| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1
In aquatic vertebrates, hypoxia induces physiological changes that arise principally from O2 chemoreceptors of the gill. Neuroepithelial cells (NECs) of the zebrafish gill are morphologically similar to mammalian O2 chemoreceptors (e.g. carotid body), suggesting that they may play a role in initiating the hypoxia response in fish. We describe morphological changes of zebrafish gill NECs following in vivo exposure to chronic hypoxia, and characterize the cellular mechanisms of O2 sensing in isolated NECs using patch-clamp electrophysiology. Confocal immunofluorescence studies indicated that chronic hypoxia (PO2 = 35 mmHg, 60 days) induced hypertrophy, proliferation and process extension in NECs immunoreactive for serotonin or synaptic vesicle protein (SV2). Under voltage clamp, NECs responded to hypoxia (PO2 = 25–140 mmHg) with a dose-dependent decrease in K+ current. The current–voltage relationship of the O2-sensitive current (IKO2) reversed near EK and displayed open rectification. Pharmacological characterization indicated that IKO2 was resistant to 20 mM tetraethylammonium (TEA) and 5 mM 4-aminopyridine (4-AP), but was sensitive to 1 mM quinidine. In current-clamp recordings, hypoxia produced membrane depolarization associated with a conductance decrease; this depolarization was blocked by quinidine, but was insensitive to TEA and 4-AP. These biophysical and pharmacological characteristics suggest that hypoxia sensing in zebrafish gill NECs is mediated by inhibition of a background K+ conductance, which generates a receptor potential necessary for neurosecretion and activation of sensory pathways in the gill. This appears to be a fundamental mechanism of O2 sensing that arose early in vertebrate evolution, and was adopted later in mammalian O2 chemoreceptors.
(Received 1 June 2004;
accepted after revision 24 August 2004;
first published online 26 August 2004)
Corresponding author C. A. Nurse: Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1. Email: nursec{at}mcmaster.ca
This article has been cited by other articles:
|
|
 |
|
  K. R. Olson Hydrogen sulfide and oxygen sensing: implications in cardiorespiratory control J. Exp. Biol., September 1, 2008; 211(17): 2727 - 2734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Jonz and C. A. Nurse New developments on gill innervation: insights from a model vertebrate J. Exp. Biol., August 1, 2008; 211(15): 2371 - 2378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Olson, M. J. Healy, Z. Qin, N. Skovgaard, B. Vulesevic, D. W. Duff, N. L. Whitfield, G. Yang, R. Wang, and S. F. Perry Hydrogen sulfide as an oxygen sensor in trout gill chemoreceptors Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R669 - R680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Coolidge, C. S. Ciuhandu, and W. K. Milsom A comparative analysis of putative oxygen-sensing cells in the fish gill J. Exp. Biol., April 15, 2008; 211(8): 1231 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sundin, M. L. Burleson, A. P. Sanchez, J. Amin-Naves, R. Kinkead, L. H. Gargaglioni, L. K. Hartzler, M. Wiemann, P. Kumar, and M. L. Glass Respiratory chemoreceptor function in vertebrates comparative and evolutionary aspects Integr. Comp. Biol., October 1, 2007; 47(4): 592 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Evans AMP-activated protein kinase and the regulation of Ca2+ signalling in O2-sensing cells J. Physiol., July 1, 2006; 574(1): 113 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Vulesevic, B. McNeill, and S. F. Perry Chemoreceptor plasticity and respiratory acclimation in the zebrafish Danio rerio J. Exp. Biol., April 1, 2006; 209(7): 1261 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Jonz and C. A. Nurse Development of oxygen sensing in the gills of zebrafish J. Exp. Biol., April 15, 2005; 208(8): 1537 - 1549. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
