HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 543/1/135    most recent 2002.019281v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lewis, M. W. Articles by Travagli, R. A. Search for Related Content PubMed PubMed Citation Articles by Lewis, M. W. Articles by Travagli, R. A.

In vitro and in vivo analysis of the effects of corticotropin releasing factor on rat dorsal vagal complex

Mark W. Lewis *, Gerlinda E. Hermann †, Richard C. Rogers † and R. Alberto Travagli *‡

In vivo and in vitro electrophysiological experiments were performed on the rat dorsal vagal complex (DVC, i.e. nucleus of the tractus solitarius, NTS, and dorsal motor nucleus of the vagus, DMV) to examine the effects of corticotropin releasing hormone (CRF) on the central components of the vago-vagal reflex control of gastric function. When applied to gastrointestinal projecting DMV neurones, CRF (10-300 nM) induced a concentration-dependent membrane depolarization, an increase in action potential firing rate and decrease in amplitude of the action potential afterhyperpolarization (P < 0.05). Pretreatment with the non-selective CRF antagonist, astressin (0.5-1 µM) or the selective CRF₂ receptor antagonist, astressin 2B (500 nM) attenuated the CRF-induced increase in firing rate but did not alter basal discharge rate. CRF (30-300 nM) increased the amplitude of excitatory postsynaptic currents (EPSCs) evoked by stimulation of the NTS (P < 0.05). An alteration in the paired pulse ratio indicated the EPSC's increase occurred due to actions at presynaptic sites. In the in vivo anaesthetized rat preparation, bilateral microinjections (20 fmol in 20 nl for each site) of CRF in the DVC decreased gastric motility in rats pretreated with the muscarinic agonist, bethanecol (P < 0.05). The effects of CRF were abolished by systemic administration of the NOS inhibitor, L-NAME, or by bilateral vagotomy. We concluded that CRF had both a direct and an indirect excitatory effect on DMV neurones via activation of CRF₂ receptors and the decrease in gastric motility observed following microinjection of CRF in the DVC is due to the activation of an inhibitory non-adrenergic non-cholinergic input to the gastrointestinal tract.

This article has been cited by other articles:

				M. A. Herman, M. Niedringhaus, A. Alayan, J. G. Verbalis, N. Sahibzada, and R. A. Gillis Characterization of noradrenergic transmission at the dorsal motor nucleus of the vagus involved in reflex control of fundus tone Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R720 - R729. [Abstract] [Full Text] [PDF]

				C Wallon, P-C Yang, A V Keita, A-C Ericson, D M McKay, P M Sherman, M H Perdue, and J D Soderholm Corticotropin-releasing hormone (CRH) regulates macromolecular permeability via mast cells in normal human colonic biopsies in vitro Gut, January 1, 2008; 57(1): 50 - 58. [Abstract] [Full Text] [PDF]

				M. Niedringhaus, P. G. Jackson, S. R. T. Evans, J. G. Verbalis, R. A. Gillis, and N. Sahibzada Dorsal motor nucleus of the vagus: a site for evoking simultaneous changes in crural diaphragm activity, lower esophageal sphincter pressure, and fundus tone Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R121 - R131. [Abstract] [Full Text] [PDF]

				S. D. Primeaux, M. Tong, and G. M. Holmes Effects of chronic spinal cord injury on body weight and body composition in rats fed a standard chow diet Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1102 - R1109. [Abstract] [Full Text] [PDF]

				D. W. Adelson, H. P. Kosoyan, Y. Wang, J. Z. Steinberg, and Y. Tache Gastric Vagal Efferent Inhibition Evoked by Intravenous CRF Is Unrelated to Simultaneously Recorded Vagal Afferent Activity in Urethane-Anesthetized Rats J Neurophysiol, April 1, 2007; 97(4): 3004 - 3014. [Abstract] [Full Text] [PDF]

				M. T. Cruz, E. C. Murphy, N. Sahibzada, J. G. Verbalis, and R. A. Gillis A reevaluation of the effects of stimulation of the dorsal motor nucleus of the vagus on gastric motility in the rat Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R291 - R307. [Abstract] [Full Text] [PDF]

				R. C. Rogers, G. E. Hermann, and R. A. Travagli Stress and the colon: central-vagal or direct peripheral effect of CRF? Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1535 - R1536. [Full Text] [PDF]

				K. Tsukamoto, Y. Nakade, C. Mantyh, K. Ludwig, T. N. Pappas, and T. Takahashi Peripherally administered CRF stimulates colonic motility via central CRF receptors and vagal pathways in conscious rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1537 - R1541. [Abstract] [Full Text] [PDF]

				G. E. Hermann, R. A. Travagli, and R. C. Rogers Esophageal-gastric relaxation reflex in rat: dual control of peripheral nitrergic and cholinergic transmission Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1570 - R1576. [Abstract] [Full Text] [PDF]

				J. Czimmer, M. Million, and Y. Tache Urocortin 2 acts centrally to delay gastric emptying through sympathetic pathways while CRF and urocortin 1 inhibitory actions are vagal dependent in rats Am J Physiol Gastrointest Liver Physiol, March 1, 2006; 290(3): G511 - G518. [Abstract] [Full Text] [PDF]

				Y. Nakade, D. Tsuchida, H. Fukuda, M. Iwa, T. N. Pappas, and T. Takahashi Restraint stress augments postprandial gastric contractions but impairs antropyloric coordination in conscious rats Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R616 - R624. [Abstract] [Full Text] [PDF]

				K. N. Browning, F. H. Coleman, and R. A. Travagli Effects of pancreatic polypeptide on pancreas-projecting rat dorsal motor nucleus of the vagus neurons Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G209 - G219. [Abstract] [Full Text] [PDF]

				D.-L. Qiu, C.-P. Chu, T. Shirasaka, H. Tsukino, H. Nakao, K. Kato, T. Kunitake, T. Katoh, and H. Kannan Corticotrophin-Releasing Factor Augments the IH in Rat Hypothalamic Paraventricular Nucleus Parvocellular Neurons In Vitro J Neurophysiol, July 1, 2005; 94(1): 226 - 234. [Abstract] [Full Text] [PDF]

				M. Shi, A. R. Jones, M. Ferreira Jr, N. Sahibzada, R. A. Gillis, and J. G. Verbalis Glucose does not activate nonadrenergic, noncholinergic inhibitory neurons in the rat stomach Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2005; 288(3): R742 - R750. [Abstract] [Full Text] [PDF]

				G. E. Hermann, J. S. Nasse, and R. C. Rogers {alpha}-1 adrenergic input to solitary nucleus neurones: calcium oscillations, excitation and gastric reflex control J. Physiol., January 15, 2005; 562(2): 553 - 568. [Abstract] [Full Text] [PDF]

				I. M.-P. y Valenzuela, R. C. Rogers, G. E. Hermann, and R. A. Travagli Norepinephrine effects on identified neurons of the rat dorsal motor nucleus of the vagus Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G333 - G339. [Abstract] [Full Text] [PDF]