J Physiol Society Membership
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 562, Number 1, 235-244, January 1, 2005 DOI: 10.1113/jphysiol.2004.071266
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
562/1/235    most recent
jphysiol.2004.071266v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duprat, F.
Right arrow Articles by Lazdunski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duprat, F.
Right arrow Articles by Lazdunski, M.

Pancreatic two P domain K+ channels TALK-1 and TALK-2 are activated by nitric oxide and reactive oxygen species

F. Duprat1, C. Girard1, G. Jarretou1 and M. Lazdunski1

1 Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR 6097, 660 route des Lucioles, Sophia Antipolis, 06560 Vabonne, France

This study firstly shows with in situ hybridization on human pancreas that TALK-1 and TALK-2, two members of the 2P domain potassium channel (K2P) family, are highly and specifically expressed in the exocrine pancreas and absent in Langherans islets. On the contrary, expression of TASK-2 in mouse pancreas is found both in the exocrine pancreas and in the Langherans islets. This study also shows that TALK-1 and TALK-2 channels, expressed in Xenopus oocytes, are strongly and specifically activated by nitric oxide (obtained with a mixture of sodium nitroprussate (SNP) and dithiothreitol (DTT)), superoxide anion (obtained with xanthine and xanthine oxidase) and singlet oxygen (obtained upon photoactivation of rose bengal, and with chloramine T). Other nitric oxide and reactive oxygen species (NOS and ROS) donors, as well as reducing conditions were found to be ineffective on TALK-1, TALK-2 and TASK-2 (sin-1, angeli's salt, SNP alone, tBHP, H2O2, and DTT). These results suggest that, in the exocrine pancreas, specific members of the NOS and ROS families could act as endogenous modulators of TALK channels with a role in normal secretion as well as in disease states such as acute pancreatitis and apoptosis.

(Received 5 July 2004; accepted after revision 21 October 2004; first published online 28 October 2004)
Corresponding author M. Lazdunski: Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR 6097, 660 route des Lucioles, Sophia Antipolis, 06560 Vabonne, France. Email: ipmc{at}ipmc.cnrs.fr




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
D. Heitzmann and R. Warth
Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia
Physiol Rev, July 1, 2008; 88(3): 1119 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. Toyoda, M. Saito, H. Sato, Y. Dempo, A. Ohashi, T. Hirai, Y. Maeda, T. Kaneko, and Y. Kang
cGMP Activates a pH-Sensitive Leak K+ Current in the Presumed Cholinergic Neuron of Basal Forebrain
J Neurophysiol, May 1, 2008; 99(5): 2126 - 2133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L'Hoste, M. Poet, C. Duranton, R. Belfodil, H. e Barriere, I. Rubera, M. Tauc, C. Poujeol, J. Barhanin, and P. Poujeol
Role of TASK2 in the Control of Apoptotic Volume Decrease in Proximal Kidney Cells
J. Biol. Chem., December 14, 2007; 282(50): 36692 - 36703.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 The Physiological Society.