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This Article Full Text Full Text (PDF) All Versions of this Article: 539/2/409    most recent 2001.012943v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yin, Y. Articles by Lemos, J. R. Search for Related Content PubMed PubMed Citation Articles by Yin, Y. Articles by Lemos, J. R.

Ca²⁺-regulated, neurosecretory granule channel involved in release from neurohypophysial terminals

Yong Yin, Govindan Dayanithi* and José R. Lemos

Ion channels from bovine neurohypophysial secretory granules (NSG) were incorporated into artificial lipid bilayers. Specific antibodies against identified synaptic vesicle proteins were tested on such incorporated channel activity and on peptide release from rat permeabilized neurohypophysial terminals. Both the NSG cation channel and Ca²⁺-dependent release were inhibited by only SY-38, a monoclonal antibody directed against the C-terminus of synaptophysin. SY-38 and Ca²⁺ altered both the gating and conductance of the NSG cation channel, but in opposite ways. The close correlation between SY-38 effects on Ca²⁺-dependent channel activity and release leads us to conclude that this synaptophysin-like NSG channel is directly involved in peptide secretion from these central nervous system terminals.

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				D. Gincel and V. Shoshan-Barmatz The Synaptic Vesicle Protein Synaptophysin: Purification and Characterization of Its Channel Activity Biophys. J., December 1, 2002; 83(6): 3223 - 3229. [Abstract] [Full Text] [PDF]