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J Physiol Volume 564, Number 3, 751-763, May 1, 2005 DOI: 10.1113/jphysiol.2005.084517
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Early expression of AMPA receptors and lack of NMDA receptors in developing rat climbing fibre synapses

Philippe Lachamp1, Bénedicte Balland1, Fabien Tell1, Agnès Baude1, Caroline Strube1, Marcel Crest1 and Jean-Pierre Kessler1

1 Laboratoire de Neurophysiologie cellulaire, UMR CNRS 6150, Centre National de la Recherche Scientifique et Université de la Mediterrannée, IFR Jean Roche, Faculté de Médecine, Bd Pierre Dramard, F13916 Marseille cedex 20, France

Whether nascent glutamatergic synapses acquire their AMPA receptors constitutively or via a regulated pathway triggered by pre-existing NMDA receptor activation is still an open issue. Here, we provide evidence that some glutamatergic synapses develop without expressing NMDA receptors. Using immunocytochemistry, we showed that synapses between developing rat climbing fibres and Purkinje cells expressed GluR2-containing AMPA receptors as soon as they were formed (i.e. on embryonic day 19) but never carried detectable NMDA receptors. This was confirmed by electrophysiological recordings. Excitatory synaptic currents were recorded in Purkinje cells as early as P0. However, no NMDA receptor-mediated component was found in either spontaneous or evoked synaptic responses. In addition, we ruled out a possible role of extrasynaptic NMDA receptors by showing that AMPA receptor clustering at nascent climbing fibre synapses was not modified by chronic in utero NMDA receptor blockade.

(Received 7 February 2005; accepted after revision 23 February 2005; first published online 24 February 2005)
Corresponding author J.-P. Kessler: LNPC, UMR CNRS 6150, Faculté de Médecine Nord, Bd Pierre Dramard, F13916 Marseille cedex 20, France. Email: kessler.jp{at}jean-roche.univ-mrs.fr




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