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1 Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
Cell volume and cytosolic Ca2+ concentration ([Ca2+]i) were measured in rabbit macula densa (MD) cells loaded with calcein and Fura Red using confocal microscopy. [Ca2+]i was also analysed with Indo-1 and fura-2. We used isolated microperfused thick ascending limbs with attached glomerulus. The results showed that when the luminal NaCl concentration (‘NaCl’) was decreased from 35 to 10 mM, the cell volume decreased by 10.4%, and [Ca2+]i increased by 9.5%. This increase was inhibited in Ca2+-free solution. When luminal [NaCl] was changed from 35 to 135 mM, the cell volume increased by 15.1%, and [Ca2+]i did not change. The cell volume alterations were not different in Ca2+-free solutions. Using Indo-1, basal [Ca2+]i in MD cells was 107.8 nM. When luminal [NaCl] was changed from 135 to 10 mM, [Ca2]i increased by 23.5 nM. Using fura-2, the basal [Ca2+]i in MD cells was 115.3 nM, and when luminal [NaCl] was changed from 135 or 35 to 10 mM, [Ca2+]i change was 30.1 or 10.6 nM, respectively. An increase in [NaCl] caused no change in [Ca2+]i. In Ca2+-free solution, no change in [Ca2+]i occurred. A stepwise decrease in luminal [NaCl] resulted in a sigmoid increase in [Ca2+]i in MD cells. The steepest part of the curve was between 70 and 10 mM. In conclusion, we found that MD cells have cell volume regulation, and that [Ca2+]i elevation caused by decreased luminal [NaCl] is independent of the cell volume.
(Received 26 October 2004;
accepted after revision 14 January 2005;
first published online 20 January 2005)
Corresponding author A. E. G. Persson: Department of Medical Cell Biology, Uppsala University, BMC Box 571, S-75123 Uppsala, Sweden. Email: erik.persson{at}medcellbiol.uu.se
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