HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 553/1/303    most recent jphysiol.2003.054171v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rose, A. J. Articles by Hargreaves, M. Search for Related Content PubMed PubMed Citation Articles by Rose, A. J. Articles by Hargreaves, M.

Exercise increases Ca²⁺-calmodulin-dependent protein kinase II activity in human skeletal muscle

Adam J. Rose and Mark Hargreaves

There is evidence in rodents that Ca²⁺-calmodulin-dependent protein kinase II (CaMKII) activity is higher in contracting skeletal muscle, and this kinase may regulate skeletal muscle function and metabolism during exercise. To investigate the effect of exercise on CaMKII in human skeletal muscle, healthy men (n = 8) performed cycle ergometer exercise for 40 min at 76 ± 1 % peak pulmonary O₂ uptake ( _O2peak), with skeletal muscle samples taken at rest and after 5 and 40 min of exercise. CaMKII expression and activities were examined by immunoblotting and in vitro kinase assays, respectively. There were no differences in maximal (+ Ca²⁺, CaM) CaMKII activity during exercise compared with rest. Autonomous (- Ca²⁺, CaM) CaMKII activity was 9 ± 1 % of maximal at rest, remained unchanged at 5 min, and increased to 17 ± 1 % (P < 0.01) at 40 min. CaMKII autophosphorylation at Thr²⁸⁷ was 50-70 % higher during exercise, with no differences in CaMKII expression. The effect of maximal aerobic exercise on CaMKII was also examined (n = 9), with 0.7- to 1.5-fold increases in autonomous CaMKII activity, but no change in maximal CaMKII activity. CaMKIV was not detected in human skeletal muscle. In summary, exercise increases the activity of CaMKII in skeletal muscle, suggesting that it may have a role in regulating skeletal muscle function and metabolism during exercise in humans.

This article has been cited by other articles:

				S. Park, T. L. Scheffler, A. M. Gunawan, H. Shi, C. Zeng, K. M. Hannon, A. L. Grant, and D. E. Gerrard Chronic elevated calcium blocks AMPK-induced GLUT-4 expression in skeletal muscle Am J Physiol Cell Physiol, January 1, 2009; 296(1): C106 - C115. [Abstract] [Full Text] [PDF]

				B. Benziane, T. J. Burton, B. Scanlan, D. Galuska, B. J. Canny, A. V. Chibalin, J. R. Zierath, and N. K. Stepto Divergent cell signaling after short-term intensified endurance training in human skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2008; 295(6): E1427 - E1438. [Abstract] [Full Text] [PDF]

				J. A. H. Smith, T. A. Kohn, A. K. Chetty, and E. O. Ojuka CaMK activation during exercise is required for histone hyperacetylation and MEF2A binding at the MEF2 site on the Glut4 gene Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E698 - E704. [Abstract] [Full Text] [PDF]

				M. A. Raney and L. P. Turcotte Evidence for the involvement of CaMKII and AMPK in Ca2+-dependent signaling pathways regulating FA uptake and oxidation in contracting rodent muscle J Appl Physiol, May 1, 2008; 104(5): 1366 - 1373. [Abstract] [Full Text] [PDF]

				D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]

				D. G. Allen, G. D. Lamb, and H. Westerblad Impaired calcium release during fatigue J Appl Physiol, January 1, 2008; 104(1): 296 - 305. [Abstract] [Full Text] [PDF]

				M. Ekmark, Z. A. Rana, G. Stewart, D. G. Hardie, and K. Gundersen De-phosphorylation of MyoD is linking nerve-evoked activity to fast myosin heavy chain expression in rodent adult skeletal muscle J. Physiol., October 15, 2007; 584(2): 637 - 650. [Abstract] [Full Text] [PDF]

				A. J. Rose, C. Frosig, B. Kiens, J. F. P. Wojtaszewski, and E. A. Richter Effect of endurance exercise training on Ca2+ calmodulin-dependent protein kinase II expression and signalling in skeletal muscle of humans J. Physiol., September 1, 2007; 583(2): 785 - 795. [Abstract] [Full Text] [PDF]

				D. C. Wright, P. C. Geiger, D.-H. Han, T. E. Jones, and J. O. Holloszy Calcium Induces Increases in Peroxisome Proliferator-activated Receptor {gamma} Coactivator-1{alpha} and Mitochondrial Biogenesis by a Pathway Leading to p38 Mitogen-activated Protein Kinase Activation J. Biol. Chem., June 29, 2007; 282(26): 18793 - 18799. [Abstract] [Full Text] [PDF]

				A. J. Rose, T. J. Alsted, J. B. Kobbero, and E. A. Richter Regulation and function of Ca2+-calmodulin-dependent protein kinase II of fast-twitch rat skeletal muscle J. Physiol., May 1, 2007; 580(3): 993 - 1005. [Abstract] [Full Text] [PDF]

				T. E. Jensen, A. J. Rose, S. B. Jorgensen, N. Brandt, P. Schjerling, J. F. P. Wojtaszewski, and E. A. Richter Possible CaMKK-dependent regulation of AMPK phosphorylation and glucose uptake at the onset of mild tetanic skeletal muscle contraction Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1308 - E1317. [Abstract] [Full Text] [PDF]

				J. A. Valdes, J. Hidalgo, J. L. Galaz, N. Puentes, M. Silva, E. Jaimovich, and M. A. Carrasco NF-{kappa}B activation by depolarization of skeletal muscle cells depends on ryanodine and IP3 receptor-mediated calcium signals Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1960 - C1970. [Abstract] [Full Text] [PDF]

				A. Katz Modulation of glucose transport in skeletal muscle by reactive oxygen species J Appl Physiol, April 1, 2007; 102(4): 1671 - 1676. [Abstract] [Full Text] [PDF]

				J. A. H. Smith, M. Collins, L. A. Grobler, C. J. Magee, and E. O. Ojuka Exercise and CaMK activation both increase the binding of MEF2A to the Glut4 promoter in skeletal muscle in vivo Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E413 - E420. [Abstract] [Full Text] [PDF]

				S. B. Jorgensen, J. T. Treebak, B. Viollet, P. Schjerling, S. Vaulont, J. F. P. Wojtaszewski, and E. A. Richter Role of AMPK{alpha}2 in basal, training-, and AICAR-induced GLUT4, hexokinase II, and mitochondrial protein expression in mouse muscle Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E331 - E339. [Abstract] [Full Text] [PDF]

				G. N. Kraniou, D. Cameron-Smith, and M. Hargreaves Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity J Appl Physiol, September 1, 2006; 101(3): 934 - 937. [Abstract] [Full Text] [PDF]

				A. J. Rose, B. Kiens, and E. A. Richter Ca2+-calmodulin-dependent protein kinase expression and signalling in skeletal muscle during exercise J. Physiol., August 1, 2006; 574(3): 889 - 903. [Abstract] [Full Text] [PDF]

				D. C. Wright, P. C. Geiger, D.-H. Han, and J. O. Holloszy Are tyrosine kinases involved in mediating contraction-stimulated muscle glucose transport? Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E123 - E128. [Abstract] [Full Text] [PDF]

				R. C. Ho, M. F. Hirshman, Y. Li, D. Cai, J. R. Farmer, W. G. Aschenbach, C. A. Witczak, S. E. Shoelson, and L. J. Goodyear Regulation of I{kappa}B kinase and NF-{kappa}B in contracting adult rat skeletal muscle Am J Physiol Cell Physiol, October 1, 2005; 289(4): C794 - C801. [Abstract] [Full Text] [PDF]

				E. R. Chin Role of Ca2+/calmodulin-dependent kinases in skeletal muscle plasticity J Appl Physiol, August 1, 2005; 99(2): 414 - 423. [Abstract] [Full Text] [PDF]

				A. J. Rose and E. A. Richter Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated? Physiology, August 1, 2005; 20(4): 260 - 270. [Abstract] [Full Text] [PDF]

				Y. Liu, W. R. Randall, and M. F. Schneider Activity-dependent and -independent nuclear fluxes of HDAC4 mediated by different kinases in adult skeletal muscle J. Cell Biol., March 14, 2005; 168(6): 887 - 897. [Abstract] [Full Text] [PDF]

				P. M. Garcia-Roves, T. E. Jones, K. Otani, D.-H. Han, and J. O. Holloszy Calcineurin Does Not Mediate Exercise-Induced Increase in Muscle GLUT4 Diabetes, March 1, 2005; 54(3): 624 - 628. [Abstract] [Full Text] [PDF]

				A. J Rose, B. J Michell, B. E Kemp, and M. Hargreaves Effect of exercise on protein kinase C activity and localization in human skeletal muscle J. Physiol., December 15, 2004; 561(3): 861 - 870. [Abstract] [Full Text] [PDF]

				T. Akimoto, T. J. Ribar, R. S. Williams, and Z. Yan Skeletal muscle adaptation in response to voluntary running in Ca2+/calmodulin-dependent protein kinase IV-deficient mice Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1311 - C1319. [Abstract] [Full Text] [PDF]

				S. L. McGee and M. Hargreaves Exercise and Myocyte Enhancer Factor 2 Regulation in Human Skeletal Muscle Diabetes, May 1, 2004; 53(5): 1208 - 1214. [Abstract] [Full Text] [PDF]