| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle of humans. To determine whether pre-exercise muscle glycogen content influences the magnitude and/or duration of this adaptive response, six male subjects performed one-legged cycling exercise to lower muscle glycogen content in one leg and then, the following day, completed 2.5 h low intensity two-legged cycling exercise. Nuclei and mRNA were isolated from biopsies obtained from the vastus lateralis muscle of the control and reduced glycogen (pre-exercise glycogen = 609 ± 47 and 337 ± 33 mmol kg-1 dry weight, respectively) legs before and after 0, 2 and 5 h of recovery. Exercise induced a significant (P < 0.05) increase (2- to 3-fold) in transcription of the pyruvate dehydrogenase kinase 4 (PDK4) and uncoupling protein 3 (UCP3) genes in the reduced glycogen leg only. Although PDK4, lipoprotein lipase (LPL) and hexokinase II (HKII) mRNA were elevated in the reduced glycogen leg before exercise, no consistent difference was found between the two legs in response to exercise. In a second study, six subjects completed two trials (separated by 2 weeks) consisting of 3 h of two-legged knee extensor exercise with either control (398 ± 52 mmol kg-1 dry weight) or low (240 ± 38 mmol kg-1 dry weight) pre-exercise muscle glycogen. Exercise induced a significantly greater increase in PDK4 transcription in the low glycogen (> 6-fold) than in the control (< 3-fold) trial. Induction of PDK4 and UCP3 mRNA in response to exercise was also signficantly higher in the low glycogen (11.4- and 3.5-fold, respectively) than in the control (5.0- and 1.7-fold, respectively) trial. These data indicate that low muscle glycogen content enhances the transcriptional activation of some metabolic genes in response to exercise, raising the possibility that signalling mechanisms sensitive to glycogen content and/or FFA availability may be linked to the transcriptional control of exercise-responsive genes.
This article has been cited by other articles:
|
|
 |
|
  K. De Bock, W. Derave, B. O. Eijnde, M. K. Hesselink, E. Koninckx, A. J. Rose, P. Schrauwen, A. Bonen, E. A. Richter, and P. Hespel Effect of training in the fasted state on metabolic responses during exercise with carbohydrate intake J Appl Physiol, April 1, 2008; 104(4): 1045 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Trappe, A. Creer, K. Minchev, D. Slivka, E. Louis, N. Luden, and T. Trappe Human soleus single muscle fiber function with exercise or nutrition countermeasures during 60 days of bed rest Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R939 - R947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gleeson Immune function in sport and exercise J Appl Physiol, August 1, 2007; 103(2): 693 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Churchley, V. G. Coffey, D. J. Pedersen, A. Shield, K. A. Carey, D. Cameron-Smith, and J. A. Hawley Influence of preexercise muscle glycogen content on transcriptional activity of metabolic and myogenic genes in well-trained humans J Appl Physiol, April 1, 2007; 102(4): 1604 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Pilegaard, J. B. Birk, M. Sacchetti, M. Mourtzakis, D. G. Hardie, G. Stewart, P. D. Neufer, B. Saltin, G. van Hall, and J. F.P. Wojtaszewski PDH-E1{alpha} Dephosphorylation and Activation in Human Skeletal Muscle During Exercise: Effect of Intralipid Infusion Diabetes, November 1, 2006; 55(11): 3020 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Greenberg, M. J. Jurczak, A. M. Danos, and M. J. Brady Glycogen branches out: new perspectives on the role of glycogen metabolism in the integration of metabolic pathways Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E1 - E8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mourtzakis, B. Saltin, T. Graham, and H. Pilegaard Carbohydrate metabolism during prolonged exercise and recovery: interactions between pyruvate dehydrogenase, fatty acids, and amino acids J Appl Physiol, June 1, 2006; 100(6): 1822 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Cluberton, S. L. McGee, R. M. Murphy, and M. Hargreaves Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression J Appl Physiol, October 1, 2005; 99(4): 1359 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bruunsgaard Physical activity and modulation of systemic low-level inflammation J. Leukoc. Biol., October 1, 2005; 78(4): 819 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Creer, P. Gallagher, D. Slivka, B. Jemiolo, W. Fink, and S. Trappe Influence of muscle glycogen availability on ERK1/2 and Akt signaling after resistance exercise in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 950 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, T. E. Graham, D. S. Battram, D. L. Robinson, E. A. Richter, R. J. Wilson, and M. Bakovic Glycogenin activity and mRNA expression in response to volitional exhaustion in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 957 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, R. J. Wilson, D. S. Battram, E. A. Richter, D. L. Robinson, M. Bakovic, and T. E. Graham Increases in glycogenin and glycogenin mRNA accompany glycogen resynthesis in human skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E508 - E514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yang, A. Creer, B. Jemiolo, and S. Trappe Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle J Appl Physiol, May 1, 2005; 98(5): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Hansen, C. P. Fischer, P. Plomgaard, J. L. Andersen, B. Saltin, and B. K. Pedersen Skeletal muscle adaptation: training twice every second day vs. training once daily J Appl Physiol, January 1, 2005; 98(1): 93 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Fischer, P. Plomgaard, A. K. Hansen, H. Pilegaard, B. Saltin, and B. K. Pedersen Endurance training reduces the contraction-induced interleukin-6 mRNA expression in human skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1189 - E1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Coles, J. Litt, H. Hatta, and A. Bonen Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle J. Physiol., November 15, 2004; 561(1): 253 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M J Watt, R J Southgate, A G Holmes, and M A Febbraio Suppression of plasma free fatty acids upregulates peroxisome proliferator-activated receptor (PPAR) {alpha} and {delta} and PPAR coactivator 1{alpha} in human skeletal muscle, but not lipid regulatory genes J. Mol. Endocrinol., October 1, 2004; 33(2): 533 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, G. J. F. Heigenhauser, P. J. LeBlanc, J. G. Inglis, L. L. Spriet, and S. J. Peters Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exercise J Appl Physiol, October 1, 2004; 97(4): 1261 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jensen, H. Pilegaard, P. D. Neufer, and Y. Hellsten Effect of acute exercise and exercise training on VEGF splice variants in human skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R397 - R402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Arkinstall, R. J. Tunstall, D. Cameron-Smith, and J. A. Hawley Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E25 - E31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Spriet, R. J. Tunstall, M. J. Watt, K. A. Mehan, M. Hargreaves, and D. Cameron-Smith Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fasting J Appl Physiol, June 1, 2004; 96(6): 2082 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kristensen and T. Hansen Statistical analyses of repeated measures in physiological research: a tutorial Advan Physiol Educ, March 1, 2004; 28(1): 2 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Terada and I. Tabata Effects of acute bouts of running and swimming exercise on PGC-1{alpha} protein expression in rat epitrochlearis and soleus muscle Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E208 - E216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Chakravarthy and F. W. Booth Eating, exercise, and "thrifty" genotypes: connecting the dots toward an evolutionary understanding of modern chronic diseases J Appl Physiol, January 1, 2004; 96(1): 3 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Hildebrandt, H. Pilegaard, and P. D. Neufer Differential transcriptional activation of select metabolic genes in response to variations in exercise intensity and duration Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1021 - E1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nordsborg, J. Bangsbo, and H. Pilegaard Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism J Appl Physiol, September 1, 2003; 95(3): 1201 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. C. Hesselink, P. Schrauwen, J. O. Holloszy, and T. E. Jones Divergent effects of acute exercise and endurance training on UCP3 expression Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E449 - E451. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
