| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In vascular endothelial cells, elevation of cytosolic free calcium concentration ([Ca2+]i) causes activation of nitric oxide synthase (NOS) and release of nitric oxide (NO). The goal of the study was to characterize the interplay between [Ca2+]i and NO production in this cell type. Simultaneous measurements of [Ca2+]i and intracellular NO concentration ([NO]i) in cultured bovine vascular endothelial cells (CPAE cell line) with the fluorescent indicators fura-2 and DAF-2, respectively, revealed that Ca2+ influx following agonist-induced intracellular Ca2+ store depletion (capacitative Ca2+ entry, CCE) represents the preferential Ca2+ source for the activation of the Ca2+-calmodulin-dependent endothelial NOS (eNOS). Exposure to the NO donor sodium nitroprusside (SNP) showed that high NO levels suppressed CCE and had an inhibitory effect on Ca2+ extrusion by the plasmalemmal Ca2+-ATPase. This inhibitory effect on CCE was mimicked by the membrane-permeant cGMP analogue 8-bromo-cGMP, but was reversed by the NO scavenger haemoglobin and prevented by the inhibitor of the NO-sensitive guanylate cyclase ODQ. Brief exposure to SNP reduced the peak of ATP-induced Ca2+ release from the endoplasmic reticulum (ER) and accelerated Ca2+ reuptake into the ER. Prolonged incubation with SNP resulted in enhanced Ca2+ loading of the ER, as revealed by direct measurements of store content with the ER-entrapped low-affinity Ca2+ indicator mag-fura-2. The results suggest that in vascular endothelial cells, NO synthesis is under autoregulatory control that involves NO-dependent [Ca2+]i regulation. Via cGMP-dependent inhibition of CCE and acceleration of Ca2+ sequestration into the ER, NO can lower [Ca2+]i and therefore exert an autoregulatory negative feedback on its own Ca2+-dependent synthesis.
This article has been cited by other articles:
|
|
 |
|
  M. Foller, S. Feil, K. Ghoreschi, S. Koka, A. Gerling, M. Thunemann, F. Hofmann, B. Schuler, J. Vogel, B. Pichler, et al. Anemia and splenomegaly in cGKI-deficient mice PNAS, May 6, 2008; 105(18): 6771 - 6776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yin, J. Hoffmann, S. M. Kaestle, N. Neye, L. Wang, J. Baeurle, W. Liedtke, S. Wu, H. Kuppe, A. R. Pries, et al. Negative-Feedback Loop Attenuates Hydrostatic Lung Edema via a cGMP-Dependent Regulation of Transient Receptor Potential Vanilloid 4 Circ. Res., April 25, 2008; 102(8): 966 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, J. L. Pluznick, D. C. Settles, and S. C. Sansom Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1768 - F1776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Aromolaran, A. V. Zima, and L. A. Blatter Role of glycolytically generated ATP for CaMKII-mediated regulation of intracellular Ca2+ signaling in bovine vascular endothelial cells Am J Physiol Cell Physiol, July 1, 2007; 293(1): C106 - C118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Z. Sheng and A. P. Braun Small- and intermediate-conductance Ca2+-activated K+ channels directly control agonist-evoked nitric oxide synthesis in human vascular endothelial cells Am J Physiol Cell Physiol, July 1, 2007; 293(1): C458 - C467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. N. Dedkova, Y. G. Wang, X. Ji, L. A. Blatter, A. M. Samarel, and S. L. Lipsius Signalling mechanisms in contraction-mediated stimulation of intracellular NO production in cat ventricular myocytes J. Physiol., April 1, 2007; 580(1): 327 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Dawson, D. C. Zawieja, M. H. Wu, and H. J. Granger Signaling pathways mediating VEGF165-induced calcium transients and membrane depolarization in human endothelial cells FASEB J, May 1, 2006; 20(7): 991 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mewe, C. K. Bauer, D. Muller, and R. Middendorff Regulation of Spontaneous Contractile Activity in the Bovine Epididymal Duct by Cyclic Guanosine 5'-Monophosphate-Dependent Pathways Endocrinology, April 1, 2006; 147(4): 2051 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Jernigan, B. R. S. Broughton, B. R. Walker, and T. C. Resta Impaired NO-dependent inhibition of store- and receptor-operated calcium entry in pulmonary vascular smooth muscle after chronic hypoxia Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L517 - L525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. S. Aromolaran and L. A. Blatter Modulation of intracellular Ca2+ release and capacitative Ca2+ entry by CaMKII inhibitors in bovine vascular endothelial cells Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1426 - C1436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Davidson and D. M. Yellon The role of nitric oxide in mitochondria. Focus on "Modulation of mitochondrial Ca2+ by nitric oxide in cultured bovine vascular endothelial cells" Am J Physiol Cell Physiol, October 1, 2005; 289(4): C775 - C777. [Full Text] [PDF]
|
|
|
|
|
|
E. N. Dedkova and L. A. Blatter Modulation of mitochondrial Ca2+ by nitric oxide in cultured bovine vascular endothelial cells Am J Physiol Cell Physiol, October 1, 2005; 289(4): C836 - C845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. McNally, A. Saxena, H. Cai, S. Dikalov, and D. G. Harrison Regulation of Xanthine Oxidoreductase Protein Expression by Hydrogen Peroxide and Calcium Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1623 - 1628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Parekh and J. W. Putney Jr. Store-Operated Calcium Channels Physiol Rev, April 1, 2005; 85(2): 757 - 810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. NAVARRO, B. ANAND-APTE, and M.-O. PARAT A role for caveolae in cell migration FASEB J, December 1, 2004; 18(15): 1801 - 1811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takeuchi, H. Watanabe, Q.-K. Tran, M. Ozeki, D. Sumi, T. Hayashi, A. Iguchi, L. J Ignarro, K. Ohashi, and H. Hayashi Nitric oxide: inhibitory effects on endothelial cell calcium signaling, prostaglandin I2 production and nitric oxide synthase expression Cardiovasc Res, April 1, 2004; 62(1): 194 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Kwan, Y. Huang, and X. Yao Regulation of canonical transient receptor potential isoform 3 (TRPC3) channel by protein kinase G PNAS, February 24, 2004; 101(8): 2625 - 2630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. N. Dedkova, X. Ji, S. L. Lipsius, and L. A. Blatter Mitochondrial calcium uptake stimulates nitric oxide production in mitochondria of bovine vascular endothelial cells Am J Physiol Cell Physiol, February 1, 2004; 286(2): C406 - C415. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. M. Kuebler, U. Uhlig, T. Goldmann, G. Schael, A. Kerem, K. Exner, C. Martin, E. Vollmer, and S. Uhlig Stretch Activates Nitric Oxide Production in Pulmonary Vascular Endothelial Cells In Situ Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1391 - 1398. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
