J Physiol Wellcome Trust-funded researchers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 586, Number 12, 3005-3016, June 15, 2008 DOI: 10.1113/jphysiol.2007.147348
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
586/12/3005    most recent
jphysiol.2007.147348v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Hansen, M.
Right arrow Articles by Langberg, H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hansen, M.
Right arrow Articles by Langberg, H.
Related Collections
Right arrow Integrative

INTEGRATIVE

Ethinyl oestradiol administration in women suppresses synthesis of collagen in tendon in response to exercise

 Mette Hansen1, Satu O. Koskinen1, Susanne G. Petersen1, Simon Doessing1, Jan Frystyk2, Allan Flyvbjerg2, Eva Westh1, S. Peter Magnusson1, Michael Kjaer1 and Henning Langberg1

1 Institute of Sports Medicine, Bispebjerg Hospital, DK-2400 NV, and Faculty of Health Sciences, University of Copenhagen, Denmark
2 Medical Research Laboratories, Clinical Institute and Medical Department M (Diabetes and Endocrinology), Aarhus University Hospital, DK-8000, Aarhus C, Denmark

Women are at greater risk than men of sustaining certain kinds of injury and diseases of collagen-rich tissues. To determine whether a high level of oestradiol has an acute influence on collagen synthesis in tendons at rest and in response to exercise, one-legged kicking exercise was performed for 60 min at 67% of maximum power by healthy, young oral contraceptive (OC) users when circulating synthetic (ethinyl) oestradiol was high (n = 11, HE-OC) and compared to similar women who had never used OCs when circulating endogenous oestrogen was low (n = 12, LE-NOC). Interstitial fluid was collected 24 h post-exercise through microdialysis catheters placed anterior to the patellar tendon in both legs and subsequently analysed for the amino-terminal propeptide of type I collagen (PINP), a marker of tendon collagen synthesis. To determine the long-term effect of OC usage, patellar tendon cross-sectional area (CSA) was measured by magnetic resonance imaging (MRI). A lower exercise-induced increase in tendon collagen synthesis was observed in HE-OC than in LE-NOC ({Delta}PINP (mean ± S.E.M.) 1.5 ± 5.3 versus 24.2 ± 9.4 ng ml–1, P < 0.05). Furthermore, serum and the interstitial peritendinous tissue concentrations of insulin-like growth factor I (IGF-I) and IGF-binding proteins showed a reduced bioavailability in HE-OC compared with results in LE-NOC. No difference in patellar tendon CSA was observed between groups. In conclusion, the selective increase in tendon collagen synthesis in LE-NOC but not HE-OC 24 h post-exercise is consistent with the hypothesis that oestradiol inhibits exercise-induced collagen synthesis in human tendon. The mechanism behind this is either a direct effect of oestradiol, or an indirect effect via a reduction in levels of free IGF-I. However, the data did not indicate any long-term effect on tendon size associated with chronic OC use.

(Received 26 October 2007; accepted after revision 16 April 2008; first published online 17 April 2008)
Corresponding author M. Hansen: Institute of Sports Medicine, Copenhagen, Build. 8, Bispebjerg Hospital, Bispebjerg Bakke 23, DK-2400 Copenhagen NV, Denmark. Email: mh19{at}bbh.regionh.dk






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 The Physiological Society.