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J Physiol Volume 556, Number 2, 623-635, April 15, 2004 DOI: 10.1113/jphysiol.2003.058727
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Rat transcobalamin: cloning and regulation of mRNA expression

Seema Kalra1, Shakuntla Seetharam1, Raghunatha R. Yammani1 and Bellur Seetharam12

Departments of 1 Medicine2 Biochemistry, Division of Gastroenterology and Hepatology, Medical College of Wisconsin and Clement Zablocki VA Medical Center, Milwaukee, WI 53295, USA

Transcobalamin (TC) has been cloned and used for studying its gene expression in the rat. TC mRNA is distributed widely in adult rat tissues, but at different levels (kidney > liver > lung > yolk sac > intestine > heart > brain > spleen > muscle). TC mRNA levels were 4-fold higher in the jejunum and ileum compared to its levels in the duodenum. During postnatal development, TC mRNA levels in the ileum declined 4-fold from day 4 to day 12, but increased by 5-fold between days 12 and 24. In contrast, TC mRNA levels increased by 2.5-fold in the kidney from day 4 to day 12 and then declined by 2-fold by day 24. Adrenalectomy of adult rats resulted in a 4-fold decline in ileal levels of TC mRNA and a 50% decline in the ileal mucosal formation of the TC-[57Co] cobalamin (Cbl) complex following oral administration of [57Co]Cbl complexed to gastric intrinsic factor (IF). Cortisone treatment reversed these changes noted in the ileum. In contrast to ileum, kidney TC mRNA levels were not altered significantly in adrenalectomized rats before and after cortisone treatment. Taken together, this study has provided evidence for the regulation of TC gene expression in the rat kidney and intestine during their postnatal development, and cortisone selectively regulates ileal but not kidney TC mRNA levels.

(Received 25 November 2003; accepted after revision 13 January 2004; first published online 14 January 2004)
Corresponding author B. Seetharam: VA Medical Center, Research 151, 5000 West National Avenue, Milwaukee, WI 53295, USA. Email: seethara{at}mcw.edu






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