| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Nerve growth factor (NGF) causes a rapid sensitisation of nociceptive sensory neurones to painful thermal stimuli owing to an action on the heat and capsaicin receptor TRPV1 (formerly known as VR1). We have developed a new technique to study this rapid sensitisation of TRPV1 by monitoring the effects of NGF on the increase in intracellular calcium concentration ([Ca2+]i) following exposure to capsaicin. Brief applications of capsaicin caused a rise in [Ca2+]i, and NGF was found to enhance this rise in 37 % of capsaicin-responsive neurones within 2 min. Pathways responsible for transducing the sensitisation of TRPV1 by TrkA, the NGF receptor, were characterised by observing the effects of inhibitors of key members of NGF-activated second messenger signalling cascades. Specific inhibitors of the ras/MEK (mitogen-activated protein and extracellular signal-regulated kinases) pathway and of phospholipase C did not abolish the NGF-induced sensitisation, but wortmannin, a specific inhibitor of phosphatidylinositol-3-kinase (PI3K), totally abolished the effect of NGF. Pharmacological blockade of protein kinase C (PKC) or calcium-calmodulin-dependent protein kinase II (CaMK II) activation also prevented NGF-induced sensitisation, while blockade of protein kinase A (PKA) was without effect. These data indicate that the crucial early pathway activated by NGF involves PI3K, while PKC and CaMK II are also involved, probably at subsequent stages of the NGF-activated signalling pathway.
This article has been cited by other articles:
|
|
 |
|
  C. S. Surowy, T. R. Neelands, B. R. Bianchi, S. McGaraughty, R. El Kouhen, P. Han, K. L. Chu, H. A. McDonald, M. Vos, W. Niforatos, et al. (R)-(5-tert-Butyl-2,3-dihydro-1H-inden-1-yl)-3-(1H-indazol-4-yl)-urea (ABT-102) Blocks Polymodal Activation of Transient Receptor Potential Vanilloid 1 Receptors in Vitro and Heat-Evoked Firing of Spinal Dorsal Horn Neurons in Vivo J. Pharmacol. Exp. Ther., September 1, 2008; 326(3): 879 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhu, P. Xu, F. X. Cuascut, A. K. Hall, and G. S. Oxford Activin Acutely Sensitizes Dorsal Root Ganglion Neurons and Induces Hyperalgesia via PKC-Mediated Potentiation of Transient Receptor Potential Vanilloid I J. Neurosci., December 12, 2007; 27(50): 13770 - 13780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, C.-L. Cang, Y. Kawasaki, L.-L. Liang, Y.-Q. Zhang, R.-R. Ji, and Z.-Q. Zhao Neurokinin-1 Receptor Enhances TRPV1 Activity in Primary Sensory Neurons via PKC{varepsilon}: A Novel Pathway for Heat Hyperalgesia J. Neurosci., October 31, 2007; 27(44): 12067 - 12077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Thacker, A. K. Clark, F. Marchand, and S. B. McMahon Pathophysiology of Peripheral Neuropathic Pain: Immune Cells and Molecules Anesth. Analg., September 1, 2007; 105(3): 838 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Akopian, N. B. Ruparel, N. A. Jeske, and K. M. Hargreaves Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization J. Physiol., August 15, 2007; 583(1): 175 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D'Arco, R. Giniatullin, M. Simonetti, A. Fabbro, A. Nair, A. Nistri, and E. Fabbretti Neutralization of Nerve Growth Factor Induces Plasticity of ATP-Sensitive P2X3 Receptors of Nociceptive Trigeminal Ganglion Neurons J. Neurosci., August 1, 2007; 27(31): 8190 - 8201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sikand and L. S. Premkumar Potentiation of glutamatergic synaptic transmission by protein kinase C-mediated sensitization of TRPV1 at the first sensory synapse J. Physiol., June 1, 2007; 581(2): 631 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ugolini, S. Marinelli, S. Covaceuszach, A. Cattaneo, and F. Pavone The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain PNAS, February 20, 2007; 104(8): 2985 - 2990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Nicol and M. R. Vasko Unraveling the Story of NGF-mediated Sensitization of Nociceptive Sensory Neurons: ON or OFF the Trks? Mol. Interv., February 1, 2007; 7(1): 26 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Stein, C. A. Ufret-Vincenty, L. Hua, L. F. Santana, and S. E. Gordon Phosphoinositide 3-Kinase Binds to TRPV1 and Mediates NGF-stimulated TRPV1 Trafficking to the Plasma Membrane J. Gen. Physiol., November 1, 2006; 128(5): 509 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang and P. A. McNaughton Why Pain Gets Worse: The Mechanism of Heat Hyperalgesia J. Gen. Physiol., November 1, 2006; 128(5): 491 - 493. [Full Text] [PDF]
|
|
|
|
|
|
T. Ohta, Y. Ikemi, M. Murakami, T. Imagawa, K.-i. Otsuguro, and S. Ito Potentiation of transient receptor potential V1 functions by the activation of metabotropic 5-HT receptors in rat primary sensory neurons J. Physiol., November 1, 2006; 576(3): 809 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Malin, D. C. Molliver, H. R. Koerber, P. Cornuet, R. Frye, K. M. Albers, and B. M. Davis Glial Cell Line-Derived Neurotrophic Factor Family Members Sensitize Nociceptors In Vitro and Produce Thermal Hyperalgesia In Vivo. J. Neurosci., August 15, 2006; 26(33): 8588 - 8599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. H. Zhang, M. R. Vasko, and G. D. Nicol Intracellular sphingosine 1-phosphate mediates the increased excitability produced by nerve growth factor in rat sensory neurons J. Physiol., August 15, 2006; 575(1): 101 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Diogenes, A. M. Patwardhan, N. A. Jeske, N. B. Ruparel, V. Goffin, A. N. Akopian, and K. M. Hargreaves Prolactin Modulates TRPV1 in Female Rat Trigeminal Sensory Neurons J. Neurosci., August 2, 2006; 26(31): 8126 - 8136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Watson, M. S. Fahey, E. van den Worm, F. Engels, F. P. Nijkamp, P. Stroemer, S. McMahon, S. J. Allen, and D. Dawbarn TrkAd5: A Novel Therapeutic Agent for Treatment of Inflammatory Pain and Asthma J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1122 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Liu, C. Zhang, and F. Qin Functional Recovery from Desensitization of Vanilloid Receptor TRPV1 Requires Resynthesis of Phosphatidylinositol 4,5-Bisphosphate J. Neurosci., May 11, 2005; 25(19): 4835 - 4843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hong and J. W. Wiley Early Painful Diabetic Neuropathy Is Associated with Differential Changes in the Expression and Function of Vanilloid Receptor 1 J. Biol. Chem., January 7, 2005; 280(1): 618 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhu, S. M. Galoyan, J. C. Petruska, G. S. Oxford, and L. M. Mendell A Developmental Switch in Acute Sensitization of Small Dorsal Root Ganglion (DRG) Neurons to Capsaicin or Noxious Heating by NGF J Neurophysiol, November 1, 2004; 92(5): 3148 - 3152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Liu, W. Ma, S. Ryu, and F. Qin Inhibitory modulation of distal C-terminal on protein kinase C-dependent phospho-regulation of rat TRPV1 receptors J. Physiol., November 1, 2004; 560(3): 627 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-R. Ji and G. Strichartz Cell Signaling and the Genesis of Neuropathic Pain Sci. Signal., September 28, 2004; 2004(252): re14 - re14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Y. Zhuang, H. Xu, D. E. Clapham, and R.-R. Ji Phosphatidylinositol 3-Kinase Activates ERK in Primary Sensory Neurons and Mediates Inflammatory Heat Hyperalgesia through TRPV1 Sensitization J. Neurosci., September 22, 2004; 24(38): 8300 - 8309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Morenilla-Palao, R. Planells-Cases, N. Garcia-Sanz, and A. Ferrer-Montiel Regulated Exocytosis Contributes to Protein Kinase C Potentiation of Vanilloid Receptor Activity J. Biol. Chem., June 11, 2004; 279(24): 25665 - 25672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Deval, M. Salinas, A. Baron, E. Lingueglia, and M. Lazdunski ASIC2b-dependent Regulation of ASIC3, an Essential Acid-sensing Ion Channel Subunit in Sensory Neurons via the Partner Protein PICK-1 J. Biol. Chem., May 7, 2004; 279(19): 19531 - 19539. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
