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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 586/16/3783    most recent jphysiol.2008.155879v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Google Scholar Articles by Platel, J.-C. Articles by Bordey, A. PubMed PubMed Citation Articles by Platel, J.-C. Articles by Bordey, A. Related Collections Neuroscience

¹ Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University, New Haven, CT 06520-8082, USA

In the postnatal subventricular zone (SVZ), neuroblasts migrate in chains along the lateral ventricle towards the olfactory bulb. AMPA/kainate receptors as well as metabotropic glutamate receptors subtype 5 (mGluR5) are expressed in SVZ cells. However, the cells expressing these receptors and the function of these receptors remain unexplored. We thus examined whether SVZ neuroblasts express mGluR5 and Ca²⁺-permeable kainate receptors in mouse slices. Doublecortin (DCX)-immunopositive cells (i.e. neuroblasts) immunostained positive for mGluR5 and GLU_K5-7-containing kainate receptors. RT-PCR from 10 GFP-fluorescent cell aspirates obtained in acute slices from transgenic mice expressing green fluorescent protein (GFP) under the DCX promoter showed mGluR5 and GLU_K5 receptor mRNA in SVZ neuroblasts. Patch-clamp data suggest that 60% of neuroblasts express functional GLU_K5-containing receptors. Activation of mGluR5 and GLU_K5-containing receptors induced Ca²⁺ increases in 50% and 60% of SVZ neuroblasts, respectively, while most neuroblasts displayed GABA_A-mediated Ca²⁺ responses. To examine the effects of these receptors on the speed of neuroblast migration, we developed a whole-mount preparation of the entire lateral ventricle from postnatal day (P) 20–25 DCX-GFP mice. The GABA_A receptor (GABA_AR) antagonist bicuculline increased the speed of neuroblast migration by 27%, as previously reported in acute slices. While the mGluR5 antagonist MPEP did not affect the speed of neuroblast migration, the homomeric and heteromeric GLU_K5 receptor antagonists, NS3763 and UB302, respectively, increased the migration speed by 38%. These data show that although both GLU_K5 receptor and mGluR5 activations increase Ca²⁺ in neuroblasts, only GLU_K5 receptors tonically reduce the speed of neuroblast migration along the lateral ventricle.

(Received 25 April 2008; accepted after revision 18 June 2008; first published online 19 June 2008)
Corresponding author A. Bordey: Department of Neurosurgery, Yale University School of Medicine, 333 Cedar Street, FMB 422, New Haven, CT 06520-8082, USA.  Email: angelique.bordey{at}yale.edu

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