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This Article Full Text Full Text (PDF) All Versions of this Article: 563/1/213    most recent jphysiol.2003.056531v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roberts, J. L Articles by Clark, M. G Search for Related Content PubMed PubMed Citation Articles by Roberts, J. L Articles by Clark, M. G

¹ Tasmanian Partnership for Advanced Computing, University of Tasmania, Private Bag 37, Hobart 7001, Tasmania, Australia
² Biochemistry, Medical School, University of Tasmania, Private Bag 58, Hobart 7001, Tasmania, Australia
³ Department of the Environment and Heritage, Australian Antarctic Division, Private Bag 80, Hobart 7001, Tasmania, Australia

Theoretical models for the description of microdialysis outflow:inflow (O/I) ratio for ³H₂O and [¹⁴C]ethanol were developed, taking into account the nutritive fraction of total blood flow in muscle. The models yielded an approximately exponential decay expression for the O/I ratio, dependent on the physical dimensions of a linear probe (length and radius), the flow rate through the probe, muscle blood flow (including the nutritive fraction) and the diffusion coefficients for the tracer in the probe and muscle. The models compared favourably with experimental data from the constant-flow perfused rat hindlimb. Estimates of the nutritive fraction of total blood flow from experimental data were determined by minimizing the error between model and experimental data. The nutritive fraction was found to be 0.22 ± 0.04 under basal perfusion conditions. When 70 nM noradrenaline (norepinephrine) was included in the perfusion medium, the nutritive fraction was 0.91 ± 0.06 (P < 0.05). The inclusion of 300 nM serotonin, decreased the nutritive fraction to 0.05 ± 0.01 (P < 0.05). This model can be applied to the determination of nutritive fraction of skeletal muscle blood flow in physiologically relevant microvascular conditions such as during exercise and in disease states.

(Received 31 August 2004; accepted after revision 14 December 2004; first published online 20 December 2004)
Corresponding author J. M. B. Newman: Biochemistry, Medical School, University of Tasmania, Private Bag 58, Hobart 7001, Tasmania, Australia. Email: j.newman{at}utas.edu.au

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