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¹ Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, UK

An essential step in understanding fast synaptic transmission is to establish the activation mechanism of synaptic receptors. The purpose of this work was to extend our detailed single-channel kinetic characterization of 1 glycine channels from rat recombinant receptors to native channels from juvenile (postnatal day 12–16) rat spinal cord slices. In cell-attached patches from ventral horn neurones, 1 mM glycine elicited clusters of channel openings to a single conductance level (41 ± 1 pS, n = 12). This is similar to that of recombinant heteromers. However, fewer than 1 in 100 cell-attached patches from spinal neurones contained glycine channels. Outside-out patches gave a much higher success rate, but glycine channels recorded in this configuration appeared different, in that clusters opened to three conductance levels (28 ± 2, 38 ± 1 and 46 ± 1 pS, n = 7, one level per cluster, all levels being detected in each patch). Furthermore, open period properties were different for the different conductances. As a consequence of this, the only recordings suitable for kinetic analysis were the cell-attached ones. Low channel density precluded recording at glycine concentrations other than 1 mM, but the 1 mM data allowed us to estimate the fully bound gating constants by global model fitting of the ‘flip’ mechanism of Burzomato and co-workers. Our results suggest that glycine receptors on ventral horn neurones in the juvenile rat are heteromers and have fast gating, similar to that of recombinant 1 receptors.

(Received 28 November 2006; accepted after revision 31 January 2007; first published online 1 February 2007)
Corresponding author M. Beato: Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, UK.  Email: m.beato{at}ucl.ac.uk

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