HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Free via Open Access: OA OA Full Text Full Text (PDF) OA All Versions of this Article: 582/3/1141    most recent jphysiol.2007.133488v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mentis, G. Z. Articles by Navarrete, R. Search for Related Content PubMed PubMed Citation Articles by Mentis, G. Z. Articles by Navarrete, R. Related Collections Neuroscience

¹Division of Neuroscience and Mental Health, Department of Cellular & Molecular Neuroscience, Imperial College London, Fulham Palace Road, London W6 8RF, UK ²Section of Developmental Neurobiology, Building 35, Room 3C1010, NINDS, NIH, Bethesda, MD 20892, USA

Early in development, motoneurones are critically dependent on their target muscles for survival and differentiation. Previous studies have shown that neonatal axotomy causes massive motoneurone death and abnormal function in the surviving motoneurones. We have investigated the electrophysiological and morphological properties of motoneurones innervating the flexor tibialis anterior (TA) muscle during the first week after a neonatal axotomy, at a time when the motoneurones would be either in the process of degeneration or attempting to reinnervate their target muscles. We found that a large number (75%) of TA motoneurones died within 3 weeks after neonatal axotomy. Intracellular recordings revealed a marked increase in motoneurone excitability, as indicated by changes in passive and active membrane electrical properties. These changes were associated with a shift in the motoneurone firing pattern from a predominantly phasic pattern to a tonic pattern. Morphologically, the dendritic tree of the physiologically characterized axotomized cells was significantly reduced compared with age-matched normal motoneurones. These data demonstrate that motoneurone electrical properties are profoundly altered shortly after neonatal axotomy. In a subpopulation of the axotomized cells, abnormally high motoneurone excitability (input resistance significantly higher compared with control cells) was associated with a severe truncation of the dendritic arbor, suggesting that this excitability may represent an early electrophysiological correlate of motoneurone degeneration.

(Received 27 March 2007; accepted after revision 17 May 2007; first published online 17 May 2007)
Corresponding author G. Z. Mentis: Laboratory of Neural Control, Building 35, Room 3C1010, NINDS, NIH, Bethesda, MD 20892, USA. Email: mentisg{at}ninds.nih.gov