HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 552/2/449    most recent jphysiol.2003.047373v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yeung, E. W. Articles by Allen, D. G. Search for Related Content PubMed PubMed Citation Articles by Yeung, E. W. Articles by Allen, D. G.

Gadolinium reduces short-term stretch-induced muscle damage in isolated mdx mouse muscle fibres

Ella W. Yeung *, Stewart I. Head † and David G. Allen ‡

Duchenne muscular dystrophy is a lethal muscle disease caused by absence of the protein dystrophin which is part of a glycoprotein complex located on the intracellular surface of the surface membrane. The precise function of dystrophin and the reason why its absence causes severe muscle damage are unclear. Stretch-induced muscle damage is well recognised in normal muscle and is more severe in muscles from animals lacking dystrophin (mdx mice). It has been proposed that stretch-induced damage underlies the progression of damage in muscular dystrophy. In the present study we confirm that single fibres from mdx muscle are more susceptible to stretch-induced damage and show that there is an associated rise in intracellular sodium concentration ([Na⁺]_i) which is greater than in wild-type mice. We show that this rise in [Na⁺]_i can be prevented by Gd³⁺, which is an established blocker of stretch-activated channels. mdx fibres have a higher than normal resting [Na⁺]_i and this is also reduced by Gd³⁺. If Gd³⁺ is applied over the period in which [Na⁺]_i rises following stretched contraction, it prevents one component of the reduced force. The other component of reduced force is caused by inhomogeneity of sarcomeres and can be minimised by stretching the muscle to its new optimum length. These experiments show that part of the short-term damage caused by stretch in mdx fibres can be prevented by blocking stretch-activated channels.

This article has been cited by other articles:

				C. Hirn, G. Shapovalov, O. Petermann, E. Roulet, and U. T. Ruegg Nav1.4 Deregulation in Dystrophic Skeletal Muscle Leads to Na+ Overload and Enhanced Cell Death J. Gen. Physiol., July 28, 2008; 132(2): 199 - 208. [Abstract] [Full Text] [PDF]

				T. Sonobe, T. Inagaki, D. C. Poole, and Y. Kano Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1329 - R1337. [Abstract] [Full Text] [PDF]

				G. D'Antona, L. Brocca, O. Pansarasa, C. Rinaldi, R. Tupler, and R. Bottinelli Structural and functional alterations of muscle fibres in the novel mouse model of facioscapulohumeral muscular dystrophy J. Physiol., November 1, 2007; 584(3): 997 - 1009. [Abstract] [Full Text] [PDF]

				Y. Iwata, Y. Katanosaka, T. Hisamitsu, and S. Wakabayashi Enhanced Na+/H+ Exchange Activity Contributes to the Pathogenesis of Muscular Dystrophy via Involvement of P2 Receptors Am. J. Pathol., November 1, 2007; 171(5): 1576 - 1587. [Abstract] [Full Text] [PDF]

				S. Chan, S. I. Head, and J. W. Morley Branched fibers in dystrophic mdx muscle are associated with a loss of force following lengthening contractions Am J Physiol Cell Physiol, September 1, 2007; 293(3): C985 - C992. [Abstract] [Full Text] [PDF]

				T. M. Suchyna and F. Sachs Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubes J. Physiol., May 15, 2007; 581(1): 369 - 387. [Abstract] [Full Text] [PDF]

				E. E. Spangenburg and T. A. McBride Inhibition of stretch-activated channels during eccentric muscle contraction attenuates p70S6K activation J Appl Physiol, January 1, 2006; 100(1): 129 - 135. [Abstract] [Full Text] [PDF]

				D. G Allen, N. P Whitehead, and E. W Yeung Mechanisms of stretch-induced muscle damage in normal and dystrophic muscle: role of ionic changes J. Physiol., September 15, 2005; 567(3): 723 - 735. [Abstract] [Full Text] [PDF]

				O Prasartwuth, J. L Taylor, and S. C Gandevia Maximal force, voluntary activation and muscle soreness after eccentric damage to human elbow flexor muscles J. Physiol., August 15, 2005; 567(1): 337 - 348. [Abstract] [Full Text] [PDF]

				E. W. Yeung, N. P. Whitehead, T. M. Suchyna, P. A. Gottlieb, F. Sachs, and D. G. Allen Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouse J. Physiol., January 15, 2005; 562(2): 367 - 380. [Abstract] [Full Text] [PDF]

				S. I. Head, A. J. Bakker, and G. Liangas EDL and soleus muscles of the C57BL6J/dy2j laminin-{alpha}2-deficient dystrophic mouse are not vulnerable to eccentric contractions Exp Physiol, September 1, 2004; 89(5): 531 - 539. [Abstract] [Full Text] [PDF]