HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material All Versions of this Article: 549/3/845    most recent 2002.035923v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Trout, L. Articles by Ballard, S. T. Search for Related Content PubMed PubMed Citation Articles by Trout, L. Articles by Ballard, S. T.

Disruptive effects of anion secretion inhibitors on airway mucus morphology in isolated perfused pig lung

Laura Trout, Mary I. Townsley, Amy L. Bowden, and Stephen T. Ballard

Since anion secretion inhibitors reproduce important aspects of cystic fibrosis (CF) lung disease, the effects of these antagonists on airway mucus morphology were assessed in isolated perfused pig lungs. Maximal inhibitory concentrations of bumetanide and dimethylamiloride, which respectively block Cl^- and HCO₃^- secretion in porcine airways, induced the formation of dense 'plastered' mucus on the airway surface, depletion of periciliary fluid and collapse of cilia. This effect was more pronounced when lungs were also exposed to bethanechol to stimulate submucosal gland secretion, when plastered mucus covered > 98 % of the airway surface. Bethanechol also reduced gland duct mucin content in the absence, but not presence, of the anion secretion inhibitors. Anion secretion inhibitors did not induce measurable increases in goblet cell degranulation. We conclude that inhibition of anion and liquid secretion in porcine lungs disrupts the normal morphology of airway surface mucus, providing further evidence that impaired anion secretion alone could account for critical aspects of CF lung disease.

This article has been cited by other articles:

				C. S. Rogers, W. M. Abraham, K. A. Brogden, J. F. Engelhardt, J. T. Fisher, P. B. McCray Jr., G. McLennan, D. K. Meyerholz, E. Namati, L. S. Ostedgaard, et al. The porcine lung as a potential model for cystic fibrosis Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L240 - L263. [Abstract] [Full Text] [PDF]

				M.-Y. Jian, J. A. King, A.-B. Al-Mehdi, W. Liedtke, and M. I. Townsley High Vascular Pressure-Induced Lung Injury Requires P450 Epoxygenase-Dependent Activation of TRPV4 Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 386 - 392. [Abstract] [Full Text] [PDF]

				R. Tarran, L. Trout, S. H. Donaldson, and R. C. Boucher Soluble Mediators, Not Cilia, Determine Airway Surface Liquid Volume in Normal and Cystic Fibrosis Superficial Airway Epithelia J. Gen. Physiol., April 24, 2006; 127(5): 591 - 604. [Abstract] [Full Text] [PDF]

				S. T. Ballard, J. C. Parker, and C. R. Hamm Restoration of Mucociliary Transport in the Fluid-DepletedTrachea by Surface-Active Instillates Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 500 - 504. [Abstract] [Full Text] [PDF]

				S. T. Ballard and S. K. Inglis Liquid secretion properties of airway submucosal glands J. Physiol., April 1, 2004; 556(1): 1 - 10. [Abstract] [Full Text] [PDF]

				J. J. Wine and N. S. Joo Submucosal Glands and Airway Defense Proceedings of the ATS, January 1, 2004; 1(1): 47 - 53. [Abstract] [Full Text] [PDF]