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This Article Full Text Full Text (PDF) Supplemental data All Versions of this Article: 580/2/463    most recent jphysiol.2006.125005v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Canepari, M. Articles by Zecevic, D. Search for Related Content PubMed PubMed Citation Articles by Canepari, M. Articles by Zecevic, D. Related Collections Neuroscience

¹ Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
² National Institute for Medical Research, London, UK
³ Marine Biological Laboratory, Woods Hole, MA 02543, USA

The non-linear and spatially inhomogeneous interactions of dendritic membrane potential signals that represent the first step in the induction of activity-dependent long-term synaptic plasticity are not fully understood, particularly in dendritic regions which are beyond the reach of electrode measurements. We combined voltage-sensitive-dye recordings and Ca²⁺ imaging of hippocampal CA1 pyramidal neurons to study large regions of the dendritic arbor, including branches of small diameter (distal apical and oblique dendrites). Dendritic membrane potential transients were monitored at high spatial resolution and correlated with supra-linear [Ca²⁺]_i changes during one cycle of a repetitive patterned stimulation protocol that typically results in the induction of long-term potentiation (LTP). While the increase in the peak membrane depolarization during coincident pre- and post-synaptic activity was required for the induction of supra-linear [Ca²⁺]_i signals shown to be necessary for LTP, the change in the baseline-to-peak amplitude of the backpropagating dendritic action potential (bAP) was not critical in this process. At different dendritic locations, the baseline-to-peak amplitude of the bAP could be either increased, decreased or unaltered at sites where EPSP–AP pairing evoked supra-linear summation of [Ca²⁺]_i transients. We suggest that modulations in the bAP baseline-to-peak amplitude by local EPSPs act as a mechanism that brings the membrane potential into the optimal range for Ca²⁺ influx through NMDA receptors (0 to –15 mV); this may require either boosting or the reduction of the bAP, depending on the initial size of both signals.

(Received 16 November 2006; accepted after revision 30 January 2007; first published online 1 February 2007)
Corresponding author D. Zecevic: Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.  Email: dejan.zecevic{at}yale.edu

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