| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA
2 Department of Physiology, Semmelweis University, Budapest, H-1444, Hungary
3 Endocrinology and Reproduction Research Branch, NICHD, NIH, Bethesda, MD 20892, USA
Two-pore (2-P) domain potassium channels are implicated in the control of the resting membrane potential, hormonal secretion, and the amplitude, frequency and duration of the action potential. These channels are strongly regulated by hormones and neurotransmitters. Little is known, however, about the mechanism underlying their regulation. Here we show that phosphatidylinositol 4,5-bisphosphate (PIP2) gating underlies several aspects of 2-P channel regulation. Our results demonstrate that all four 2-P channels tested, TASK1, TASK3, TREK1 and TRAAK are activated by PIP2. We show that mechanical stimulation may promote PIP2 activation of TRAAK channels. For TREK1, TASK1 and TASK3 channels, PIP2 hydrolysis underlies inhibition by several agonists. The kinetics of inhibition by the PIP2 scavenger polylysine, and the inhibition by the phosphatidylinositol 4-kinase inhibitor wortmannin correlated with the level of agonist-induced inhibition. This finding suggests that the strength of channel PIP2 interactions determines the extent of PLC-induced inhibition. Finally, we show that PIP2 hydrolysis modulates voltage dependence of TREK1 channels and the unrelated voltage-dependent KCNQ1 channels. Our results suggest that PIP2 is a common gating molecule for K+ channel families despite their distinct structures and physiological properties.
(Received 21 December 2004;
accepted after revision 24 January 2005;
first published online 27 January 2005)
Corresponding authors D. E. Logothetis: Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA. Email: diomedes.logothetis{at}mssm.edu
P. Enyedi: Department of Physiology, Semmelweis University, Budapest, Hungary, H-1444. Email: enyedi{at}puskin.sote.hu
This article has been cited by other articles:
|
|
 |
|
  E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Sandoz, M. P. Tardy, S. Thummler, S. Feliciangeli, M. Lazdunski, and F. Lesage Mtap2 Is a Constituent of the Protein Network That Regulates Twik-Related K+ Channel Expression and Trafficking J. Neurosci., August 20, 2008; 28(34): 8545 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cohen, Y. Ben-Abu, S. Hen, and N. Zilberberg A Novel Mechanism for Human K2P2.1 Channel Gating: FACILITATION OF C-TYPE GATING BY PROTONATION OF EXTRACELLULAR HISTIDINE RESIDUES J. Biol. Chem., July 11, 2008; 283(28): 19448 - 19455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wang, U. L. Mirshahi, B. Liu, Z. Jia, T. Mirshahi, and H. Zhang Arachidonic Acid Activates Kir2.3 Channels by Enhancing Channel-Phosphatidyl-inositol 4,5-bisphosphate Interactions Mol. Pharmacol., April 1, 2008; 73(4): 1185 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Woodcock, X.-J. Du, M. E. Reichelt, and R. M. Graham Cardiac {alpha}1-adrenergic drive in pathological remodelling Cardiovasc Res, February 1, 2008; 77(3): 452 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Pochynyuk, Q. Tong, J. Medina, A. Vandewalle, A. Staruschenko, V. Bugaj, and J. D. Stockand Molecular Determinants of PI(4,5)P2 and PI(3,4,5)P3 Regulation of the Epithelial Na+ Channel J. Gen. Physiol., September 24, 2007; 130(4): 399 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Varas, C. N. Wyatt, and K. J. Buckler Modulation of TASK-like background potassium channels in rat arterial chemoreceptor cells by intracellular ATP and other nucleotides J. Physiol., September 1, 2007; 583(2): 521 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Liu, J. A. Enyeart, and J. J. Enyeart Angiotensin II inhibits native bTREK-1 K+ channels through a PLC-, kinase C-, and PIP2-independent pathway requiring ATP hydrolysis Am J Physiol Cell Physiol, August 1, 2007; 293(2): C682 - C695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Bryan Jr, B. K. Joseph, E. Lloyd, and N. J. Rusch Starring TREK-1: The Next Generation of Vascular K+ Channels Circ. Res., July 20, 2007; 101(2): 119 - 121. [Full Text] [PDF]
|
|
|
|
|
|
F. Charpentier Understanding the cardiac role of K2P channels: A new TASK for electrophysiologists Cardiovasc Res, July 1, 2007; 75(1): 5 - 6. [Full Text] [PDF]
|
|
|
|
|
|
C. Putzke, K. Wemhoner, F. B. Sachse, S. Rinne, G. Schlichthorl, X. T. Li, L. Jae, I. Eckhardt, E. Wischmeyer, H. Wulf, et al. The acid-sensitive potassium channel TASK-1 in rat cardiac muscle Cardiovasc Res, July 1, 2007; 75(1): 59 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Veale, L. E. Kennard, G. L. Sutton, G. MacKenzie, C. Sandu, and A. Mathie G{alpha}q-Mediated Regulation of TASK3 Two-Pore Domain Potassium Channels: The Role of Protein Kinase C Mol. Pharmacol., June 1, 2007; 71(6): 1666 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou, S. J. Martinez, M. Haber, E. V. Jones, D. Bouvier, G. Doucet, A. T. Corera, E. A. Fon, A. H. Zisch, and K. K. Murai EphA4 Signaling Regulates Phospholipase C{gamma}1 Activation, Cofilin Membrane Association, and Dendritic Spine Morphology J. Neurosci., May 9, 2007; 27(19): 5127 - 5138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pochynyuk, Q. Tong, A. Staruschenko, and J. D. Stockand Binding and direct activation of the epithelial Na+ channel (ENaC) by phosphatidylinositides J. Physiol., April 15, 2007; 580(2): 365 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mathie Neuronal two-pore-domain potassium channels and their regulation by G protein-coupled receptors J. Physiol., January 15, 2007; 578(2): 377 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Perez, F. J. White, and X.-T. Hu Dopamine D2 Receptor Modulation of K+ Channel Activity Regulates Excitability of Nucleus Accumbens Neurons at Different Membrane Potentials J Neurophysiol, November 1, 2006; 96(5): 2217 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, J. Han, and D. Kim Mechanism of inhibition of TREK-2 (K2P10.1) by the Gq-coupled M3 muscarinic receptor Am J Physiol Cell Physiol, October 1, 2006; 291(4): C649 - C656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Bryan Jr., J. You, S. C. Phillips, J. J. Andresen, E. E. Lloyd, P. A. Rogers, S. E. Dryer, and S. P. Marrelli Evidence for two-pore domain potassium channels in rat cerebral arteries Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H770 - H780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Z. Lan, P. Y. T. Wang, and C. E. Hill Modulation of hepatocellular swelling-activated K+ currents by phosphoinositide pathway-dependent protein kinase C Am J Physiol Cell Physiol, July 1, 2006; 291(1): C93 - C103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Pochynyuk, Q. Tong, A. Staruschenko, H.-P. Ma, and J. D. Stockand Regulation of the epithelial Na+ channel (ENaC) by phosphatidylinositides Am J Physiol Renal Physiol, May 1, 2006; 290(5): F949 - F957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Meuth, M. I. Aller, T. Munsch, T. Schuhmacher, T. Seidenbecher, P. Meuth, C. Kleinschnitz, H.-C. Pape, H. Wiendl, W. Wisden, et al. The Contribution of TWIK-Related Acid-Sensitive K+-Containing Channels to the Function of Dorsal Lateral Geniculate Thalamocortical Relay Neurons Mol. Pharmacol., April 1, 2006; 69(4): 1468 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, E. M. Talley, N. Patel, A. Gomis, W. E. McIntire, B. Dong, F. Viana, J. C. Garrison, and D. A. Bayliss Inhibition of a background potassium channel by Gq protein {alpha}-subunits PNAS, February 28, 2006; 103(9): 3422 - 3427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pochynyuk, A. Staruschenko, Q. Tong, J. Medina, and J. D. Stockand Identification of a Functional Phosphatidylinositol 3,4,5-Trisphosphate Binding Site in the Epithelial Na+ Channel J. Biol. Chem., November 11, 2005; 280(45): 37565 - 37571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Enkvetchakul, I. Jeliazkova, and C. G. Nichols Direct Modulation of Kir Channel Gating by Membrane Phosphatidylinositol 4,5-Bisphosphate J. Biol. Chem., October 28, 2005; 280(43): 35785 - 35788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Murbartian, Q. Lei, J. J. Sando, and D. A. Bayliss Sequential Phosphorylation Mediates Receptor- and Kinase-induced Inhibition of TREK-1 Background Potassium Channels J. Biol. Chem., August 26, 2005; 280(34): 30175 - 30184. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
