alpha-Adrenergic effects on Na+-K+ pump current in guinea-pig ventricular myocytes

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$alpha$ -Adrenergic effects on Na⁺-K⁺ pump current in guinea-pig ventricular myocytes

Y. Wang, J. Gao, R. T. Mathias, I. S. Cohen, X. Sun and G. J. Baldo

Department of Physiology & Biophysics, State University of New York at Stony Brook, NY 11794-8661, USA

Received 23 October 1997; accepted after revision 9 February 1998.

ABSTRACT

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Abstract
Introduction
Methods
Results
Discussion
References

The whole-cell patch clamp was employed to study Na⁺-K⁺ pump current (I_p) in acutely isolated myocytes. $alpha$ -Adrenergic receptors were activated with noradrenaline (NA) after blocking $beta$ -adrenergic receptors with propranolol. I_p was measured as the current blocked by strophanthidin (Str).

Activation of $alpha$ -receptors by NA increased I_p in a concentration-dependent manner. The K_0�5 depended on intracellular calcium ([Ca²⁺]_i), however maximal stimulation did not. At 15 nM [Ca²⁺]_i the K_0�5 was 219 nM NA whereas at 1�4 µm [Ca²⁺]_i it was 3 nM.

The voltage dependence of I_p was not shifted by NA at either high or low [Ca²⁺]_i. At each voltage, maximal stimulation of I_p was 14-15 %.

Staurosporine (St), an inhibitor of protein kinase C (PKC), eliminated the $alpha$ -receptor-mediated stimulation of I_p at either high or low[Ca²⁺]_i.

The stimulation of I_p was independent of changes in intracellular sodium or external potassium concentrations, and did not reflect a change in affinity for Str.

Phenylephrine, methoxamine and metaraminol, three selective $alpha$ ₁-adrenergic agonists, stimulate I_p in a similar manner to NA. Stimulation of I_p by NA was eliminated by prazosin, an $alpha$ ₁-antagonist, but was unaffected by yohimbine, an $alpha$ ₂-antagonist.

We conclude noradrenaline activates ventricular $alpha$ ₁-receptors, which are specifically coupled via PKC to increase Na⁺-K⁺ pump current. The sensitivity of the coupling is [Ca²⁺]_idependent, however the maximal increase in pump current is [Ca²⁺]_i and voltage independent.

INTRODUCTION

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Abstract
Introduction
Methods
Results
Discussion
References

The Na⁺-K⁺ pump is an electrogenic ion transporter in the plasma membrane of all mammalian cells. Its primary role is to maintain low intracellular sodium and high intracellular potassium; however, in the heart it has other important functions. It hyperpolarizes the cardiac cell by producing a net outward current, I_p, that has a significant effect on the duration of the plateau phase of the cardiac action potential (Gadsby & Cranefield, 1980). Modulation of I_p therefore affects myocardial functions like rhythm, conduction, and force of contraction.

The sympathetic nervous system is a major regulator of the Na⁺-K⁺ pump in cardiac muscle, as previously reported by Falk & Cohen (1984) and Desilets & Baumgarten (1988), and in work from our laboratory. For example, $beta$ -receptor activation by isoprenaline (Iso) at low [Ca²⁺]_i inhibited I_p whereas at high [Ca²⁺]_i inhibition was eliminated but Iso shifted the I-V relationship of I_p in the negative direction on the voltage axis (Gao, Mathias, Cohen, Shi & Baldo, 1996). Both effects were mediated by a phosphorylation step via the cAMP-dependent PKA pathway (Gao, Mathias, Cohen & Baldo, 1992; Gao, Cohen, Mathias & Baldo, 1994). Acetylcholine (ACh) has no effect on I_p in the absence of Iso, but reverses the $beta$ -effects (Gao, Mathias, Cohen & Baldo, 1997a).

During the last decade, there has been growing evidence for modulation of I_p by $alpha$ -adrenergic receptors. Studies in canine Purkinje myocytes and fibres (Shah, Cohen, Rosen, 1988; Zaza, Kline & Rosen, 1990), rat papillary muscle (Ertl, Jahnel, Nawrath, Carmeliet & Vereecke, 1991), rat atrial muscle (Terzic, Anagnostopoulos & Vogel, 1991), beating guinea-pig ventricular muscle (Wilde & Kleber, 1991), and rat ventricular myocytes (Williamson, Kennedy, Seifen, Lindemann & Stimers, 1993) suggest $alpha$ -activation stimulates I_p. But all these studies used indirect measurements, such as the ouabain-sensitive hyperpolarization, change in holding current or change in [Na⁺]_i induced by $alpha$ -activation. Moreover, Tohse, Hattori, Nakaya & Kanno (1987a) reported that stimulation of the Na⁺-K⁺ pump was not involved in the $alpha$ -mediated hyperpolarization in quiescent rat papillary muscle. Thus the data are indirect and in some cases contradictory. The purpose of this study is to measure directly changes in pump current resulting from $alpha$ -activation, to determine whether these changes are specifically or indirectly coupled to $alpha$ -receptors and to determine which $alpha$ -receptor subtype is involved and which intracellular cascade mediates the effect.

METHODS

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Abstract
Introduction
Methods
Results
Discussion
References

Studies were performed on single ventricular myocytes that were enzymatically isolated from guinea-pig hearts (Gao et al. 1992). Male guinea-pigs, weighing 300-500 g, were killed with sodium pentobarbitone solution (1 ml of 360 mg ml^-1) by peritoneal injection. The isolated cells were stored for use the following day at 4�C in Kraft-Brühe (KB) solution containing (mM): K₂HPO₄, 30; KCl, 83; MgSO₄, 5; sodium pyruvic acid, 5; $beta$ -hydroxybutyric acid, 5; creatine, 5; taurine, 20; glucose, 10; Hepes, 5; EGTA, 0�5; KOH, 2; Na₂-ATP, 5; pH 7�2.

Cells were placed in a 0�65 ml lucite bath, which could be superfused at room temperature (25-27�C) with a solution flow of 3 ml min^-1. Solutions were changed with a manual valve leading to the bath; the dead space was 0�15 ml. An Axopatch-1B amplifier (Axon Instruments) and the whole-cell patch-clamp technique were employed to observe cell membrane current. Patch pipette resistances were 1-3 M $Omega$ prior to sealing. Myocytes were clamped to 0 mV and I_p was defined as the inward shift in holding current observed during bath application of 0�5 mM strophanthidin (Str), a selective and reversible inhibitor of the Na⁺-K⁺ pump. All patch-clamp data were displayed on a digital storage oscilloscope and recorded on computer disk for later analysis. The sampling rate was 500 ms point^-1 and the data were low-pass filtered at 10 Hz during steady-state recording. For each experiment both control and test pump currents were obtained in the same cell and the ratio of the test I_p to control I_p was calculated. All values are given as means � standard deviation.

The patch electrode solution contained (mM): sodium aspartic acid, 50; potassium aspartic acid, 20; CsOH, 30 ; TEACl, 20; MgSO₄, 5; Hepes, 5; EGTA, 11; glucose, 10; Na₂-ATP, 5; GTP, 0�2; CaCl₂, 1 (giving a free [Ca²⁺]_i of 15 nM ), and pH was adjusted to 7�2 using $beta$ CsOH. The superfusion solutions include control and test solution. The former was Tyrode solution containing (mM): NaCl, 137�7; KCl, 5�4; NaOH, 2�3; MgCl₂, 1; glucose, 10; Hepes, 5; BaCl₂, 2; CdCl₂, 1; propranolol, 0�1; pH 7�4. The latter was prepared by adding $alpha$ -agonist, $alpha$ -agonist plus $alpha$ -antagonist and/or the pump current inhibitor to the control solution. With the exception of the concentration-dependence studies, the $alpha$ -agonist in test solutions was 10 µM noradrenaline (NA; Sigma), 100 µM phenylephrine (Sigma), 100 µM metaraminol (Sigma), or 100 µM methoxamine (Sigma). The $alpha$ ₁-antagonist was 10 µM prazosin (Pz). The $alpha$ ₂-antagonist was 1 µM yohimbine (Yo). The inhibitor of PKC was 1�5 µM staurosporine (St). The inhibitor of I_p was 0�5 mM strophanthidin (Str). Staurosporine and strophanthidin were prepared as stock solutions by dissolving them in dimethyl sulphoxide (DMSO) and diluting to the final concentrations with external solution before the start of the experiment. The final concentration of DMSO was less than 0�41 %, which does not affect I_p (Gadsby & Nakao, 1989).

Pipette [Na⁺] of 60 mM was used to nearly saturate the intracellular Na⁺-binding sites of the Na⁺-K⁺ pump (Nakao & Gadsby, 1989; Gao et al. 1995). Moreover, this increases our signal-to-noise ratio and minimizes the effect of changes in intracellular [Na⁺] on I_p. The Cs⁺ and TEA⁺ in the pipette solution and Ba²⁺ in the external solution were used to eliminate K⁺ conductance. Cd²⁺ in the external solution was to block Ca²⁺ channel current (carried by Ba²⁺) and the Na⁺-Ca²⁺ exchanger. Propranolol was included in the superfusion solution to block the effects of $beta$ -receptors. Some experiments were performed with 100 mM instead of 60 mM pipette [Na⁺], or 1�4 µM (high calcium) instead of 15 nM Ca²⁺ (low calcium) in the pipette solution, or 15�4 mM external KCl (high [K⁺]_o) instead of 5�4 mM (normal [K⁺]_o ), or 1 mM Str instead of 0�5 mM Str. For the 100 mM pipette [Na⁺], the pipette [K⁺] and [Cs⁺] were each reduced by 20 mM and replaced with [Na⁺]. The high calcium was achieved using 10 mM instead of 1 mM pipette CaCl₂ with 11 mM EGTA (Gao et al. 1992). The high [K⁺]_o was achieved by substituting KCl for external NaCl. The high Str was simply added to the external Tyrode solution.

RESULTS

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Abstract
Introduction
Methods
Results
Discussion
References

NA increases I_p in a concentration-dependent manner

After whole-cell recording was initiated, a period of at least 5 min was required for the pipette and intracellular contents to come to steady state (Gao et al. 1992). When steady state was achieved, 0�5 mM Str was applied and the holding current shifted inward as I_p was blocked (Fig. 1A). This change in holding current was reversed upon washout of Str and was considered to reflect control I_p(con). After washout and once the current had again stabilized, a test concentration of NA was applied. This resulted in a small outward shift in holding current, probably due to activation of I_p. After a new steady state was achieved, 0�5 mM Str was again applied. This application resulted in a larger inward current shift as I_{_p(NA)} was blocked. We examined the concentration dependence of the NA-induced increase in I_p at 0 mV and 15 nM [Ca²⁺]_i. Figure 1A shows four original recordings of holding current in the presence of different concentrations of NA (0�1, 1�0, 5�0 and 10 µM). The records shown in Fig. 1A are the first two applications of Str taken from more complex records involving several interventions (see Fig. 8 or Table 1 for some examples). For all the experiments summarized in Fig. 1B, we were able to determine that the NA effect was reversible and there was no systematic run-down of I_p. In five cells, we extended the period of application of 10 µM NA to approximately 4 min before applying Str. The effect on I_p, however, did not appear to be time dependent, since the mean stimulation (14�5 � 1 %) was not significantly different from that determined with shorter applications (see Fig. 1B). Figure 1B illustrates the mean data from a range of NA concentrations. The continuous line shows the best fit of a standard one-to-one binding curve that was used to estimate the concentration of NA at half-maximal stimulation (K_0�5). Clearly, there are many steps between NA application and I_p stimulation so this is an effective K_0�5 for the stimulation and does not necessarily reflect NA binding. The maximal stimulation of I_p (at 10 µM NA in 8 cells) was 14�4 � 2 %. The K_0�5 for the effect of NA was 219 nM.

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Figure 1. The concentration dependence of NA-mediated stimulation of I_p

A, holding currents measured in different concentrations of NA. The myocyte membrane voltage was held at 0 mV. The control solution was normal Tyrode solution plus Ba²⁺, Cd²⁺ and propranolol (see Methods); the pipette solution contained 15 nM Ca²⁺. In each trace, the upper horizontal line indicates the time of application of the control solution (Control) and the noradrenaline-containing solution (NA). The lower horizontal bars show the time of application of 0�5 mM strophanthidin (Str). In the upper trace, the vertical bar illustrates the measured I_p amplitude. In the 4 experiments shown, the effect of NA was to increase I_p in each cell by approximately the mean values graphed in panel B. B, the concentration-response curve for the stimulation of I_p by NA. The ratios of I_p in NA to those in control solution (I_{_p(NA)}/I_p(con)) were 1�00 � 0�02 in 0�001 µM NA in 7 experiments, 1�05 � 0�03 in 0�1 µM NA in 13 experiments, 1�11 � 0�02 in 1 µM NA in 7 experiments, 1�14 � 0�03 in 5 µM NA in 6 experiments, 1�14 � 0�02 in 10 µM NA in 8 experiments. Error bars indicate S.D.

The increase in I_p is specifically coupled to $alpha$ -adrenergic activation

In most experiments, we measured I_p using 0�5 mM Str, which yields 93-96 % pump inhibition (Gadsby & Nakao, 1989). Thus, one non-specific mechanism of stimulation could be a NA-induced change in affinity of the Na⁺-K⁺ pump for Str, though one would expect such a stimulation to be limited to less than 7 %. Nevertheless, we tested this possibility by increasing the concentration of Str to 1 mM and again determining the effect of 10 µM NA on I_p. Figure 2 illustrates the pump current measured with 0�5 mM and 1 mM Str before and after application of NA. In 1 mM Str the ratio I_p(NA)/I_p(con) was 1�14 � 0�04 (9 cells), which is not significantly different from the I_p(NA)/I_p(con) determined with 0�5 mM Str (1�14 � 0�02, in 8 cells), suggesting that the stimulatory effect of NA on I_p is not caused by a NA-induced change in the affinity of Na⁺-K⁺ pump for Str.

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Figure 2. NA effects on I_p are not due to a change in the affinity of Na⁺-K⁺ pumps for Str

A, in 8 cells we used 0�5 mM Str to inhibit I_p and record control pump current (I_p(con)) and pump current in 10 µM NA (I_{_p(NA)}). The ratio I_{_p(NA)}/I_p(con) was 1�14 � 0�02. B, in 9 cells we used 1�0 mM Str to inhibit I_p and record control pump current (I_p(con)) and pump current in 10 µM NA (I_{_p(NA)}). The ratio I_p(NA)/I_p(con)was 1�14 � 0�04. Thus, the Str-measured stimulation of I_p by NA does not depend on the concentration of Str used to block I_p. This suggests the stimulation is not through an effect of NA on the affinity of the Na⁺-K⁺ pump for Str.

Since I_p is a saturable function of intracellular sodium ([Na⁺]_i), I_p might be influenced by changes in [Na⁺]_i. However, in these experiments we used patch pipettes containing 60 mM [Na⁺], which should nearly saturate the internal Na⁺-binding sites of the Na⁺-K⁺ pump in guinea-pig ventricular myocytes (Nakao & Gadsby, 1989; Gao et al. 1995). This should prevent changes in I_p due to NA-related effects on membrane Na⁺ conductance and consequent changes in [Na⁺]_i. Moreover, insofar as [Na⁺]_i is saturating, the NA effect we observed (I_p stimulation) is not due to an increase in the intracellular Na⁺ affinity of the pump. To verify that our observations are [Na⁺]_i independent, we repeated the 10 µM NA study in five cells with pipette [Na⁺] at 100 mM. In the presence of NA, I_p was increased by 14�4 � 1 %, which is the same result obtained with 60 mM pipette [Na⁺].

I_p is also a saturable function of extracellular potassium ([K⁺]_o) (Nakao et al. 1989; Gao et al. 1995), so I_p might be influenced by local accumulation/depletion of K⁺ in the transverse tubules. Gao et al. (1995) estimated changes in tubular K⁺ to be small, of the order of 0�1 mM, however no direct measurement was made. Hence we tested the possible stimulation of I_p due to a NA-related increase in tubular K⁺. Figure 3 illustrates three typical experiments carried out in 15�4 mM external KCl, which should nearly saturate the external K⁺ site of the Na⁺-K⁺ pump. If a NA-related increase in membrane K⁺ conductance were causing sufficient tubular K⁺ accumulation to stimulate I_p in normal 5�4 mM [K⁺]_o, the higher [K⁺]_o should eliminate the stimulation. However, the mean I_{_p(NA)}/I_p(con)at 15�4 mM [K⁺]_o was 1�06 � 0�02 in 0�1 µM NA (8 cells); 1�11 � 0�02 in 1 µM NA (6 cells); 1�15 � 0�02 in 10 µM NA (6 cells); and the approximate K_0�5 for the effect of NA was 166 nM. These data are not significantly different from those obtained in 5�4 mM [K⁺]_o. We therefore conclude the stimulation of I_p by NA is not due to an increase in tubular K⁺ concentration.

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Figure 3. NA effects on I_p are not due to changes in extracellular K⁺ concentration that might occur in the lumen of the transverse tubular system (T-system)

In Fig. 1, the dependence of I_p on NA was recorded with 5�4 mM [K⁺]_o. If this observed stimulation of I_p was due to NA-related accumulation of K⁺ in the T-system, then increasing [K⁺]_o to 15�4 mM should nearly saturate the K⁺-binding sites and eliminate the stimulation. We examine here, in 15�4 mM [K⁺]_o, the stimulation of I_{_p}by 0�1 µM NA (upper trace), 1�0 µM NA (middle trace) and 10 µM NA (lower trace). The mean I_{_p(NA)}/I_p(con) � S.D. (number of cells) are shown above the NA-stimulated I_p for each concentration of NA. The maximal stimulation is about 15 % with a K_0�5 of approximately 166 µM, which is nearly the same as recorded in 5�4 mM [K⁺]_o. We conclude the effects of NA on I_p are not due to local T-system accumulation of K⁺.

The $alpha$ -induced increase in I_p is [Ca²⁺]_i dependent, voltage independent and mediated by PKC

We have previously reported that [Ca²⁺]_i alters the effect of $beta$ -activation on I_p (Gao et al. 1992). The experiments summarized in Fig. 4 were designed to examine whether the stimulatory effect of $alpha$ -activation on I_p is [Ca²⁺]_i dependent. We buffered [Ca²⁺]_i in the electrode solution to 1�4 µM free [Ca²⁺] (Gao et al. 1992), and recorded the effect of different concentrations of NA on I_p in the same manner as shown in Fig. 1. Figure 4 illustrates the mean data from a range of NA concentrations. The continuous lines show the best fit to the standard one-to-one binding curve at 1�4 µM and 15 nM [Ca²⁺]_i. With 1�4 µM [Ca²⁺]_i, the maximal stimulation of I_p (obtained at 1 µM NA) was 14�7 %. The K_0�5 for the effect was at 3 nM NA. In comparison with data recorded at low [Ca²⁺]_i, the maximal stimulation of I_{_p}by NA was essentially unaltered, but the K_0�5 was significantly reduced and the concentration-response relationship was significantly shifted to the left. These results suggest [Ca²⁺]_i increases the sensitivity of some steps in the cascade leading to stimulation of I_p.

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Figure 4. [Ca²⁺]_i affects the sensitivity of the NA-induced stimulation of I_{_p}but not maximal stimulation

Using the same protocol shown in Fig. 1 but with [Ca²⁺]_i buffered to 1�4 µM, we recorded the stimulation of I_p by 0�001, 0�01, 0�1 and 1�0 µM NA. The mean ratio of I_{_p(NA)}/I_p(con) � S.D. (number of cells) was, respectively, 1�05 � 0�02 (10), 1�10 � 0�03 (8), 1�14 � 0�05 (7) and 1�15 � 0�03 (7). These data are shown as fullcir . For purposes of comparison we also plot the data from Fig. 1 as cir . Based on the curve fit of a single binding site model (continuous lines) the maximal stimulation of I_p in 1�4 µM [Ca²⁺]_i was 14�7 % whereas in 15 nM [Ca²⁺]_i it was 14�4 %, and the K_0�5 in 1�4 µM [Ca²⁺]_i was 3 nM whereas in 15 nM [Ca²⁺]_i the K_0�5 was 219 nM. These data show that the maximal stimulation of 14-15 % is independent of [Ca²⁺]_i but the sensitivity to NA increases nearly two orders of magnitude when [Ca²⁺]_i is high. Moreover, the [Ca²⁺]_i independence of maximal stimulation indicates propranolol has effectively blocked $beta$ -activation, since at 15 nM [Ca²⁺]_i, $beta$ -activation inhibits I_p by about 25 %, whereas at 1�4 µM [Ca²⁺]_i, $beta$ -activation has almost no effect at holding voltage of 0 mV. There is no indication of these $beta$ -mediated effects in the data shown here.

We have previously reported that the effects of $beta$ -adrenergic activation on I_p are voltage dependent (Gao et al. 1996). At low [Ca²⁺]_i, $beta$ -activation inhibits I_p by approximately 25 % at all voltages, whereas at high [Ca²⁺]_i the inhibition is eliminated but the voltage dependence of I_p is shifted by about 30 mV in the negative direction. We therefore examined the voltage dependence of the effects of $alpha$ -activation on I_p when [Ca²⁺]_i is either low (15 nM) or high (1�4 µM). The results are summarized in Fig. 5. The ramp protocol for determining the voltage dependence of I_p is illustrated in Fig. 5A and described in the legend. The left-hand panel of Fig. 5B shows the voltage dependence of I_p when [Ca²⁺]_i is low. The upper squares ( squf ) are maximally NA-stimulated I_p and the lower circles ( fullcir ) are the control pump currents recorded from the same cells. The right-hand panel shows that the ratio of NA-stimulated I_p to control is voltage independent when [Ca²⁺]_i is low. Figure 5C shows the results of the same protocol when [Ca²⁺]_i is high. Again, the ratio is voltage independent. These data suggest $alpha$ -activation by NA does not shift the voltage dependence of I_p.

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Figure 5. The voltage dependence of I_p is not shifted by NA when [Ca²⁺]_i is either high or low

A, the ramp protocol for determining the voltage dependence of I_p. In the upper voltage trace, the cell is held at 0 mV, stepped to +20 mV then ramped to -100 mV in a period of 5 s. The lower trace is the current response to the voltage clamp protocol. The ramp protocol was done in the absence of Str where the response included the background membrane current plus I_p, in the presence of Str where the response was only the background membrane current, and lastly again in the absence of Str to verify the cell was not running down. This protocol was repeated in the presence of NA (10 µM NA in low [Ca²⁺]_i and 1 µM NA in high [Ca²⁺]_i). B, the voltage dependence of I_p is not shifted by NA when [Ca²⁺]_i is low (15 nM). I_p was determined by subtracting the ramp current response in the presence of Str from that in the absence of Str. In each cell, I_p was normalized by its value at 0 mV in the absence of NA (in 5 cells the mean pump current was 170 � 44 pA). This procedure removes cell-to-cell variation in pump density and membrane area. The normalized pump currents were averaged, and in the left-hand panel normalized I_p in the presence of NA is shown by squf , whereas the normalized I_p in the absence of NA (Control) is shown by fullcir . The right-hand panel graphs the ratio of the two curves shown in the left-hand panel. The lack of voltage dependence for the ratio indicates a uniform NA stimulation of about 15 % with no voltage shift. C, the voltage dependence of I_p is not shifted by NA when [Ca²⁺]_i is high (1�4 µM). The data were recorded and analysed as described in B. In 4 cells the mean pump current at 0 mV was 160 � 40 pA. Again, the lack of voltage dependence for the ratio of I_p(NA)/I_p(con) indicates a uniform NA stimulation with no voltage shift.

In a previous study (Gao, Mathias, Cohen, Sun & Baldo, 1997b), we found activation of PKC stimulates I_p in guinea-pig ventricular myocytes. Moreover, for a given stimulation of I_p, the concentration of PKC activator (such as phorbol 12-myristate13-acetate (PMA) and 1,2-dioctanoyl-sn-glycerol (diC8)) depends on [Ca^{+ 2}]_i. The PKC inhibitor, staurosporine, eliminated the effect of PMA or diC8 on I_p. In the present study, we have also found that the concentration of NA required for a given stimulation of I_p is [Ca²⁺]_i dependent. These observations suggest that the $alpha$ -effect on I_p might be mediated by PKC. To test this hypothesis, we used the protocol shown in Fig. 6. I_p was first measured in control conditions as the Str blockable current, then, in the same cell, 10 µM NA was applied and I_p remeasured; lastly, still in the same cell, 1�5 µM St was added to the NA-containing solution and I_p was measured a third time. As seen in the numbers above the currents, in 15 nM [Ca²⁺]_i the ratio of I_{_p(NA)}/I_p(con)was 1�15 � 0�02 whereas the ratio I_p(NA+St)/I_p(con)was 1�00 � 0�04. Similarly, in 1�4 µM [Ca²⁺]_i in 7 cells, the ratio I_{_p(NA)}/I_p(con) was 1�14 � 0�02 but application of staurosporine eliminated the stimulation and I_p(NA+St)/I_p(con) was 1�02 � 0�02_. Thus PKC inhibition eliminated $alpha$ -mediated stimulation of I_p. This result supports the hypothesis that the $alpha$ -mediated effect on I_p is through PKC activation, which depends synergistically on [Ca²⁺]_i.

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Figure 6. The PKC inhibitor staurosporine eliminates the stimulation of I_p by $alpha$ -activation when [Ca²⁺]_i is either high or low

Using 0�5 mM Str, we first measured control I_p(con), then applied 10 µM NA and measured I_{_p(NA)} and finally, with 10 µM NA present, we applied St and measured I_p(NA+St). In the upper trace with [Ca²⁺]_i = 15 nM, in 9 cells we found I_{_p(NA)}/I_p(con) was 1�15 � 0�02 and I_p(NA+St)/I_p(con) was 1�00 � 0�04. In the lower trace with 1�4 µM [Ca²⁺]_i, in 7 cells we found I_{_p(NA)}/I_p(con) was 1�14 � 0�02 and I_p(NA+St)/I_p(con) was 1�02 � 0�02. Thus, the stimulation of I_p by $alpha$ -activation appears to be entirely through PKC activation, which is known to be [Ca²⁺]_i dependent and is therefore the most likely source of the [Ca^{2 +}]_i dependence of the NA effect. Moreover, since the ratio I_p(NA+St)/I_p(con)is essentially unity, these data confirm propranolol is blocking any NA-induced $beta$ -mediated effects (see the legend to Fig. 4), as these are through PKA and not PKC (Gao et al. 1994).

The increase in I_p is coupled to the $alpha$ ₁-receptor subtype

The literature suggests $alpha$ -agonists increase I_p in other cardiac cells via $alpha$ ₁-adrenergic receptors (Shah et al. 1988; Zaza et al. 1990; Ertl et al. 1991; Williamson et al. 1993). However, NA, the natural $alpha$ -agonist, is not selective among the receptor subtypes. Thus, the next set of experiments utilized $alpha$ _1-specific agonists to compare their effects on I_p with that of NA. The $alpha$ ₁-selective adrenergic agonists chosen were phenylephrine (PE), methoxamine (Meth), and metaraminol (Meta). The concentrations selected (100 µM for each) were the highest effective concentrations reported in previous studies. Figure 7 shows three recordings of holding current in the presence of 100 µM PE, Meth, and Meta, respectively. After the measurement of control I_p(con), the application of these selective $alpha$ ₁-adrenergic agonists resulted in an outward shift in holding current similar to that observed with NA. Moreover, after a new steady state was achieved, 0�5 mM Str showed agonist pump current, I_{_p(A)}, was larger than I_p(con), and this increase in I_p reversed upon removal of the agonist. The ratio of agonist (A) to control pump current, I_{_p(A)}/I_p(con), is listed above the agonist in Fig. 7 and the number of cells is in parentheses. The ratio of I_p(recon)/I_p(con) is also listed above the recontrol currents in Fig. 7. We were able to complete the third application of Str in twelve of the thirty-two experiments, hence the number of cells (shown in parentheses) for the recontrol is less than for the agonist, but where the patch lasted long enough to record a recontrol current there was no indication of run-down. With application of any of the three $alpha$ ₁-adrenergic agonists, both the time course of the change in holding current and the increase in I_p are similar to those induced by NA. This implies the $alpha$ -adrenergic stimulatory effect of NA on I_p is mediated via $alpha$ ₁-adrenergic receptors.

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Figure 7. Specific $alpha$ ₁-agonists affect I_p in the same manner as NA

The 3 $alpha$ ₁-specific agonists tested were phenylephrine (100 µM), methoxamine (100 µM) and metaraminal (100 µM). In all experiments [Ca²⁺]_i was 15 nM. In each experiment we measured pump current in control I_p(con) then $alpha$ ₁-agonist I_{_p(A)} and if the patch survived long enough, we remeasured control I_p(recon). Above the traces we list: mean I_{_p(A)}/I_p(con) � S.D. (number of cells). The effects of these $alpha$ -agonists are indistinguishable from those of NA, suggesting NA acts in ventricle through the $alpha$ ₁-receptor subtype.

If the $alpha$ -adrenergic-mediated stimulation of I_p is via $alpha$ ₁-receptors, prazosin (Pz), a selective $alpha$ ₁-receptor antagonist, should completely abolish the effect. As shown in Fig. 8A, 10 µM Pz antagonizes the effects of NA on I_p in low [Ca²⁺]_i and Fig. 8B shows the same result in high [Ca^{+ 2}]_i. We attempted to complete this same protocol with each of the $alpha$ ₁-agonists (Table 1), however, we often lost the patch before the entire protocol could be completed. In all experiments reported in Table 1, we measured control I_p(con), agonist I_{_p(A)} and agonist plus prazosin I_p(A+Pz). In a subset of cells we were able to remeasure agonist I_{_p(A2)} and recontrol I_p(recon). All these results are summarized in Table 1 where mean � S.D. values are given and the number of cells used is in parentheses. Although Pz is selective for $alpha$ ₁-receptors, a sufficiently high concentration might bind to and competitively inhibit $alpha$ ₂-receptors. As a control for this possibility, we used the $alpha$ ₂-antagonist yohimbine (Yo) to demonstrate $alpha$ ₂-receptors are not involved. Figure 8C shows the result in low [Ca²⁺]_i. We did not repeat the study in high [Ca²⁺]_i. The protocol is the same as with Pz except 1 µM Yo is used as the antagonist. The result is quite different, however, as the mean value of I_p(NA+Yo)/I_p(con) is the same as I_{_p(NA)}/I_p(con), so the $alpha$ ₂-antagonist Yo has no significant effect on the NA-mediated stimulation of I_p whereas the $alpha$ ₁-antagonist Pz blocks the effect. These data support the hypothesis that the effects of $alpha$ -activation on I_p are mediated by the $alpha$ ₁-receptor subtype. Moreover, Fig. 8 and Table 1 document that, when the patch is stable, we can reproducibly measure $alpha$ -mediated stimulation of I_p with no detectable run-down.

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Figure 8. The effects of NA on I_p are eliminated by prazosin (Pz), a specific $alpha$ ₁-antagonist, but unaffected by yohimbine (Yo), a specific $alpha$ ₂-antagonist

Above each test condition, we report: mean I_p(test)/I_p(con) � S.D. (number of cells). A, a typical record in low (15 nM) [Ca²⁺]_i. We measured control I_p(con), applied 10 µM NA and measured I_{_p(NA)}, added 10 µM prazosin to the NA-containing solution and measured I_p(NA+Pz), washed out the prazosin and remeasured I_p(NA2), and lastly washed out the NA and remeasured control I_p(recon), In all experiments reported, we measured I_p(con), I_p(NA) and I_P(NA+Pz), but in half of these we lost the patch before obtaining our second measurements I_p(NA2) and I_p(recon). B, the same protocol and information are illustrated except [Ca²⁺]_i was 1�4 µM and NA was 1 µM. C, the protocol is the same as in A, except the $alpha$ ₂-antagonist yohimbine (Yo) is substituted for Pz. However, 1 µM Yo does not antagonize the NA-mediated stimulation of I_p. These data provide direct evidence that the effects of NA on I_p in ventricle are mediated by the $alpha$ ₁-receptor subtype. Moreover, in the 13 cells in which we were able to record I_p(NA2)/I_p(con), the ratio is within 1 % of the initial I_p(NA)/I_p(con), and in the 11 cells in which we completed the protocol, I_p(recon) is within 2 % of the initial I_p(con). Thus, our measurement of the NA effect is reproducible and run-down of I_p is negligible.

Table 1. The $alpha$ ₁-antagonist prazosin (Pz) eliminates the stimulation of I_p by $alpha$ ₁-agonists

$alpha$ -Agonist (A)	I_p(A)/I_p(con)	I_p(A+Pz)/I_p(con)	I_p(A2)/I_p(con)	I_p(recon)/I_p(con)
Phenylephrine, 100 µm	1�14 � 0�04 (6)	1�01 � 0�05 (6)	1�13 � 0�05 (5)	1�02 � 0�03 (5)
Methoxamine, 100 µM	1�13 � 0�03 (17)	0�99 � 0�04 (17)	1�14 � 0�05 (8)	1�00 � 0�03 (8)
Metaraminol, 100 µM	1�15 � 0�05 (8)	1�00 � 0�04 (8)	1�14 � 0�07 (7)	1�02 � 0�02 (7)

The protocol for Table 1 is shown in Fig. 8. All experiments were in 15 nm [Ca²⁺]_i. Numbers are means � S.D. (number of cells).

DISCUSSION

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Abstract
Introduction
Methods
Results
Discussion
References

We have utilized the whole-cell patch-clamp technique to directly measure I_p in isolated guinea-pig ventricular myocytes. We have found the NA-mediated increase in I_p is specifically coupled to activation of $alpha$ ₁-adrenergic receptors. The first evidence for $alpha$ -adrenergic stimulation of cardiac Na⁺-K⁺ pumps was in canine Purkinje myocytes, where Shah et al. (1988) showed the phenylephrine-induced change in holding current is eliminated by dihydro-ouabain. Following this report, Zaza et al. (1990) reported $alpha$ -adrenergic agonists reduce intracellular Na⁺ activity, a finding also consistent with stimulation of Na⁺-K⁺ pumps in Purkinje myocytes. Moreover, in isolated rat papillary muscle, hyperpolarization of the resting membrane potential induced by $alpha$ -agonists was abolished in the presence of ouabain (Ertl et al. 1991), suggesting $alpha$ -activation stimulates the Na⁺-K⁺ pump in this tissue as well. More recently, Williamson et al. (1993) observed an $alpha$ -receptor-mediated increase in holding current in isolated rat ventricular myocytes, and this increase was eliminated by ouabain. The above results indirectly support $alpha$ -adrenergic stimulation of the Na⁺-K⁺ pump. Our results are consistent with the above and more directly implicate the ventricular Na⁺-K⁺ pump as one specific target of $alpha$ ₁-adrenergic activation. Moreover, we have shown direct evidence that all the $alpha$ -effects on the Na⁺-K⁺ pumps are through PKC-mediated phosphorylation.

At present, the steps in signal transfer between ventricular $alpha$ ₁-receptor activation and pump stimulation are largely unknown. In Purkinje myocytes the first step appears to involve a pertussis toxin-sensitive G_q protein (Shah et al. 1988). Several studies in cardiac myocytes have suggested PKC is responsible for many events caused by activation of $alpha$ ₁-adrenergic receptors; for example, stimulation of the chloride conductance (Walsh, 1991; Ackerman & Clapham, 1993), K⁺ current (Tohse et al. 1987b; Tohse, Nakaya & Kanno, 1992), or Na⁺-H⁺ antiport (Gambassi, Spurgeon, Lakatta, Blank & Capogrossi, 1992). In ventricular myocytes, Gao et al. (1997b) show that PMA, a synthetic PKC activator, increases I_p, and its effect depends on [Ca²⁺]_i, but staurosporine, a potent PKC inhibitor, eliminates the effect. These data suggest PMA and free [Ca²⁺]_i act synergistically to activate PKC and increase I_p. In the present study, $alpha$ ₁-adrenergic activation, like PMA, increases I_p,depends on free [Ca²⁺]_i and its stimulatory effect on I_p is eliminated by staurosporine. This strongly suggests the effect of $alpha$ ₁-adrenergic activation on I_p is also mediated by activation of PKC. In general, $alpha$ ₁-receptors are thought to be coupled through a G protein to activate phospholipase C (PLC), which cleaves phosphatidylinositol 4,5-diphosphate into inositol 1,4,5-trisphosphate (InsP₃) and diacylglycerol (DAG). InsP₃ causes the release of intracellular Ca²⁺, then [Ca²⁺]_i and DAG synergistically activate PKC (Nosek, Williams, Zeigler & Godt, 1986; Berridge, 1987, 1993; Scholz et al. 1988; Okumura, Kawai, Hashimoto, Ito, Ogawa & Satake, 1988). At the molecular level, PKC is thought to be activated when it associates with the plasma membrane and contacts DAG. Intracellular Ca²⁺ binds to PKC and helps anchor it to the membrane, hence a lower concentration of membrane DAG can activate PKC when [Ca²⁺]_i is elevated (Berridge, 1987; Mosior & McLaughlin, 1991).

The above summary provides the following hypothesis for our results: in cardiac ventricular myocytes, activation of $alpha$ ₁-adrenergic receptors is coupled, probably via a G protein as in other cells, to activation of PLC, which cleaves phosphatidylinositol to InsP₃ and DAG. In the whole-cell patch-clamp mode, [Ca²⁺]_i is strongly buffered by 11 mM EGTA, thus InsP₃ has little effect on [Ca²⁺]_i but DAG activates membrane-associated PKC. When we buffer [Ca²⁺]_i to 1�4 µM, the association of PKC with the plasma membrane is stabilized and a relatively low membrane concentration of DAG can activate all the available PKC. As a consequence, activation of a small fraction of the $alpha$ ₁-receptors by NA is sufficient to generate enough DAG to fully activate PKC. When we buffer [Ca²⁺]_i to 15 nM, the association of PKC with the plasma membrane is much briefer, hence a relatively high membrane concentration of DAG is required to fully activate PKC. In this situation, a much higher concentration of NA is needed to activate a larger fraction of the $alpha$ -receptors, G proteins and PLC to generate sufficient DAG to fully activate PKC. In this model, the [Ca²⁺]_i-dependent saturable step is activation of PKC. Activation of PKC results in phosphorylation of target proteins, perhaps the Na-K⁺ pumps or perhaps a regulatory protein of the Na⁺-K⁺ pumps. In either event, PKC mediated phosphorylation leads to an increase in the maximal rate of Na⁺-K⁺-ATPase activity.

Although this model is consistent with the results of the present study and conforms with the conventional view of PKC activation, there are some questions that need to be addressed. First, Gao et al. (1997b) found PMA stimulates I_p by about 30 %, which is twice the stimulation elicited by $alpha$ -activation, yet the PMA stimulation was eliminated by staurosporine, suggesting it was entirely mediated by PKC. It is possible that PMA has a larger effect on the maximal kinase activity of PKC, or that PMA has access to a pool of PKC inaccessible to DAG. Otherwise, this observation appears inconsistent with PKC activation being the saturable step in the experiments reported here. Second, Chibalin, Vasilets, Hennekes, Pralong & Geering (1992) have shown PKC phosphorylates the $alpha$ -subunit of the Na⁺-K⁺ pump, and Vasilets & Schwartz (1992), as well as Bertorello, Aperia, Walaas, Nairn & Greengard (1991) found in vitro PKC-mediated phosphorylation of the Na⁺-K⁺pump inhibits ATPase activity whereas in ventricular myocytes, we find PKC activation stimulates ATPase activity. In ventricular myocytes, the steps following activation of PKC are apparently more complex than simple, direct phosphorylation of the pump.

Regardless of the details of the pathway, the coupling of ventricular $alpha$ ₁-activation to an increase in I_{_p}is physiologically important. With sympathetic activation, NA levels in the heart increase to values in the range of the [NA]-I_p response curves shown in Fig. 4 (Fujii & Vatner, 1986). As a result of sympathetic activity, heart rate increases, plateau Ca²⁺ current increases and diastolic interval decreases. The increase in rate is due to sympathetic effects on pacemaker cells. So in ventricular cells, sympathetic effects are to increase the plateau Ca²⁺ current, which tends to prolong action-potential duration, so outward currents are also increased to maintain a more constant duration of the action potential and allow a sufficient diastolic interval to fill the ventricles. To understand how the Na⁺-K⁺ pump contributes to these results, one has to consider the effects of both $beta$ - and $alpha$ -adrenergic activation, their relation to [Ca²⁺]_i and [Na⁺]_i, and the relation of [Ca²⁺]_i to the $alpha$ - and $beta$ -mediated effects on I_p. This is obviously a complex system capable of a variety of responses to differing conditions. However, one can hypothesize an idealized situation in which there is a relatively low initial [Ca²⁺]_i and sympathetic activation saturates both $alpha$ - and $beta$ -receptors. When sympathetic tone increases, initially $alpha$ - and $beta$ -effects on I_p are antagonistic and there is little change in I_p; however, the increases in Ca²⁺ current and heart rate cause a rise in [Ca²⁺]_i and [Na⁺]_i. The increase in [Na⁺]_i causes a steady-state increase in I_p, moreover the increase in [Ca²⁺]_i causes both $beta$ - and $alpha$ -activation to synergistically further increase I_p. Since I_p is a net outward current, its increase tends to shorten the action potential and prevent an excessively short diastolic interval. Lastly, the increased I_p is a negative feedback brake on the increase in [Na⁺]_i as well as [Ca²⁺]_i, which depends on Na⁺-Ca²⁺ exchange. The role of the Na⁺-K⁺ pump in controlling intracellular ion concentrations is widely appreciated; however, its role as a major current shaping the cardiac action potential is not so widely discussed. In these guinea-pig ventricular myocytes, the action potential plateau repolarizes at a rate of about 85 mV s^-1 and a typical cell has a capacitance of 180 pF, which gives a net outward current during the plateau of 15 pA. The total Na⁺-K⁺ pump current under normal physiological conditions and at 0 mV is on average about 100 pA, so a 15 % increase in I_p will double the rate of repolarization if all else remains equal. Thus, $alpha$ - and $beta$ -mediated changes in I_p should have significant effects on the action potential shape and duration.

REFERENCES

Top
Abstract
Introduction
Methods
Results
Discussion
References

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[Abstract]

Acknowledgements

This work was supported by grants HL 28958, HL 54031 and HL 20558 from the National Heart, Lung and Blood Institute.

Corresponding author

R. T. Mathias: Department of Physiology & Biophysics at the State University of New York at Stony Brook, Stony Brook, NY 11794-8661, USA.

Email: rtmathias{at}physiology.pnb.sunysb.edu

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				A. G. Therien and R. Blostein Mechanisms of sodium pump regulation Am J Physiol Cell Physiol, September 1, 2000; 279(3): C541 - C566. [Abstract] [Full Text] [PDF]

				R. T. Mathias, I. S. Cohen, J. Gao, and Y. Wang Isoform-Specific Regulation of the Na+-K+ Pump in Heart Physiology, August 1, 2000; 15(4): 176 - 180. [Abstract] [Full Text] [PDF]

				E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]

				J.-D. Horisberger and S. Kharoubi-Hess Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes J. Physiol., March 15, 2002; 539(3): 669 - 680. [Abstract] [Full Text] [PDF]