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¹ Department of Physiology Anatomy and Genetics, Sherrington Building, Parks Road, Oxford OX1 3PT, UK

The carotid body's physiological role is to sense arterial oxygen, CO₂ and pH. It is however, also powerfully excited by inhibitors of oxidative phosphorylation. This latter observation is the cornerstone of the mitochondrial hypothesis which proposes that oxygen is sensed through changes in energy metabolism. All of these stimuli act in a similar manner, i.e. by inhibiting a background TASK-like potassium channel (K_B) they induce membrane depolarization and thus neurosecretion. In this study we have evaluated the role of ATP in modulating K_B channels. We find that K_B channels are strongly activated by MgATP (but not ATP₄^–) within the physiological range (K_1/2 = 2.3 mM). This effect was mimicked by other Mg-nucleotides including GTP, UTP, AMP-PCP and ATP--S, but not by PP_i or AMP, suggesting that channel activity is regulated by a Mg-nucleotide sensor. Channel activation by MgATP was not antagonized by either 1 mM AMP or 500 µM ADP. Thus MgATP is probably the principal nucleotide regulating channel activity in the intact cell. We therefore investigated the effects of metabolic inhibition upon both [Mg²⁺]_i, as an index of MgATP depletion, and channel activity in cell-attached patches. The extent of increase in [Mg²⁺]_i (and thus MgATP depletion) in response to inhibition of oxidative phosphorylation were consistent with a decline in [MgATP]_i playing a prominent role in mediating inhibition of K_B channel activity, and the response of arterial chemoreceptors to metabolic compromise.

(Received 1 May 2007; accepted after revision 3 July 2007; first published online 5 July 2007)
Corresponding author K. J. Buckler: Department of Physiology Anatomy and Genetics, Sherrington Building, Parks Road, Oxford OX1 3PT, UK. Email: keith.buckler{at}physiol.ox.ac.uk

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