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¹ Department of Pharmacology, Physiology and Neuroscience, University of South Carolina School of Medicine, Columbia, SC 29208, USA

The GABA_A receptors show a large degree of structural heterogeneity, with seven different subunit families, and 16 different subtypes in mammalian species. The family is the largest, with six different subtypes. The 1 and 6 subtypes are among the most diverse within this family and confer distinct pharmacological properties to recombinant and neuronal receptors. To determine whether different single channel and macroscopic kinetic properties were also associated with these subtypes, the 1 or 6 subunit was expressed in mammalian cells along with ß3 and 2L subunits and the kinetic properties examined with outside-out patch recordings. The 1ß32L receptors responded to GABA with long-duration openings organized into multi-opening bursts. In contrast, channel openings of the 6ß32L receptors were predominately short in duration and occurred as isolated, single openings. The subunit subtype also affected the deactivation rate of the receptor, which was almost 2-fold slower for 6ß32L, compared with the 1ß32L isoform. Onset of fast desensitization did not differ between the isoforms. To determine the structural domains responsible for these differences in kinetic properties, we constructed six chimeric subunits, combining different regions of the 1 and 6 subunits. The properties of the chimeric subunits indicated that structures within the third transmembrane domain (TM3) and the TM3–TM4 intracellular loop conferred differences in single channel gating kinetics that subsequently affected the deactivation rate and GABA EC₅₀. The effect of agonist concentration on the rise time of the current showed that the extracellular N-terminal domain was largely responsible for binding characteristics, while the transmembrane domains determined the activation rate at saturating GABA concentrations. This suggests that subunit structures outside of the agonist binding and pore-lining domains are responsible for the kinetic differences conferred by the 1 and 6 subtypes. Structural heterogeneity within these transmembrane and intracellular regions can therefore influence the characteristics of the postsynaptic response of GABA_A receptors with different subunit composition.

(Received 1 June 2004; accepted after revision 5 October 2004; first published online 7 October 2004)
Corresponding author J. L. Fisher: Department of Pharmacology, Physiology and Neuroscience, University of South Carolina School of Medicine, Columbia, SC 29208, USA. Email: jfisher{at}gw.med.sc.edu

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