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Isotonic force modulates force redevelopment rate of intact frog muscle fibres: evidence for cross-bridge induced thin filament activation

Rene Vandenboom, James D. Hannon and Gary C. Sieck

We tested the hypothesis that force-velocity history modulates thin filament activation, as assessed by the rate of force redevelopment after shortening (+dF/dt_R). The influence of isotonic force on +dF/dt_R was assessed by imposing uniform amplitude (2.55 to 2.15 µm sarcomere^-1) but different speed releases to intact frog muscle fibres during fused tetani. Each release consisted of a contiguous ramp- and step-change in length. Ramp speed was changed from release to release to vary fibre shortening speed from 1.00 (2.76 ± 0.11 µm half-sarcomere^-1 s^-1) to 0.30 of maximum unloaded shortening velocity (V_u), thereby modulating isotonic force from 0 to 0.34 F_o, respectively. The step zeroed force and allowed the fibre to shorten unloaded for a brief period of time prior to force redevelopment. Although peak force redevelopment after different releases was similar, +dF/dt_R increased by 81 ± 6 % (P < 0.05) as fibre shortening speed was reduced from 1.00 V_u. The +dF/dt_R after different releases was strongly correlated with the preceding isotonic force (r = 0.99, P < 0.001). Results from additional experiments showed that the slope of slack test plots produced by systematically increasing the step size that followed each ramp were similar. Thus, isotonic force did not influence V_u (mean: 2.84 ± 0.10 µm half-sarcomere^-1 s^-1, P < 0.05). We conclude that isotonic force modulates +dF/dt_R independent of change in V_u, an outcome consistent with a cooperative influence of attached cross-bridges on thin filament activation that increases cross-bridge attachment rate without alteration to cross-bridge detachment rate.

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