HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 553/1/243    most recent jphysiol.2003.044222v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brecht, M. Articles by Sakmann, B. Search for Related Content PubMed PubMed Citation Articles by Brecht, M. Articles by Sakmann, B.

Dynamic receptive fields of reconstructed pyramidal cells in layers 3 and 2 of rat somatosensory barrel cortex

Michael Brecht, Arnd Roth and Bert Sakmann

Whole-cell voltage recordings were made in vivo from subsequently reconstructed pyramidal neurons (n = 30) in layer 3 (L3) and layer 2 (L2) of the barrel cortex of urethane-anaesthetised rats. Average resting membrane potentials were well below (15-40 mV) action potential (AP) initiation threshold. The average spontaneous AP activity (0.068 ± 0.22 APs s^-1) was low. Principal whisker (PW) deflections evoked postsynaptic potentials (PSPs) in almost all cells of a PW column but evoked AP activity (0.031 ± 0.056 APs per PW stimulus 6 deg deflection) was low indicating 'sparse' coding by APs. Barrel-related cells (n = 16) have their soma located above a barrel and project their main axon through the barrel whereas septum-related cells (n = 8) are located above and project their main axon through the septum between barrels. Both classes of cell had broad subthreshold receptive fields (RFs) which comprised a PW and several (> 8) surround whiskers (SuW). Barrel-related cells had shorter PSP onset latencies (9.6 ± 4.6 ms) and larger amplitude PW stimulus responses (9.1 ± 4.5 mV) than septum-related cells (23.3 ± 16.5 ms and 5.0 ± 2.8 mV, respectively). The dendritic fields of barrel-related cells were restricted, in the horizontal plane, to the PW column width. Their axonal arbors projected horizontally into several SuW columns, preferentially those representing whiskers of the same row, suggesting that they are the major anatomical substrate for the broad subthreshold RFs. In barrel-related cells the response time course varied with whisker position and subthreshold RFs were highly dynamic, expanding in size from narrow single-whisker to broad multi-whisker RFs, elongated along rows within 10-150 ms following a deflection. The response time course in septum-related cells was much longer and almost independent of whisker position. Their broad subthreshold RF suggests that L2/3 cells integrate PSPs from several barrel columns. We conclude that the lemniscal (barrel-related) and paralemniscal (septum-related) afferent inputs remain anatomically and functionally segregated in L2/3.

This article has been cited by other articles:

				R. D. Frostig, Y. Xiong, C. H. Chen-Bee, E. Kvasnak, and J. Stehberg Large-Scale Organization of Rat Sensorimotor Cortex Based on a Motif of Large Activation Spreads J. Neurosci., December 3, 2008; 28(49): 13274 - 13284. [Abstract] [Full Text] [PDF]

				J. E. Heiss, Y. Katz, E. Ganmor, and I. Lampl Shift in the Balance between Excitation and Inhibition during Sensory Adaptation of S1 Neurons J. Neurosci., December 3, 2008; 28(49): 13320 - 13330. [Abstract] [Full Text] [PDF]

				M. Helmstaedter, B. Sakmann, and D. Feldmeyer Neuronal Correlates of Local, Lateral, and Translaminar Inhibition with Reference to Cortical Columns Cereb Cortex, October 1, 2008; (2008) bhn141v1. [Abstract] [Full Text] [PDF]

				M. Helmstaedter, J. F. Staiger, B. Sakmann, and D. Feldmeyer Efficient Recruitment of Layer 2/3 Interneurons by Layer 4 Input in Single Columns of Rat Somatosensory Cortex J. Neurosci., August 13, 2008; 28(33): 8273 - 8284. [Abstract] [Full Text] [PDF]

				C. M. Niell and M. P. Stryker Highly Selective Receptive Fields in Mouse Visual Cortex J. Neurosci., July 23, 2008; 28(30): 7520 - 7536. [Abstract] [Full Text] [PDF]

				P. Broser, V. Grinevich, P. Osten, B. Sakmann, and D. J. Wallace Critical Period Plasticity of Axonal Arbors of Layer 2/3 Pyramidal Neurons in Rat Somatosensory Cortex: Layer-Specific Reduction of Projections into Deprived Cortical Columns Cereb Cortex, July 1, 2008; 18(7): 1588 - 1603. [Abstract] [Full Text] [PDF]

				I. R. Winship and T. H. Murphy In Vivo Calcium Imaging Reveals Functional Rewiring of Single Somatosensory Neurons after Stroke J. Neurosci., June 25, 2008; 28(26): 6592 - 6606. [Abstract] [Full Text] [PDF]

				B. de Celis Alonso, A. S. Lowe, J. P. Dear, K. C. Lee, S. C. R. Williams, and G. T. Finnerty Sensory Inputs from Whisking Movements Modify Cortical Whisker Maps Visualized with Functional Magnetic Resonance Imaging Cereb Cortex, June 1, 2008; 18(6): 1314 - 1325. [Abstract] [Full Text] [PDF]

				P. J. Drew and D. E. Feldman Intrinsic Signal Imaging of Deprivation-Induced Contraction of Whisker Representations in Rat Somatosensory Cortex Cereb Cortex, May 30, 2008; (2008) bhn085v1. [Abstract] [Full Text] [PDF]

				B. Kuhn, W. Denk, and R. M. Bruno In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness PNAS, May 27, 2008; 105(21): 7588 - 7593. [Abstract] [Full Text] [PDF]

				V. Pawlak and J. N. D. Kerr Dopamine Receptor Activation Is Required for Corticostriatal Spike-Timing-Dependent Plasticity J. Neurosci., March 5, 2008; 28(10): 2435 - 2446. [Abstract] [Full Text] [PDF]

				A. Suppa, M. Bologna, F. Gilio, C. Lorenzano, J. C. Rothwell, and A. Berardelli Preconditioning Repetitive Transcranial Magnetic Stimulation of Premotor Cortex Can Reduce But Not Enhance Short-Term Facilitation of Primary Motor Cortex J Neurophysiol, February 1, 2008; 99(2): 564 - 570. [Abstract] [Full Text] [PDF]

				A. Frick, D. Feldmeyer, M. Helmstaedter, and B. Sakmann Monosynaptic Connections between Pairs of L5A Pyramidal Neurons in Columns of Juvenile Rat Somatosensory Cortex Cereb Cortex, February 1, 2008; 18(2): 397 - 406. [Abstract] [Full Text] [PDF]

				J. N. D. Kerr, C. P. J. de Kock, D. S. Greenberg, R. M. Bruno, B. Sakmann, and F. Helmchen Spatial Organization of Neuronal Population Responses in Layer 2/3 of Rat Barrel Cortex J. Neurosci., November 28, 2007; 27(48): 13316 - 13328. [Abstract] [Full Text] [PDF]

				L. Sarid, R. Bruno, B. Sakmann, I. Segev, and D. Feldmeyer Modeling a layer 4-to-layer 2/3 module of a single column in rat neocortex: Interweaving in vitro and in vivo experimental observations PNAS, October 9, 2007; 104(41): 16353 - 16358. [Abstract] [Full Text] [PDF]

				P. J. Drew and D. E. Feldman Representation of Moving Wavefronts of Whisker Deflection in Rat Somatosensory Cortex J Neurophysiol, September 1, 2007; 98(3): 1566 - 1580. [Abstract] [Full Text] [PDF]

				P.-O. Polack, I. Guillemain, E. Hu, C. Deransart, A. Depaulis, and S. Charpier Deep Layer Somatosensory Cortical Neurons Initiate Spike-and-Wave Discharges in a Genetic Model of Absence Seizures J. Neurosci., June 13, 2007; 27(24): 6590 - 6599. [Abstract] [Full Text] [PDF]

				C. P. J. de Kock, R. M. Bruno, H. Spors, and B. Sakmann Layer- and cell-type-specific suprathreshold stimulus representation in rat primary somatosensory cortex J. Physiol., May 15, 2007; 581(1): 139 - 154. [Abstract] [Full Text] [PDF]

				T. Berger, A. Borgdorff, S. Crochet, F. B. Neubauer, S. Lefort, B. Fauvet, I. Ferezou, A. Carleton, H.-R. Luscher, and C. C. H. Petersen Combined Voltage and Calcium Epifluorescence Imaging In Vitro and In Vivo Reveals Subthreshold and Suprathreshold Dynamics of Mouse Barrel Cortex J Neurophysiol, May 1, 2007; 97(5): 3751 - 3762. [Abstract] [Full Text] [PDF]

				A. Devor, P. Tian, N. Nishimura, I. C. Teng, E. M. C. Hillman, S. N. Narayanan, I. Ulbert, D. A. Boas, D. Kleinfeld, and A. M. Dale Suppressed Neuronal Activity and Concurrent Arteriolar Vasoconstriction May Explain Negative Blood Oxygenation Level-Dependent Signal J. Neurosci., April 18, 2007; 27(16): 4452 - 4459. [Abstract] [Full Text] [PDF]

				A. J. Borgdorff, J. F. A. Poulet, and C. C. H. Petersen Facilitating Sensory Responses in Developing Mouse Somatosensory Barrel Cortex J Neurophysiol, April 1, 2007; 97(4): 2992 - 3003. [Abstract] [Full Text] [PDF]

				C. E. J. Cheetham, M. S. L. Hammond, C. E. J. Edwards, and G. T. Finnerty Sensory Experience Alters Cortical Connectivity and Synaptic Function Site Specifically J. Neurosci., March 28, 2007; 27(13): 3456 - 3465. [Abstract] [Full Text] [PDF]

				C. Quairiaux, M. Armstrong-James, and E. Welker Modified Sensory Processing in the Barrel Cortex of the Adult Mouse After Chronic Whisker Stimulation J Neurophysiol, March 1, 2007; 97(3): 2130 - 2147. [Abstract] [Full Text] [PDF]

				A. Devor, A. Trevelyan, and D. Kleinfeld Is There a Common Origin to Surround-Inhibition as Seen Through Electrical Activity Versus Hemodynamic Changes? Focus on "Duration-Dependent Response in SI to Vibrotactile Stimulation in Squirrel Monkey" J Neurophysiol, March 1, 2007; 97(3): 1880 - 1882. [Full Text] [PDF]

				V. Jacob, D. J. Brasier, I. Erchova, D. Feldman, and D. E. Shulz Spike Timing-Dependent Synaptic Depression in the In Vivo Barrel Cortex of the Rat J. Neurosci., February 7, 2007; 27(6): 1271 - 1284. [Abstract] [Full Text] [PDF]

				Y. Katz, J. E. Heiss, and I. Lampl Cross-Whisker Adaptation of Neurons in the Rat Barrel Cortex J. Neurosci., December 20, 2006; 26(51): 13363 - 13372. [Abstract] [Full Text] [PDF]

				T. Nevian and B. Sakmann Spine Ca2+ Signaling in Spike-Timing-Dependent Plasticity J. Neurosci., October 25, 2006; 26(43): 11001 - 11013. [Abstract] [Full Text] [PDF]

				D. Feldmeyer, J. Lubke, and B. Sakmann Efficacy and connectivity of intracolumnar pairs of layer 2/3 pyramidal cells in the barrel cortex of juvenile rats J. Physiol., September 1, 2006; 575(2): 583 - 602. [Abstract] [Full Text] [PDF]

				J. Waters and F. Helmchen Background synaptic activity is sparse in neocortex. J. Neurosci., August 9, 2006; 26(32): 8267 - 8277. [Abstract] [Full Text] [PDF]

				E. F. Civillico and D. Contreras Integration of Evoked Responses in Supragranular Cortex Studied With Optical Recordings In Vivo J Neurophysiol, July 1, 2006; 96(1): 336 - 351. [Abstract] [Full Text] [PDF]

				J. Hipp, E. Arabzadeh, E. Zorzin, J. Conradt, C. Kayser, M. E. Diamond, and P. Konig Texture Signals in Whisker Vibrations J Neurophysiol, March 1, 2006; 95(3): 1792 - 1799. [Abstract] [Full Text] [PDF]

				E. Foeller, T. Celikel, and D. E. Feldman Inhibitory Sharpening of Receptive Fields Contributes to Whisker Map Plasticity in Rat Somatosensory Cortex J Neurophysiol, December 1, 2005; 94(6): 4387 - 4400. [Abstract] [Full Text] [PDF]

				G. M. G. Shepherd and K. Svoboda Laminar and Columnar Organization of Ascending Excitatory Projections to Layer 2/3 Pyramidal Neurons in Rat Barrel Cortex J. Neurosci., June 15, 2005; 25(24): 5670 - 5679. [Abstract] [Full Text] [PDF]

				D. Feldmeyer, A. Roth, and B. Sakmann Monosynaptic Connections between Pairs of Spiny Stellate Cells in Layer 4 and Pyramidal Cells in Layer 5A Indicate That Lemniscal and Paralemniscal Afferent Pathways Converge in the Infragranular Somatosensory Cortex J. Neurosci., March 30, 2005; 25(13): 3423 - 3431. [Abstract] [Full Text] [PDF]

				W. B. Wilent and D. Contreras Stimulus-Dependent Changes in Spike Threshold Enhance Feature Selectivity in Rat Barrel Cortex Neurons J. Neurosci., March 16, 2005; 25(11): 2983 - 2991. [Abstract] [Full Text] [PDF]

				A. Devor, I. Ulbert, A. K. Dunn, S. N. Narayanan, S. R. Jones, M. L. Andermann, D. A. Boas, and A. M. Dale Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity PNAS, March 8, 2005; 102(10): 3822 - 3827. [Abstract] [Full Text] [PDF]

				T. Dittgen, A. Nimmerjahn, S. Komai, P. Licznerski, J. Waters, T. W. Margrie, F. Helmchen, W. Denk, M. Brecht, and P. Osten Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo PNAS, December 28, 2004; 101(52): 18206 - 18211. [Abstract] [Full Text] [PDF]

				J. Waters and F. Helmchen Boosting of Action Potential Backpropagation by Neocortical Network Activity In Vivo J. Neurosci., December 8, 2004; 24(49): 11127 - 11136. [Abstract] [Full Text] [PDF]

				Y. Zhu, R. L. Stornetta, and J. J. Zhu Chandelier Cells Control Excessive Cortical Excitation: Characteristics of Whisker-Evoked Synaptic Responses of Layer 2/3 Nonpyramidal and Pyramidal Neurons J. Neurosci., June 2, 2004; 24(22): 5101 - 5108. [Abstract] [Full Text] [PDF]

				C. C. H. Petersen, M. Brecht, T. T. G. Hahn, and B. Sakmann Synaptic Changes in Layer 2/3 Underlying Map Plasticity of Developing Barrel Cortex Science, April 30, 2004; 304(5671): 739 - 742. [Abstract] [Full Text] [PDF]

				W. B. Wilent and D. Contreras Synaptic Responses to Whisker Deflections in Rat Barrel Cortex as a Function of Cortical Layer and Stimulus Intensity J. Neurosci., April 21, 2004; 24(16): 3985 - 3998. [Abstract] [Full Text] [PDF]

				I. D. Manns, B. Sakmann, and M. Brecht Sub- and suprathreshold receptive field properties of pyramidal neurones in layers 5A and 5B of rat somatosensory barrel cortex J. Physiol., April 15, 2004; 556(2): 601 - 622. [Abstract] [Full Text] [PDF]

				Y. Zhu and J. J. Zhu Rapid Arrival and Integration of Ascending Sensory Information in Layer 1 Nonpyramidal Neurons and Tuft Dendrites of Layer 5 Pyramidal Neurons of the Neocortex J. Neurosci., February 11, 2004; 24(6): 1272 - 1279. [Abstract] [Full Text] [PDF]