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This Article Full Text Full Text (PDF) All Versions of this Article: 584/3/1011    most recent jphysiol.2007.140673v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nilsson, E. Articles by Vaag, A. Search for Related Content PubMed PubMed Citation Articles by Nilsson, E. Articles by Vaag, A. Related Collections Skeletal Muscle and Exercise

¹ Steno Diabetes Center, Gentofte, Denmark
² Department of Clinical Sciences, Diabetes and Endocrinology
³ Department of Clinical Sciences, Clinical Obesity, Malmö University Hospital, Lund University, Sweden

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors regulating the expression of genes involved in lipid and glucose metabolism in a complex and to some extent unknown manner. Our aim was to study the impact of different factors on PPAR mRNA expression in human skeletal muscle on one side, and the impact of PPAR mRNA expression on these factors, including glucose and lipid metabolism, aerobic capacity, fibre type composition and lipid profile, on the other side. PPAR mRNA levels were quantified by real-time PCR in muscle biopsies from 176 young and elderly monozygotic and dizygotic twins. Young twins had significantly increased PPAR mRNA levels compared with elderly twins. A 2 h hyperinsulinaemic euglycaemic clamp had no significant effect on PPAR mRNA levels. Biometric models were calculated for basal PPAR mRNA expression to estimate the degree of genetic versus environmental influence. In both young and elderly twins there was a substantial genetic component influencing basal PPAR mRNA levels. In a regression model, the muscle PPAR mRNA expression was correlated to birth weight, central adiposity and age. The level of PPAR mRNA was also positively correlated with markers for oxidative muscle fibres. However, in this apparently healthy study population, we found no correlations between PPAR mRNA expression and aerobic capacity, lipid profile or glucose and lipid metabolism. In conclusion, we provide evidence that mRNA expression of PPAR in human skeletal muscle is under genetic control but also influenced by factors such as age, birth weight and central adiposity.

(Received 13 July 2007; accepted after revision 11 September 2007; first published online 13 September 2007)
Corresponding author E. Nilsson: Steno Diabetes Center, Niels Steensens Vej 1, DK-2820 Gentofte, Denmark. Email: emmn{at}steno.dk