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This Article Full Text Full Text (PDF) All Versions of this Article: 575/3/777    most recent jphysiol.2006.112409v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paterson, J. M. Articles by Dutia, M. B. Search for Related Content PubMed PubMed Citation Articles by Paterson, J. M. Articles by Dutia, M. B. Related Collections Neuroscience

¹ Centre for Integrative Physiology, Biomedical Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK
² Astellas CNS Research in Edinburgh, The Chancellor's Building, New Royal Infirmary, Little France Crescent, Edinburgh EH16 4SB, UK
³ The Queen's Medical Research Institute, New Royal Infirmary, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

The molecular mechanisms of neural and synaptic plasticity in the vestibular nuclei during ‘vestibular compensation’, the behavioural recovery that follows deafferentation of one inner ear, are largely unknown. In this study we have used differential proteomics techniques to determine changes in protein expression in ipsi-lesional and contra-lesional medial vestibular nuclei (MVN) of rats, 1 week after either sham surgery or unilateral labyrinthectomy (UL). A systematic comparison of 634 protein spots in two-dimensional electrophoresis gels across five experimental conditions revealed 54 spots, containing 26 proteins whose level was significantly altered 1 week post-UL. The axon-guidance-associated proteins neuropilin-2 and dehydropyriminidase-related protein-2 were upregulated in the MVN after UL. Changes in levels of further specific proteins indicate a coordinated upregulation of mitochondrial function, ATP biosynthesis and phosphate metabolism in the vestibular nuclei 1 week post-UL. These may reflect the metabolic energy demands of processes such as gliosis, neuronal outgrowth and synaptic remodelling that occur after UL. Our findings suggest novel roles for axon elaboration and guidance molecules, as well as mitochondrial and metabolic regulatory proteins, in the post-lesional physiology of the MVN during vestibular system plasticity.

(Received 27 April 2006; accepted after revision 4 July 2006; first published online 6 July 2006)
Corresponding author M. B. Dutia: Centre for Integrative Physiology, Biomedical Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK. Email: m.b.dutia{at}ed.ac.uk

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