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Molecular And Genomic |
1 Department of Molecular and Cellular Physiology
2 Departments of Genetics, Medicine, and Molecular Biophysics & Biochemistry, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA
3 Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
The serine–threonine kinase WNK3 modulates Cl– transport into and out of cells through its regulation of SLC12A cation–Cl– cotransporters, implicating it as (one of) the long-sought Cl–/volume-sensitive kinase(s). Integrators in homeostatic systems regulate structurally diverse but functionally coupled elements. For example, the related kinase WNK4 regulates the Na+–Cl– cotransporter (NCC), paracellular Cl– flux, and the K+ channel ROMK1 (Kir1.1) to maintain renal NaCl and K+ homeostasis; mutations in PRKWNK4, encoding WNK4, cause a Mendelian disease featuring hypertension and hyperkalaemia. It is known that WNK3 is expressed in the nephron's distal convoluted tubule (DCT) and stimulates NCC activity. Here, we show that WNK3 is also expressed in cortical and outer medullary collecting duct principal cells. Accordingly, we tested WNK3's effect on the mediators of NaCl and K+ handling in these nephron segments – the epithelial sodium channel (ENaC), paracellular Cl– flux, and ROMK1 – using established model systems. WNK3 did not alter paracellular Cl– flux in tetracycline-responsive MDCK II cells, nor affect amiloride-sensitive currents when coexpressed with ENaC in Xenopus laevis oocytes. However, additional coexpression studies in oocytes revealed WNK3 inhibited the renal-specific K+ channel ROMK1 activity greater than 5.5-fold (P < 0.0001) by altering its plasmalemmal surface expression; WNK3 did not affect ROMK1's conductance or open/closed probability. In contrast, WNK3 had no effect on the activity of the cardiac long-QT syndrome K+ channel KCNQ1/KCNE1 when coexpressed in oocytes. Inhibition of ROMK1 is independent of WNK3's catalytic activity and is mediated by WNK3's carboxyl terminus – a mechanism distinct from its known kinase-dependent activation of NCC. A kinase-inactivating point mutation or a missense mutation homologous to one in WNK4 that causes disease produced a gain-of-function effect, enhancing WNK3's inhibition of ROMK1 greater than 2.5-fold relative to wild-type kinase (P < 0.0001). The magnitude and specificity of WNK3's effects at both NCC and ROMK1, its coexpression with its targets in the distal nephron, and the established in vivo effect of WNK4 at these same targets provide evidence that WNK3's action is physiologically relevant. WNK3 is probably a component of one of the mechanisms that determines the balance between renal NaCl reabsorption and K+ secretion.
(Received 22 November 2005;
accepted after revision 14 December 2005;
first published online 15 December 2005)
Corresponding author S. Hebert: Department of Molecular and Cellular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA. Email: steven.hebert{at}yale.edu
This article has been cited by other articles:
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  M. R. Dorwart, N. Shcheynikov, Y. Wang, S. Stippec, and S. Muallem SLC26A9 is a Cl channel regulated by the WNK kinases J. Physiol., October 1, 2007; 584(1): 333 - 345. [Abstract] [Full Text] [PDF]
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J. L. Pluznick and S. C. Sansom BK channels in the kidney: role in K+ secretion and localization of molecular components Am J Physiol Renal Physiol, September 1, 2006; 291(3): F517 - F529. [Abstract] [Full Text] [PDF]
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