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J Physiol Volume 558, Number 3, 745-757, August 1, 2004 DOI: 10.1113/jphysiol.2004.066845
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P2X receptor subtype-specific modulation of excitatory and inhibitory synaptic inputs in the rat brainstem

Tomokazu Watano, Jennifer A. Calvert, Catherine Vial, Ian D. Forsythe and Richard J. Evans

Department of Cell Physiology and Pharmacology, University of Leicester, PO Box 138, University Road, Leicester, LE1 9HN, UK

The role of P2 receptors in synaptic transmission to the rat medial nucleus of the trapezoid body (MNTB) was studied in an in vitro brain slice preparation. Whole-cell patch recordings were made and spontaneous synaptic responses studied under voltage clamp during application of P2X receptor agonists. ATP{gamma}S (100 µM) had no effect on holding current, but facilitated spontaneous excitatory postsynaptic current (sEPSC) frequency in 41% of recordings and facilitated spontaneous inhibitory postsynaptic currents (sIPSCs) in 20% of recordings. These were blocked by the P2 receptor antagonist suramin (100 µM). {alpha},ß-meATP also facilitated sEPSC and sIPSC frequency, while L-ß,{gamma}-meATP facilitated only sIPSCs. The sEPSC facilitation by ATP{gamma}S was blocked by TTX (but did not block facilitation of sIPSCs). sEPSC facilitation was blocked by PPADS (30 µM) and the selective P2X3 receptor antagonist A-317491 (3 µM), suggesting that modulation of sEPSCs involves P2X3 receptor subunits. {alpha},ß-meATP-facilitated sIPSCs were also recorded in wild-type mouse MNTB neurones, but were absent in the MNTB from P2X1 receptor-deficient mice demonstrating a functional role for P2X1 receptors in the CNS.

(Received 21 April 2004; accepted after revision 31 May 2004; first published online 4 June 2004)
Corresponding author R. J. Evans: Department of Cell Physiology and Pharmacology, University of Leicester, PO Box 138, University Road, Leicester, LE1 9HN, UK. Email: RJE6{at}le.ac.uk




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