| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Physiology and Biophysics/C-240, University of Colorado Medical School, 4200 East Ninth Avenue, Denver, CO 80262, USA
Phosphoinositides are key regulators of synaptic vesicle cycling and endocytic traffic; the actin cytoskeleton also seems to be involved in modulating these processes. We investigated the effects of perturbing phosphoinositide signalling and actin dynamics on vesicle cycling in frog motor nerve terminals, using fluorescence and electron microscopy, and electrophysiology. Antibody staining for ß-actin revealed that actin surrounds but does not overlap with synaptic vesicle clusters. Latrunculin A, which disrupts actin filaments by binding actin monomers, and wortmannin, an inhibitor of phosphatidyl inositol-3-kinase (PI3-kinase), each disrupted the pattern of presynaptic actin staining, but not vesicle clusters in resting terminals. Latrunculin A, but not wortmannin, also reduced vesicle mobilization and exocytosis. Both drugs inhibited the stimulation-induced uptake of the styryl dye FM1-43 and blocked vesicle reformation from internalized membrane objects after tetanic stimulation. These results are consistent with a role of PI3-kinase and the actin cytoskeleton in the slow pathway of vesicle endocytosis, used primarily by reserve pool vesicles.
(Received 30 January 2004;
accepted after revision 3 March 2004;
first published online 5 March 2004)
Corresponding author W. J. Betz: Department of Physiology and Biophysics/C-240, University of Colorado Medical School, 4200 East Ninth Avenue, Denver, CO 80262, USA. Email: bill.betz{at}uchsc.edu
This article has been cited by other articles:
|
|
 |
|
  N. Vijayakrishnan, E. A. Woodruff III, and K. Broadie Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses J. Cell Sci., January 1, 2009; 122(1): 114 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Andersson, J. Jakobsson, P. Low, O. Shupliakov, and L. Brodin Perturbation of Syndapin/PACSIN Impairs Synaptic Vesicle Recycling Evoked by Intense Stimulation J. Neurosci., April 9, 2008; 28(15): 3925 - 3933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Lee and H. B. Peng The Function of Mitochondria in Presynaptic Development at the Neuromuscular Junction Mol. Biol. Cell, January 1, 2008; 19(1): 150 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Clayton, G. J. O. Evans, and M. A. Cousin Activity-dependent control of bulk endocytosis by protein dephosphorylation in central nerve terminals J. Physiol., December 15, 2007; 585(3): 687 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Gaffield and W. J. Betz Synaptic Vesicle Mobility in Mouse Motor Nerve Terminals with and without Synapsin J. Neurosci., December 12, 2007; 27(50): 13691 - 13700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kreda, S. F. Okada, C. A. van Heusden, W. O'Neal, S. Gabriel, L. Abdullah, C. W. Davis, R. C. Boucher, and E. R. Lazarowski Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells J. Physiol., October 1, 2007; 584(1): 245 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. O. Evans and M. A. Cousin Activity-Dependent Control of Slow Synaptic Vesicle Endocytosis by Cyclin-Dependent Kinase 5 J. Neurosci., January 10, 2007; 27(2): 401 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tokuoka and Y. Goda Myosin Light Chain Kinase Is Not a Regulator of Synaptic Vesicle Trafficking during Repetitive Exocytosis in Cultured Hippocampal Neurons. J. Neurosci., November 8, 2006; 26(45): 11606 - 11614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nunes, N. Haines, V. Kuppuswamy, D. J. Fleet, and B. A. Stewart Synaptic Vesicle Mobility and Presynaptic F-Actin Are Disrupted in a N-ethylmaleimide-sensitive Factor Allele of Drosophila Mol. Biol. Cell, November 1, 2006; 17(11): 4709 - 4719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yao, J. Qi, and G. Chen Actin-Dependent Activation of Presynaptic Silent Synapses Contributes to Long-Term Synaptic Plasticity in Developing Hippocampal Neurons J. Neurosci., August 2, 2006; 26(31): 8137 - 8147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Ashton and Y. A. Ushkaryov Properties of Synaptic Vesicle Pools in Mature Central Nerve Terminals J. Biol. Chem., November 4, 2005; 280(44): 37278 - 37288. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
