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Sarcoplasmic reticulum calcium release compared in slow-twitch and fast-twitch fibres of mouse muscle

S. M. Baylor and S. Hollingworth

Experiments were carried out to compare the amplitude and time course of Ca²⁺ release from the sarcoplasmic reticulum (SR) in intact slow-twitch and fast-twitch mouse fibres. Individual fibres within small bundles were injected with furaptra, a low-affinity, rapidly responding Ca²⁺ indicator. In response to a single action potential at 16 °C, the peak amplitude and half-duration of the change in myoplasmic free [Ca²⁺] ([Ca²⁺]) differed significantly between fibre types (slow-twitch: peak amplitude, 9.4 ± 1.0 µM (mean ± S.E.M.); half-duration, 7.7 ± 0.6 ms; fast-twitch: peak amplitude 18.5 ± 0.5 µM; half-duration, 4.9 ± 0.3 ms). SR Ca²⁺ release was estimated from [Ca²⁺] with a computational model that calculated Ca²⁺ binding to the major myoplasmic Ca²⁺ buffers (troponin, ATP and parvalbumin); buffer concentrations and reaction rate constants were adjusted to reflect fibre-type differences. In response to an action potential, the total concentration of released Ca²⁺ ([Ca_T]) and the peak rate of Ca²⁺ release ((d/dt)[Ca_T]) differed about 3-fold between the fibre types (slow-twitch: [Ca_T], 127 ± 7 µM; (d/dt)[Ca_T], 70 ± 6 µM ms^-1; fast-twitch: [Ca_T], 346 ± 6 µM; (d/dt)[Ca_T], 212 ± 4 µM ms^-1). In contrast, the half-duration of (d/dt)[Ca_T] was very similar in the two fibre types (slow-twitch, 1.8 ± 0.1 ms; fast-twitch, 1.6 ± 0.0 ms). When fibres were stimulated with a 5-shock train at 67 Hz, the peaks of (d/dt)[Ca_T] in response to the second and subsequent shocks were much smaller than that due to the first shock; the later peaks, expressed as a fraction of the amplitude of the first peak, were similar in the two fibre types (slow-twitch, 0.2-0.3; fast-twitch, 0.1-0.3). The results support the conclusion that individual SR Ca²⁺ release units function similarly in slow-twitch and fast-twitch mammalian fibres.

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