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This Article Full Text Full Text (PDF) All Versions of this Article: 542/1/181    most recent 2001.013298v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geukes Foppen, R. J. Articles by van Heukelom, J. S. Search for Related Content PubMed PubMed Citation Articles by Geukes Foppen, R. J. Articles by van Heukelom, J. S.

Effects of chloride transport on bistable behaviour of the membrane potential in mouse skeletal muscle

R. J. Geukes Foppen, H. G. J. van Mil * and J. Siegenbeek van Heukelom

The lumbrical skeletal muscle fibres of mice exhibited electrically bistable behaviour due to the nonlinear properties of the inwardly rectifying potassium conductance. When the membrane potential (V_m) was measured continuously using intracellular microelectrodes, either a depolarization or a hyperpolarization was observed following reduction of the extracellular potassium concentration (K) from 5.7 mM to values in the range 0.76-3.8 mM, and V_m showed hysteresis when K was slowly decreased and then increased within this range. Hypertonicity caused membrane depolarization by enhancing chloride import through the Na⁺-K⁺-2Cl^- cotransporter and altered the bistable behaviour of the muscle fibres. Addition of bumetanide, a potent inhibitor of the Na⁺-K⁺-2Cl^- cotransporter, and of anthracene-9-carboxylic acid, a blocker of chloride channels, caused membrane hyperpolarization particularly under hypertonic conditions, and also altered the bistable behaviour of the cells. Hysteresis loops shifted with hypertonicity to higher K values and with bumetanide to lower values. The addition of 80 µM BaCl₂ or temperature reduction from 35 to 27 °C induced a depolarization of cells that were originally hyperpolarized. In the K range of 5.7-22.8 mM, cells in isotonic media (289 mmol kg^-1) responded nearly Nernstianly to K reduction, i.e. 50 mV per decade; in hypertonic media this dependence was reduced to 36 mV per decade (319 mmol kg^-1) or to 31 mV per decade (340 mmol kg^-1). Our data can explain apparent discrepancies in V_m found in the literature. We conclude that chloride import through the Na⁺-K⁺-2Cl^- cotransporter and export through Cl^- channels influenced the V_m and the bistable behaviour of mammalian skeletal muscle cells. The possible implication of this bistable behaviour in hypokalaemic periodic paralysis is discussed.

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