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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 563/3/663    most recent jphysiol.2004.079558v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nimmrich, V. Articles by Draguhn, A. Search for Related Content PubMed PubMed Citation Articles by Nimmrich, V. Articles by Draguhn, A.

¹ Abbott GmbH & Co. KG, Neuroscience Research, Knollstrasse 50, 67061 Ludwigshafen, Germany
² Neuroscience Research Centre at the Charité, Charité University Hospital, Schumannstrasse 20/21, 10117 Berlin, Germany
³ Institute of Physiology and Pathophysiology, Heidelberg University, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany

The characteristic, behaviour-related network oscillations of the mammalian hippocampus (, and ripples) are accompanied by strongly phase-coupled action potentials in specific subsets of GABAergic interneurones. It has been suggested that the resulting phasic, repetitive inhibition shapes rhythmic coherent activity of the neuronal network. Here, we examined whether synaptic inhibition entrains 200 Hz network ripples by applying the GABA_A receptor antagonist gabazine to CA1 minislices of mouse hippocampus. Gabazine blocked spontaneously occurring sharp wave–ripple (SPW–R) activity. However, local application of KCl to the dendritic layer elicited excitatory sharp waves on which 200 Hz ripple oscillations were superimposed with equal temporal properties of native SPW–R. The activity also persisted in the additional presence of blockers of glutamatergic synaptic transmission. In contrast, synchrony was largely abolished after addition of gap junction blockers. Thus, GABAergic transmission appears to be involved in the generation of sharp waves but phasic inhibition is no prerequisite for the precise synchronization of hippocampal neurones during high-frequency oscillations at 200 Hz. Gap junctions on the other hand seem to be necessary to orchestrate coordinated activity within the ripple frequency domain.

(Received 19 November 2004; accepted after revision 14 January 2005; first published online 20 January 2005)
Corresponding author A. Draguhn: Institute of Physiology and Pathophysiology, Heidelberg University, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. Email: andreas.draguhn{at}urz.uni-heidelberg.de

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