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1 CURE: Digestive Diseases Research Center and Center for Neurovisceral Sciences, VA Greater Los Angeles Healthcare System, Department of Medicine, Digestive Diseases Division and Brain Research Institute, University of California at Los Angeles, Los Angeles, CA 90073, USA2 The Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA 92037-1099, USA3 Neurocrine Biosciences Inc., La Jolla, CA, 92121-1102, USA
Recently characterized selective agonists and developed antagonists for the corticotropin releasing factor (CRF) receptors are new tools to investigate stress-related functional changes. The influence of mammalian CRF and related peptides injected intracerebroventricularly (I.C.V.) on gastric and colonic motility, and the CRF receptor subtypes involved and their role in colonic response to stress were studied in conscious mice. The CRF1/CRF2 agonists rat urocortin 1 (rUcn 1) and rat/human CRF (r/h CRF), the preferential CRF1 agonist ovine CRF (oCRF), and the CRF2 agonist mouse (m) Ucn 2, injected I.C.V. inhibited gastric emptying and stimulated distal colonic motor function (bead transit and defecation) while oCRF9–33OH (devoid of CRF receptor affinity) showed neither effects. mUcn 2 injected peripherally had no colonic effect. The selective CRF2 antagonist astressin2-B (I.C.V.), at a 20 : 1 antagonist: agonist ratio, blocked I.C.V. r/hCRF and rUcn 1 induced inhibition of gastric transit and reduced that of mUcn 2, while the CRF1 antagonist NBI-35965 had no effect. By contrast, the colonic motor stimulation induced by I.C.V. r/hCRF and rUcn 1 and 1h restraint stress were antagonized only by NBI-35965 while stimulation induced by mUcn 2 was equally blocked by both antagonists. None of the CRF antagonists injected I.C.V. alone influenced gut transit. These data establish in mice that brain CRF1 receptors mediate the stimulation of colonic transit induced by central CRF, urocortins (1 and 2) and restraint stress, while CRF2 receptors mediate the inhibitory actions of these peptides on gastric transit.
(Received 13 December 2003;
accepted after revision 26 January 2004;
first published online 30 January 2004)
Corresponding author Y. Taché: CURE: Digestive Diseases Research Center and Center for Neurovisceral Sciences, VA Greater Los Angeles Healthcare System, Bldg 115, Rm 117, 11301 Whilshire Blvd., Los Angeles CA 90073, USA. Email: ytache{at}ucla.edu
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