentials are heavily filtered.

This article has been cited by other articles:

				Q. Wen and D. B. Chklovskii A Cost-Benefit Analysis of Neuronal Morphology J Neurophysiol, May 1, 2008; 99(5): 2320 - 2328. [Abstract] [Full Text] [PDF]

				P. J. Sjostrom, E. A. Rancz, A. Roth, and M. Hausser Dendritic Excitability and Synaptic Plasticity Physiol Rev, April 1, 2008; 88(2): 769 - 840. [Abstract] [Full Text] [PDF]

				M. J. E. Richardson and G. Silberberg Measurement and Analysis of Postsynaptic Potentials Using a Novel Voltage-Deconvolution Method J Neurophysiol, February 1, 2008; 99(2): 1020 - 1031. [Abstract] [Full Text] [PDF]

				D. V. Sarkisov and S. S.-H. Wang Order-Dependent Coincidence Detection in Cerebellar Purkinje Neurons at the Inositol Trisphosphate Receptor J. Neurosci., January 2, 2008; 28(1): 133 - 142. [Abstract] [Full Text] [PDF]

				L. Sarid, R. Bruno, B. Sakmann, I. Segev, and D. Feldmeyer Modeling a layer 4-to-layer 2/3 module of a single column in rat neocortex: Interweaving in vitro and in vivo experimental observations PNAS, October 9, 2007; 104(41): 16353 - 16358. [Abstract] [Full Text] [PDF]

				K. Angelo, M. London, S. R. Christensen, and M. Hausser Local and Global Effects of Ih Distribution in Dendrites of Mammalian Neurons J. Neurosci., August 8, 2007; 27(32): 8643 - 8653. [Abstract] [Full Text] [PDF]

				C. Schmidt-Hieber, P. Jonas, and J. Bischofberger Subthreshold Dendritic Signal Processing and Coincidence Detection in Dentate Gyrus Granule Cells J. Neurosci., August 1, 2007; 27(31): 8430 - 8441. [Abstract] [Full Text] [PDF]

				J. M. Bekkers and M. Hausser Targeted dendrotomy reveals active and passive contributions of the dendritic tree to synaptic integration and neuronal output PNAS, July 3, 2007; 104(27): 11447 - 11452. [Abstract] [Full Text] [PDF]

				F. R. Fernandez, J. D. T. Engbers, and R. W. Turner Firing Dynamics of Cerebellar Purkinje Cells J Neurophysiol, July 1, 2007; 98(1): 278 - 294. [Abstract] [Full Text] [PDF]

				W. Mittmann and M. Hausser Linking Synaptic Plasticity and Spike Output at Excitatory and Inhibitory Synapses onto Cerebellar Purkinje Cells J. Neurosci., May 23, 2007; 27(21): 5559 - 5570. [Abstract] [Full Text] [PDF]

				A. T. Schaefer, M. Helmstaedter, A. C. Schmitt, D. Bar-Yehuda, M. Almog, H. Ben-Porat, B. Sakmann, and A. Korngreen Dendritic voltage-gated K+ conductance gradient in pyramidal neurones of neocortical layer 5B from rats J. Physiol., March 15, 2007; 579(3): 737 - 752. [Abstract] [Full Text] [PDF]

				S. P. Peron, H. G. Krapp, and F. Gabbiani Influence of Electrotonic Structure and Synaptic Mapping on the Receptive Field Properties of a Collision-Detecting Neuron J Neurophysiol, January 1, 2007; 97(1): 159 - 177. [Abstract] [Full Text] [PDF]

				M.-C. Perreault and M. Raastad Contribution of morphology and membrane resistance to integration of fast synaptic signals in two thalamic cell types J. Physiol., November 15, 2006; 577(1): 205 - 220. [Abstract] [Full Text] [PDF]

				Q. J. M. Huys, M. B. Ahrens, and L. Paninski Efficient Estimation of Detailed Single-Neuron Models J Neurophysiol, August 1, 2006; 96(2): 872 - 890. [Abstract] [Full Text] [PDF]

				E. Hartveit and M. L. Veruki Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous synaptic currents J. Physiol., August 1, 2006; 574(3): 751 - 785. [Abstract] [Full Text] [PDF]

				R. Brette and W. Gerstner Adaptive Exponential Integrate-and-Fire Model as an Effective Description of Neuronal Activity J Neurophysiol, November 1, 2005; 94(5): 3637 - 3642. [Abstract] [Full Text] [PDF]

				B. E McKay and R. W Turner Physiological and morphological development of the rat cerebellar Purkinje cell J. Physiol., September 15, 2005; 567(3): 829 - 850. [Abstract] [Full Text] [PDF]

				R. E. Sims and N. A. Hartell Differences in Transmission Properties and Susceptibility to Long-Term Depression Reveal Functional Specialization of Ascending Axon and Parallel Fiber Synapses to Purkinje Cells J. Neurosci., March 23, 2005; 25(12): 3246 - 3257. [Abstract] [Full Text] [PDF]

				F. Santamaria and J. M. Bower Background Synaptic Activity Modulates the Response of a Modeled Purkinje Cell to Paired Afferent Input J Neurophysiol, January 1, 2005; 93(1): 237 - 250. [Abstract] [Full Text] [PDF]

				P. Isope and T. H. Murphy Low threshold calcium currents in rat cerebellar Purkinje cell dendritic spines are mediated by T-type calcium channels J. Physiol., January 1, 2005; 562(1): 257 - 269. [Abstract] [Full Text] [PDF]

				B. W Edmonds, F. D Gregory, and F. E Schweizer Evidence that fast exocytosis can be predominantly mediated by vesicles not docked at active zones in frog saccular hair cells J. Physiol., October 15, 2004; 560(2): 439 - 450. [Abstract] [Full Text] [PDF]

				M. Canepari, C. Auger, and D. Ogden Ca2+ Ion Permeability and Single-Channel Properties of the Metabotropic Slow EPSC of Rat Purkinje Neurons J. Neurosci., April 7, 2004; 24(14): 3563 - 3573. [Abstract] [Full Text] [PDF]

				P. Isope, R. Franconville, B. Barbour, and P. Ascher Repetitive firing of rat cerebellar parallel fibres after a single stimulation J. Physiol., February 1, 2004; 554(3): 829 - 839. [Abstract] [Full Text] [PDF]

				T. V. Bui, S. Cushing, D. Dewey, R. E. Fyffe, and P. K. Rose Comparison of the Morphological and Electrotonic Properties of Renshaw Cells, Ia Inhibitory Interneurons, and Motoneurons in the Cat J Neurophysiol, November 1, 2003; 90(5): 2900 - 2918. [Abstract] [Full Text] [PDF]

				S. Hallermann, C. Pawlu, P. Jonas, and M. Heckmann A large pool of releasable vesicles in a cortical glutamatergic synapse PNAS, July 22, 2003; 100(15): 8975 - 8980. [Abstract] [Full Text] [PDF]

				M. Martina, G. L. Yao, and B. P. Bean Properties and Functional Role of Voltage-Dependent Potassium Channels in Dendrites of Rat Cerebellar Purkinje Neurons J. Neurosci., July 2, 2003; 23(13): 5698 - 5707. [Abstract] [Full Text] [PDF]

				A. T. Schaefer, M. E. Larkum, B. Sakmann, and A. Roth Coincidence Detection in Pyramidal Neurons Is Tuned by Their Dendritic Branching Pattern J Neurophysiol, June 1, 2003; 89(6): 3143 - 3154. [Abstract] [Full Text] [PDF]

				A. T. Schaefer, M. Helmstaedter, B. Sakmann, and A. Korngreen Correction of Conductance Measurements in Non-Space-Clamped Structures: 1. Voltage-Gated K+ Channels Biophys. J., June 1, 2003; 84(6): 3508 - 3528. [Abstract] [Full Text] [PDF]

				H. Schmidt, E. B. Brown, B. Schwaller, and J. Eilers Diffusional Mobility of Parvalbumin in Spiny Dendrites of Cerebellar Purkinje Neurons Quantified by Fluorescence Recovery after Photobleaching Biophys. J., April 1, 2003; 84(4): 2599 - 2608. [Abstract] [Full Text] [PDF]

				C.-C. Lien and P. Jonas Kv3 Potassium Conductance is Necessary and Kinetically Optimized for High-Frequency Action Potential Generation in Hippocampal Interneurons J. Neurosci., March 15, 2003; 23(6): 2058 - 2068. [Abstract] [Full Text] [PDF]

				M. Womack and K. Khodakhah Active Contribution of Dendrites to the Tonic and Trimodal Patterns of Activity in Cerebellar Purkinje Neurons J. Neurosci., December 15, 2002; 22(24): 10603 - 10612. [Abstract] [Full Text] [PDF]

				P. Isope and B. Barbour Properties of Unitary Granule Cellright-arrowPurkinje Cell Synapses in Adult Rat Cerebellar Slices J. Neurosci., November 15, 2002; 22(22): 9668 - 9678. [Abstract] [Full Text] [PDF]

				S. R. Williams, S. R. Christensen, G. J. Stuart, and M. Hausser Membrane potential bistability is controlled by the hyperpolarization-activated current IH in rat cerebellar Purkinje neurons in vitro J. Physiol., March 1, 2002; 539(2): 469 - 483. [Abstract] [Full Text] [PDF]