| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology, University of Utah, 410 Chipeta Way, Salt Lake City, UT 84108-1297, USA
The electrical properties of sustentacular cells (SCs) in the olfactory epithelium (OE) were investigated in tissue slices taken from neonatal mice (P0–P4). Conventional whole-cell recordings were obtained from SCs and also from olfactory receptor neurones (ORNs) in
situ. SCs had a larger apparent cell capacitance (Ccell) (18.6 ± 0.5 pF) than ORNs (4.4 ± 0.4 pF) and a lower apparent membrane resistance (Rm) (160 ± 11 M
versus 664 ± 195 M, respectively). When corrected for a seal resistance of 1 G, these mean Rm values were increased to 190 M and 2 G in SCs and ORNs, respectively. SCs generated a TTX (1 µM)-resistant voltage-activated Na+ current (INa) that had a peak density at –38 mV of –44 pA pF–1 and supported action potential firing. Peak current density of INa in neurones was 510 ± 96 pA pF–1. The outward K+ current in SCs was composed (> 70%) of a TEA (2 mM)-sensitive component that was mediated by the opening of large-conductance (237 ± 10 pS; BK) channels. The resting leak conductance (gL) of SCs was permeable to monovalent cations and anions and was largely inhibited by substitution of external Na+ with NMDG and by internal F– with gluconate. gL deactivated up to 50% at potentials negative of –70 mV and was inhibited by 18ß-glycyrrhetinic acid (20 µM). SCs were identified using fluorescent dyes (Lucifer Yellow and Alexa Fluor 488) in the whole-cell patch pipette-filling solution. Our findings indicate that SCs in the OE of neonates are electrically excitable and are distinguishable from neurones by a having a resting gL.
(Received 12 November 2004;
accepted after revision 3 December 2004;
first published online 20 December 2004)
Corresponding author M. T. Lucero: 410 Chipeta Way, Room 156, University of Utah, Salt Lake City, UT 84108-1297, USA. Email: mary.lucero{at}m.cc.utah.edu
This article has been cited by other articles:
|
|
 |
|
  T. G. Paunescu, A. C. Jones, R. Tyszkowski, and D. Brown V-ATPase expression in the mouse olfactory epithelium Am J Physiol Cell Physiol, October 1, 2008; 295(4): C923 - C930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Grubb, T. D. Rogers, H. M. Kulaga, K. A. Burns, R. L. Wonsetler, R. R. Reed, and L. E. Ostrowski Olfactory epithelia exhibit progressive functional and morphological defects in CF mice Am J Physiol Cell Physiol, August 1, 2007; 293(2): C574 - C583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Wine The inexhaustible mouse nose. Focus on "Olfactory epithelia exhibit progressive functional and morphological defects in CF mice" Am J Physiol Cell Physiol, August 1, 2007; 293(2): C537 - C539. [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shimazaki, A. Boccaccio, A. Mazzatenta, G. Pinato, M. Migliore, and A. Menini Electrophysiological Properties and Modeling of Murine Vomeronasal Sensory Neurons in Acute Slice Preparations Chem Senses, June 1, 2006; 31(5): 425 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Nickell, N. K. Kleene, R. C. Gesteland, and S. J. Kleene Neuronal Chloride Accumulation in Olfactory Epithelium of Mice Lacking NKCC1 J Neurophysiol, March 1, 2006; 95(3): 2003 - 2006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vogalis, C. C. Hegg, and M. T. Lucero Electrical Coupling in Sustentacular Cells of the Mouse Olfactory Epithelium J Neurophysiol, August 1, 2005; 94(2): 1001 - 1012. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
