| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SYMPOSIUM REPORT |
1 Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR, USA 2 Vollum Institute, Oregon Health and Science University, Portland, OR, USA
Small conductance Ca2+-activated K+ channels (SK channels) contribute to the long lasting afterhyperpolarization (AHP) that follows an action potential in many central neurones. The biophysical and pharmacological attributes of cloned SK channels strongly suggest that one or more of them underlie the medium component of the AHP that regulates interspike interval and plays an important role in setting tonic firing frequency. The cloned SK channels comprise a distinct subfamily of K+ channels. Heterologously expressed SK channels recapitulate the biophysical and pharmacological hallmarks of native SK channels, being gated solely by intracellular Ca2+ ions with no voltage dependence to their gating, small unitary conductance values and sensitivity to the bee venom peptide toxin, apamin. Molecular, biochemical and electrophysiological studies have revealed that Ca2+ gating in SK channels is due to heteromeric assembly of the SK 
pore-forming subunits with calmodulin (CaM). Ca2+ binding to the N-terminal E–F hands of CaM is responsible for SK channel gating. Crystallographic studies suggest that SK channels gate as a dimer-of-dimers, and that the physical gate of SK channels resides at or near the selectivity filter of the channels. In addition, Ca2+-independent interactions between the SK channel subunits and CaM are necessary for proper membrane trafficking.
(Received 11 June 2003;
accepted after revision 18 September 2003;
first published online 18 September 2003)
Corresponding author J. P. Adelman: Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA. Email: adelman{at}ohsu.edu
This article has been cited by other articles:
|
|
 |
|
  M. Bal, O. Zaika, P. Martin, and M. S. Shapiro Calmodulin binding to M-type K+ channels assayed by TIRF/FRET in living cells J. Physiol., May 1, 2008; 586(9): 2307 - 2320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ramanathan, T. Tkatch, J. F. Atherton, C. J. Wilson, and M. D. Bevan D2-Like Dopamine Receptors Modulate SKCa Channel Function in Subthalamic Nucleus Neurons Through Inhibition of Cav2.2 Channels J Neurophysiol, February 1, 2008; 99(2): 442 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Nishimura, K. Ui-Tei, K. Saigo, H. Ishii, Y. Sakuma, and M. Kato 17{beta}-Estradiol at Physiological Concentrations Augments Ca2+-Activated K+ Currents via Estrogen Receptor {beta} in the Gonadotropin-Releasing Hormone Neuronal Cell Line GT1-7 Endocrinology, February 1, 2008; 149(2): 774 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Zaika, G. P. Tolstykh, D. B. Jaffe, and M. S. Shapiro Inositol Triphosphate-Mediated Ca2+ Signals Direct Purinergic P2Y Receptor Regulation of Neuronal Ion Channels J. Neurosci., August 15, 2007; 27(33): 8914 - 8926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Willoughby and D. M. F. Cooper Organization and Ca2+ Regulation of Adenylyl Cyclases in cAMP Microdomains Physiol Rev, July 1, 2007; 87(3): 965 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lu, Q. Zhang, V. Timofeyev, Z. Zhang, J. N. Young, H.-S. Shin, A. A. Knowlton, and N. Chiamvimonvat Molecular Coupling of a Ca2+-Activated K+ Channel to L-Type Ca2+ Channels via {alpha}-Actinin2 Circ. Res., January 5, 2007; 100(1): 112 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Waring and J. L. Turgeon Estradiol Inhibition of Voltage-Activated and Gonadotropin-Releasing Hormone-Induced Currents in Mouse Gonadotrophs Endocrinology, December 1, 2006; 147(12): 5798 - 5805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Srivastava, K. Ko, P. Choudhury, Z. Li, A. K. Johnson, V. Nadkarni, D. Unutmaz, W. A. Coetzee, and E. Y. Skolnik Phosphatidylinositol-3 Phosphatase Myotubularin-Related Protein 6 Negatively Regulates CD4 T Cells Mol. Cell. Biol., August 1, 2006; 26(15): 5595 - 5602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Srivastava, P. Choudhury, Z. Li, G. Liu, V. Nadkarni, K. Ko, W. A. Coetzee, and E. Y. Skolnik Phosphatidylinositol 3-Phosphate Indirectly Activates KCa3.1 via 14 Amino Acids in the Carboxy Terminus of KCa3.1 Mol. Biol. Cell, January 1, 2006; 17(1): 146 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wesselius, D. J. Heersema, J. P. Mostert, M. Heerings, F. Admiraal-Behloul, A. Talebian, M. A. van Buchem, and J. De Keyser A randomized crossover study of bee sting therapy for multiple sclerosis Neurology, December 13, 2005; 65(11): 1764 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shin, H. Soh, S. Chang, D. H. Kim, and C.-S. Park Sodium Permeability of a Cloned Small-Conductance Calcium-Activated Potassium Channel Biophys. J., November 1, 2005; 89(5): 3111 - 3119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Teruyama and W. E Armstrong Enhancement of calcium-dependent afterpotentials in oxytocin neurons of the rat supraoptic nucleus during lactation J. Physiol., July 15, 2005; 566(2): 505 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Strassmaier, C. T. Bond, C. A. Sailer, H.-G. Knaus, J. Maylie, and J. P. Adelman A Novel Isoform of SK2 Assembles with Other SK Subunits in Mouse Brain J. Biol. Chem., June 3, 2005; 280(22): 21231 - 21236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. F. Lee, J. C. Callaway, and R. C. Foehring Effects of Temperature on Calcium Transients and Ca2+-Dependent Afterhyperpolarizations in Neocortical Pyramidal Neurons J Neurophysiol, April 1, 2005; 93(4): 2012 - 2020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chemin, A. Patel, F. Duprat, M. Zanzouri, M. Lazdunski, and E. Honore Lysophosphatidic Acid-operated K+ Channels J. Biol. Chem., February 11, 2005; 280(6): 4415 - 4421. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
