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SKELETAL MUSCLE AND EXERCISE |
expression is influenced by muscle activity and induces slow muscle properties in adult rat muscles after somatic gene transfer
1 Department of Molecular Biosciences, University of Oslo, Oslo, Norway
2 National Institute of Health, Section of Molecular Neurobiology, NICHD, Bethesda, MD, USA
The effects of exercise on skeletal muscle are mediated by a coupling between muscle electrical activity and gene expression. Several activity correlates, such as intracellular Ca2+, hypoxia and metabolites like free fatty acids (FFAs), might initiate signalling pathways regulating fibre-type-specific genes. FFAs can be sensed by lipid-dependent transcription factors of the peroxisome proliferator-activated receptor (PPAR) family. We found that the mRNA for the predominant muscle isoform, PPAR
, was three-fold higher in the slow/oxidative soleus compared to the fast/glycolytic extensor digitorum longus (EDL) muscle. In histological sections of the soleus, the most oxidative fibres display the highest levels of PPAR protein. When the soleus muscle was stimulated electrically by a pattern mimicking fast/glycolytic IIb motor units, the mRNA level of PPAR was reduced to less than half within 24 h. In the EDL, a three-fold increase was observed after slow type I-like electrical stimulation. When a constitutively active form of PPAR was overexpressed for 14 days in normally active adult fibres after somatic gene transfer, the number of I/IIa hybrids in the EDL more than tripled, IIa fibres increased from 14% to 25%, and IIb fibres decreased from 55% to 45%. The level of succinate dehydrogenase activity increased and size decreased, also when compared to normal fibres of the same type. Thus PPAR can change myosin heavy chain, oxidative enzymes and size locally in muscle cells in the absence of general exercise. Previous studies on PPAR in muscle have been performed in transgenic animals where the transgene has been present during muscle development. Our data suggest that PPAR can mediate activity effects acutely in pre-existing adult fibres, and thus is an important link in excitation–transcription coupling.
(Received 21 March 2007;
accepted after revision 20 April 2007;
first published online 26 April 2007)
Corresponding author K. Gundersen: Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindern, N-0316 Oslo, Norway. Email: kgunder{at}imbv.uio.no
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