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This Article Full Text Full Text (PDF) All Versions of this Article: 552/1/325    most recent jphysiol.2003.050310v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nita, D. A. Articles by Amzica, F. Search for Related Content PubMed PubMed Citation Articles by Nita, D. A. Articles by Amzica, F.

Hyperpolarisation rectification in cat lateral geniculate neurons modulated by intact corticothalamic projections

D. A. Nita, M. Steriade and F. Amzica

The intrinsic properties of thalamic neurons are influenced by synaptic activities in ascending pathways and corticofugal projections, as well as by the actions of neurotransmitters released by generalised modulatory systems. We focused on the effects of corticothalamic projections on the hyperpolarisation-activated cation current I_h. Intracellular recordings of thalamocortical neurons in the dorsal lateral geniculate (dLG) nucleus were performed in cats under ketamine-xylazine anaesthesia. At variance with the conventional way of recording intracellularly from thalamic neurons after partial or total ablation of the grey and white matter overlying the dLG, we preserved intact corticothalamic neuronal loops. Stimulating electrodes inserted into the optic tract and light-emitting-diodes as photic stimulation were used to identify the dLG neurons. The expression of the depolarising sag due to I_h depended on the state of cortical networks. Thalamic dLG I_h, induced by hyperpolarising current steps, was detected during the periods of cortical disfacilitation that occur during the cortical slow (< 1 Hz) oscillation, whereas I_h was absent during the active (depolarised) periods. The possibility that the excitatory corticothalamic projections could preclude the generation of the I_h was tested by applying a concentrated K⁺ solution (3 M) to the primary visual cortex. The same dLG neurons that did not display I_h before application of K⁺ were able to produce hyperpolarisation-activated depolarising sags during K⁺-induced cortical depression. Our data suggest that the thalamic clock-like delta oscillation, which results from an interplay between I_h and the low-threshold calcium current (I_T), as described in preparations without cerebral cortex, is prevented in dLG neurons when corticothalamic loops are intact.