| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Departments of 1 Medical Pharmacology and Physiology 2 Biomedical Sciences 3 Biochemistry, University of Missouri, Columbia, MO 65212, USA 4 Departments of Internal Medicine and Urology, University of Michigan, Ann Arbor, MI 48109, USA
It is well established that after metastatic cancer cells escape the primary tumour and enter the circulation, their interactions with microvascular endothelium of a target organ constitute an essential rate-limiting step in haematogenous cancer metastasis. However, the physiological and biochemical processes supporting neoplastic cell arrest and retention in the microcirculation are still poorly understood. In this study, we present experimental evidence that microvascular endothelium of metastasis-prone tissues undergoes activation in response to desialylated cancer-associated carbohydrate structures such as Thomsen–Friedenreich (TF) antigen (Galß1–3GalNAc) expressed on circulating glycoproteins and neoplastic cells. The metastasis-associated endothelium activation, manifested by marked increase in endothelial cell surface galectin-3 expression, causes gradual decrease in cancer cell velocities (from 72 x 102± 33 x 102µm s-1 to 7.6 x 102± 1.9 x 102µm s-1, mean ±S.D.) accompanied by a corresponding increase in the percentage of rolling cells (from 3.3%± 1.2% to 24.3%± 3.6%, mean ±S.D.), and results in human breast and prostate carcinoma cell arrest and retention in the microvasculature. This process, which could be of high importance in haematogenous cancer metastasis, was inhibited efficiently by an anti-TF antigen function-blocking antibody. Carbohydrate-mediated endothelial activation could be a process of physiological significance as it probably occurs in the interactions between a variety of circulating constituents and the vessel wall.
(Received 9 September 2003;
accepted after revision 13 October 2003;
first published online 17 October 2003)
Corresponding author V. Glinsky: Department of Biochemistry, University of Missouri, M743 Medical Sciences Bldg, Columbia, MO 65212, USA. Email: glinskiivl{at}missouri.edu
This article has been cited by other articles:
|
|
 |
|
  S. R. Kumar and S. L. Deutscher 111In-Labeled Galectin-3-Targeting Peptide as a SPECT Agent for Imaging Breast Tumors J. Nucl. Med., May 1, 2008; 49(5): 796 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. L. Thijssen, S. Hulsmans, and A. W. Griffioen The Galectin Profile of the Endothelium: Altered Expression and Localization in Activated and Tumor Endothelial Cells Am. J. Pathol., February 1, 2008; 172(2): 545 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. L. J. L. Thijssen, F. Poirier, L. G. Baum, and A. W. Griffioen Galectins in the tumor endothelium: opportunities for combined cancer therapy Blood, October 15, 2007; 110(8): 2819 - 2827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Glinskii, T. W. Abraha, J. R. Turk, L. J. Rubin, V. H. Huxley, and V. V. Glinsky Microvascular network remodeling in dura mater of ovariectomized pigs: role for angiopoietin-1 in estrogen-dependent control of vascular stability Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1131 - H1137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schluter, P. Gassmann, A. Enns, T. Korb, A. Hemping-Bovenkerk, J. Holzen, and J. Haier Organ-Specific Metastatic Tumor Cell Adhesion and Extravasation of Colon Carcinoma Cells with Different Metastatic Potential Am. J. Pathol., September 1, 2006; 169(3): 1064 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zou, V. V. Glinsky, L. A. Landon, L. Matthews, and S. L. Deutscher Peptides specific to the galectin-3 carbohydrate recognition domain inhibit metastasis-associated cancer cell adhesion Carcinogenesis, February 1, 2005; 26(2): 309 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Dimitroff, M. Lechpammer, D. Long-Woodward, and J. L. Kutok Rolling of Human Bone-Metastatic Prostate Tumor Cells on Human Bone Marrow Endothelium under Shear Flow Is Mediated by E-Selectin Cancer Res., August 1, 2004; 64(15): 5261 - 5269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. I. Romanov, T. Whyard, H. L. Adler, W. C. Waltzer, and S. Zucker Prostate Cancer Cell Adhesion to Bone Marrow Endothelium: The Role of Prostate-Specific Antigen Cancer Res., March 15, 2004; 64(6): 2083 - 2089. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
