HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 553/3/947    most recent jphysiol.2003.048462v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peters, D. Articles by Lieber, R. L. Search for Related Content PubMed PubMed Citation Articles by Peters, D. Articles by Lieber, R. L.

Asynchronous functional, cellular and transcriptional changes after a bout of eccentric exercise in the rat

David Peters, Ilona A. Barash, Michael Burdi, Philip S. Yuan, Liby Mathew, Jan Fridén* and Richard L. Lieber

Thirty eccentric contractions (ECs) were imposed upon rat dorsiflexors (n = 46) by activating the peroneal nerve and plantarflexing the foot ~40 deg, corresponding to a sarcomere length change over the range 2.27-2.39 µm for the tibialis anterior and 2.52-2.66 µm for the extensor digitorum longus. Animals were allowed to recover for one of 10 time periods ranging from 0.5 to 240 h, at which time muscle contractile properties, immunohistochemical labelling and gene expression were measured. Peak isometric torque dropped significantly by ~40 % from an initial level of 0.0530 ± 0.0009 Nm to 0.0298 ± 0.0008 Nm (P < 0.0001) immediately after EC, and then recovered in a linear fashion to control levels 168 h later. Immunohistochemical labelling of cellular proteins revealed a generally asynchronous sequence of events at the cellular level, with the earliest event measured being loss of immunostaining for the intermediate filament protein, desmin. Soon after the first signs of desmin loss, infiltration of inflammatory cells occurred, followed by a transient increase in membrane permeability, manifested as inclusion of plasma fibronectin. The quantitative polymerase chain reaction (QPCR) was used to measure transcript levels of desmin, vimentin, embryonic myosin heavy chain (MHC), myostatin, myoD and myogenin. Compared to control levels, myostatin transcripts were significantly elevated after only 0.5 h, myogenic regulatory factors significantly elevated after 3 h and desmin transcripts were significantly increased 12 h after EC. None of the measured parameters provide a mechanistic explanation for muscle force loss after EC. Future studies are required to investigate whether there is a causal relationship among desmin loss, increased cellular permeability, upregulation of the myoD and desmin genes, and, ultimately, an increase in the desmin content per sarcomere of the muscle.

This article has been cited by other articles:

				D. J. Mahoney, A. Safdar, G. Parise, S. Melov, M. Fu, L. MacNeil, J. Kaczor, E. T. Payne, and M. A. Tarnopolsky Gene expression profiling in human skeletal muscle during recovery from eccentric exercise Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1901 - R1910. [Abstract] [Full Text] [PDF]

				R. M. Crameri, P. Aagaard, K. Qvortrup, H. Langberg, J. Olesen, and M. Kjaer Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction J. Physiol., August 15, 2007; 583(1): 365 - 380. [Abstract] [Full Text] [PDF]

				I. A. Barash, M.-L. Bang, L. Mathew, M. L. Greaser, J. Chen, and R. L. Lieber Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle Am J Physiol Cell Physiol, July 1, 2007; 293(1): C218 - C227. [Abstract] [Full Text] [PDF]

				K. M. Heinemeier, J. L. Olesen, P. Schjerling, F. Haddad, H. Langberg, K. M. Baldwin, and M. Kjaer Short-term strength training and the expression of myostatin and IGF-I isoforms in rat muscle and tendon: differential effects of specific contraction types J Appl Physiol, February 1, 2007; 102(2): 573 - 581. [Abstract] [Full Text] [PDF]

				M. T. Woolstenhulme, R. K. Conlee, M. J. Drummond, A. W. Stites, and A. C. Parcell Temporal response of desmin and dystrophin proteins to progressive resistance exercise in human skeletal muscle J Appl Physiol, June 1, 2006; 100(6): 1876 - 1882. [Abstract] [Full Text] [PDF]

				K. J. B. Martins, T. Gordon, D. Pette, W. T. Dixon, G. R. Foxcroft, I. M. MacLean, and C. T. Putman Effect of satellite cell ablation on low-frequency-stimulated fast-to-slow fibre-type transitions in rat skeletal muscle J. Physiol., April 1, 2006; 572(1): 281 - 294. [Abstract] [Full Text] [PDF]

				S. P. Christiansen and L. K. McLoon The Effect of Resection on Satellite Cell Activity in Rabbit Extraocular Muscle Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 605 - 613. [Abstract] [Full Text] [PDF]

				E. R. Hentzen, M. Lahey, D. Peters, L. Mathew, I. A. Barash, J. Friden, and R. L. Lieber Stress-dependent and -independent expression of the myogenic regulatory factors and the MARP genes after eccentric contractions in rats J. Physiol., January 1, 2006; 570(1): 157 - 167. [Abstract] [Full Text] [PDF]

				J.-s. Kim, D. J. Kosek, J. K. Petrella, J. M. Cross, and M. M. Bamman Resting and load-induced levels of myogenic gene transcripts differ between older adults with demonstrable sarcopenia and young men and women J Appl Physiol, December 1, 2005; 99(6): 2149 - 2158. [Abstract] [Full Text] [PDF]

				T. A. Butterfield and W. Herzog Quantification of muscle fiber strain during in vivo repetitive stretch-shortening cycles J Appl Physiol, August 1, 2005; 99(2): 593 - 602. [Abstract] [Full Text] [PDF]

				C. I. Martin and I. A. Johnston The role of myostatin and the calcineurin-signalling pathway in regulating muscle mass in response to exercise training in the rainbow trout Oncorhynchus mykiss Walbaum J. Exp. Biol., June 1, 2005; 208(11): 2083 - 2090. [Abstract] [Full Text] [PDF]

				M. B. Reid Response of the ubiquitin-proteasome pathway to changes in muscle activity Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1423 - R1431. [Abstract] [Full Text] [PDF]

				Y. Yang, A. Creer, B. Jemiolo, and S. Trappe Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle J Appl Physiol, May 1, 2005; 98(5): 1745 - 1752. [Abstract] [Full Text] [PDF]

				F. X Pizza, J. M Peterson, J. H Baas, and T. J Koh Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice J. Physiol., February 1, 2005; 562(3): 899 - 913. [Abstract] [Full Text] [PDF]

				M. M. Bamman, R. C. Ragan, J.-s. Kim, J. M. Cross, V. J. Hill, S. C. Tuggle, and R. M. Allman Myogenic protein expression before and after resistance loading in 26- and 64-yr-old men and women J Appl Physiol, October 1, 2004; 97(4): 1329 - 1337. [Abstract] [Full Text] [PDF]

				R. M. Crameri, H. Langberg, P. Magnusson, C. H. Jensen, H. D. Schroder, J. L. Olesen, C. Suetta, B. Teisner, and M. Kjaer Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise J. Physiol., July 1, 2004; 558(1): 333 - 340. [Abstract] [Full Text] [PDF]

				I. A. Barash, L. Mathew, A. F. Ryan, J. Chen, and R. L. Lieber Rapid muscle-specific gene expression changes after a single bout of eccentric contractions in the mouse Am J Physiol Cell Physiol, February 1, 2004; 286(2): C355 - C364. [Abstract] [Full Text]