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Effects of perfusion rate on permeability of frog and rat mesenteric microvessels to sodium fluorescein

D. Montermini *†, C. P. Winlove † and C. C. Michel *

The permeability, P_S, to sodium fluorescein (Stokes-Einstein radius = 0.45 nm) has been measured in single mesenteric capillaries of pithed frogs and anaesthetised rats as perfusion velocity, U, was varied over a range from 400 up to 2000-10 000 µm s^-1. P_S increased linearly with U. In 20 frog capillaries, mean (± S.E.M.) P_S (in µm s^-1) = 9.35 (± 1.55)U 10^-5 + 0.244 (± 0.0291). Similarly, in nine rat venules, mean P_S = 1.62 (± 0.385)U 10^-4 + 0.375 (± 0.025). The flow-dependent component of permeability could be reversibly abolished in frog capillaries by superfusing with 100 µM noradrenaline and by superfusing rat venules with the nitric oxide synthase inhibitor, N^G-nitro-L-arginine (20 µM). It was shown that changes in microvascular pressure accompanying changes in U during free perfusion could account for only 15 % of the changes in P_S, i.e. 85 % of the changes in P_S were changes in the permeability coefficient itself. A comparison between the changes in P_S with U and the previously described changes in microvascular permeability to K⁺ with U, suggest that if the flow-dependent component of permeability is modelled as a population of pores of constant size, these have radii of 0.8 nm. Such a pathway would limit flow-dependent permeability to small hydrophilic molecules and have minimal effect on net fluid exchange.

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