J Physiol Wellcome Trust-funded researchers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 553, Number 1, 65-81, November 15, 2003 DOI: 10.1113/jphysiol.2003.051631
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
553/1/65    most recent
jphysiol.2003.051631v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ling, S.
Right arrow Articles by Braun, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ling, S.
Right arrow Articles by Braun, A. P.
J Physiol (2003), 553.1, pp. 65-81
© Copyright 2003 D 2003 The Physiological Society
DOI: 10.1113/jphysiol.2003.051631

Syntaxin 1A co-associates with native rat brain and cloned large conductance, calcium-activated potassium channels in situ

Shizhang Ling, Jian-Zhong Sheng, Janice E. A. Braun* and Andrew P. Braun

Department of Pharmacology and Therapeutics, and *Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1

Large conductance, calcium-activated potassium channels (BKCa channels) are regulated by several distinct mechanisms, including phosphorylation/dephosphorylation events and protein-protein interactions. In this study, we have examined the interaction between BKCa channels and syntaxin 1A, a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that is reported to modulate the activity and/or localization of different classes of ion channels. Using a reciprocal co-immunoprecipitation strategy, we observed that native BKCa channels in rat hippocampus co-associate with syntaxin 1A, but not the closely related homologue syntaxin 3. This BKCa channel-syntaxin 1A interaction could be further demonstrated in a non-neuronal cell line (human embryonic kidney (HEK) 293 cells) following co-expression of rat syntaxin 1A and BKCa channels cloned from either mouse brain or bovine aorta. However, co-expression of these same channels with syntaxin 3 did not lead to a detectable protein-protein interaction. Immunofluorescent co-staining of HEK 293 cells expressing BKCa channels and syntaxin 1A demonstrated overlapping distribution of these two proteins in situ. Functionally, co-expression of BKCa channels with syntaxin 1A, but not syntaxin 3, was observed to enhance channel gating and kinetics at low concentrations (1-4 µM) of free cytosolic calcium, but not at higher concentrations (<= 10 µM), as judged by macroscopic current recordings in excised membrane patches. Interactions between BKCa channels and neighbouring membrane proteins may thus play important roles in regulating the activity and/or distribution of these channels within specialized cellular compartments.



This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. K. Isacson, Q. Lu, R. H. Karas, and D. H. Cox
RACK1 is a BKCa channel binding protein
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1459 - C1466.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. J. Loane, P. A. Lima, and N. V. Marrion
Co-assembly of N-type Ca2+ and BK channels underlies functional coupling in rat brain
J. Cell Sci., March 15, 2007; 120(6): 985 - 995.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. PsychiatryHome page
F. Laumonnier, S. Roger, P. Guerin, F. Molinari, R. M'Rad, D. Cahard, A. Belhadj, M. Halayem, A. M. Persico, M. Elia, et al.
Association of a Functional Deficit of the BKCa Channel, a Synaptic Regulator of Neuronal Excitability, With Autism and Mental Retardation
Am J Psychiatry, September 1, 2006; 163(9): 1622 - 1629.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Wareing, X. Bai, F. Seghier, C. M. Turner, S. L. Greenwood, P. N. Baker, M. J. Taggart, and G. K. Fyfe
Expression and function of potassium channels in the human placental vasculature
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R437 - R446.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. Lu, A. Alioua, Y. Kumar, M. Eghbali, E. Stefani, and L. Toro
MaxiK channel partners: physiological impact
J. Physiol., January 1, 2006; 570(1): 65 - 72.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. M. Cibulsky, H. Fei, and I. B. Levitan
Syntaxin-1A Binds to and Modulates the Slo Calcium-Activated Potassium Channel via an Interaction That Excludes Syntaxin Binding to Calcium Channels
J Neurophysiol, March 1, 2005; 93(3): 1393 - 1405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H.-H. Lim and C.-S. Park
Identification and Functional Characterization of Ankyrin-Repeat Family Protein ANKRA as a Protein Interacting with BKCa Channel
Mol. Biol. Cell, March 1, 2005; 16(3): 1013 - 1025.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Ling, J.-Z. Sheng, and A. P. Braun
The calcium-dependent activity of large-conductance, calcium-activated K+ channels is enhanced by Pyk2- and Hck-induced tyrosine phosphorylation
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C698 - C706.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
X.-P. Sun, B. Yazejian, and A. D. Grinnell
Electrophysiological properties of BK channels in Xenopus motor nerve terminals
J. Physiol., May 15, 2004; 557(1): 207 - 228.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 The Physiological Society.