| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institute for Biomedical Sciences, School of Medical Sciences, University of Sydney F13, NSW 2006, Australia
The mechanisms of muscle fatigue were studied in small muscle bundles and single fibres isolated from the flexor digitorum brevis of the mouse. Fatigue caused by repeated isometric tetani was accelerated at body temperature (37°C) when compared to room temperature (22°C). The membrane-permeant reactive oxygen species (ROS) scavenger, Tiron (5 mM), had no effect on the rate of fatigue at 22°C but slowed the rate of fatigue at 37°C to that observed at 22°C. Single fibres were microinjected with indo-1 to measure intracellular calcium. In the accelerated fatigue at 37°C the tetanic [Ca2+]i did not change significantly and the decline of maximum Ca2+-activated force was similar to that observed at 22°C. The cause of the greater rate of fatigue at 37°C was a large fall in myofibrillar Ca2+ sensitivity. In the presence of Tiron, the large fall in Ca2+ sensitivity was abolished and the usual decline in tetanic [Ca2+]i was observed. This study confirms the importance of ROS in fatigue at 37°C and shows that the mechanism of action of ROS is a decline in myofibrillar Ca2+ sensitivity.
(Received 19 January 2005;
accepted after revision 16 February 2005;
first published online 17 February 2005)
Corresponding author D. G. Allen: Institute for Biomedical Sciences, School of Medical Sciences, University of Sydney F13, NSW 2006, Australia. Email: davida{at}physiol.usyd.edu.au
This article has been cited by other articles:
|
|
 |
|
  A. Dutta, K. Ray, V. K. Singh, P. Vats, S. N. Singh, and S. B. Singh L-carnitine supplementation attenuates intermittent hypoxia-induced oxidative stress and delays muscle fatigue in rats Exp Physiol, October 1, 2008; 93(10): 1139 - 1146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Murphy, T. L. Dutka, and G. D. Lamb Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres J. Physiol., April 15, 2008; 586(8): 2203 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Ferreira and M. B. Reid Muscle-derived ROS and thiol regulation in muscle fatigue J Appl Physiol, March 1, 2008; 104(3): 853 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Fitts The cross-bridge cycle and skeletal muscle fatigue J Appl Physiol, February 1, 2008; 104(2): 551 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Allen, G. D. Lamb, and H. Westerblad Impaired calcium release during fatigue J Appl Physiol, January 1, 2008; 104(1): 296 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Bruton, N. Place, T. Yamada, J. P. Silva, F. H. Andrade, A. J. Dahlstedt, S.-J. Zhang, A. Katz, N.-G. Larsson, and H. Westerblad Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice J. Physiol., January 1, 2008; 586(1): 175 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Edwards, W. A. Macdonald, C. van der Poel, and D. G. Stephenson O2bullet production at 37{degrees}C plays a critical role in depressing tetanic force of isolated rat and mouse skeletal muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C650 - C660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. van der Poel and D. G. Stephenson Effects of elevated physiological temperatures on sarcoplasmic reticulum function in mechanically skinned muscle fibers of the rat Am J Physiol Cell Physiol, July 1, 2007; 293(1): C133 - C141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, T. Mishima, M. Sakamoto, M. Sugiyama, S. Matsunaga, and M. Wada Myofibrillar protein oxidation and contractile dysfunction in hyperthyroid rat diaphragm J Appl Physiol, May 1, 2007; 102(5): 1850 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. van der Poel, J. N. Edwards, W. A. Macdonald, and D. G. Stephenson Mitochondrial superoxide production in skeletal muscle fibers of the rat and decreased fiber excitability Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1353 - C1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sahlin, J. S. Nielsen, M. Mogensen, and M. Tonkonogi Repeated static contractions increase mitochondrial vulnerability toward oxidative stress in human skeletal muscle J Appl Physiol, September 1, 2006; 101(3): 833 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, T. Mishima, M. Sakamoto, M. Sugiyama, S. Matsunaga, and M. Wada Oxidation of myosin heavy chain and reduction in force production in hyperthyroid rat soleus J Appl Physiol, May 1, 2006; 100(5): 1520 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Moopanar and D. G. Allen The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitol J. Physiol., February 15, 2006; 571(1): 191 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G Allen, N. P Whitehead, and E. W Yeung Mechanisms of stretch-induced muscle damage in normal and dystrophic muscle: role of ionic changes J. Physiol., September 15, 2005; 567(3): 723 - 735. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
