Role of domain I of neuronal Ca2+ channel alpha1 subunits in G protein modulation

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Role of domain I of neuronal Ca²⁺ channel $alpha$ 1 subunits in G protein modulation

Gary J. Stephens, Carles Cantí, Karen M. Page and Annette C. Dolphin

Department of Pharmacology, University College London, London WC1E 6BT, UK

Received 11 February 1998; accepted 30 March 1998.

ABSTRACT

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Abstract
Introduction
Methods
Results
Discussion
References

We studied the G protein inhibition of heteromultimeric neuronal Ca²⁺ channels by constructing a series of chimeric channels between the strongly modulated $alpha$ 1B subunit and the $alpha$ 1E(rbEII) subunit, which showed no modulation.

In parallel studies, $alpha$ 1 subunit constructs were co-expressed together with the accessory Ca²⁺ channel $alpha$ 2- $delta$ and $beta$ 2a subunits in mammalian (COS-7) cells and Xenopus oocytes. G protein inhibition of expressed Ca²⁺ channel currents was induced by co-transfection of G $beta$ 1 and G $gamma$ 2 subunits in COS-7 cells or activation of co-expressed dopamine (D2) receptors by quinpirole (100 nM) in oocytes.

The data indicate that transfer of the $alpha$ 1B region containing the N-terminal, domain I and the I-II loop (i.e. the $alpha$ 1B_1-483 sequence), conferred G protein modulation on $alpha$ 1E(rbEII), both in terms of a slowing of activation kinetics and a reduction in current amplitude.

In contrast, the data are not consistent with the I-II loop and/or the C-terminal forming a unique site for G protein modulation.

INTRODUCTION

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Abstract
Introduction
Methods
Results
Discussion
References

G protein inhibition of neuronal N ( $alpha$ 1B) and P/Q type ( $alpha$ 1A) Ca²⁺ currents is mediated by G $beta$ $gamma$ subunits (Herlitze, Garcia, Mackie, Hille, Scheuer & Catterall, 1996; Ikeda, 1996). Both the intracellular loop that links Ca²⁺ channel transmembrane domains I and II (DeWaard, Liu, Walker, Scott, Gurnett & Campbell, 1997; Zamponi, Bourinet, Nelson, Nargeot & Snutch, 1997) and a C-terminal sequence (Qin, Platano, Olcese, Stefani & Birnbaumer, 1997) have been implicated as sites at which G $beta$ $gamma$ subunits bind to $alpha$ 1 subunits.

Functionally, the site of G protein action remains controversial. Mutations within the I-II loop, and specifically to the arginine residue in a QxxER consensus sequence proposed to be involved in G $beta$ $gamma$ binding (Chen et al. 1995), abolish G $beta$ $gamma$ binding and prevent the slowing of activation induced by GTP $gamma$ S (De Waard et al. 1997). In contrast, the same mutation actually enhanced modulation in a different study (Herlitze, Hockerman, Scheuer & Catterall, 1997); whereas conversion of the entire $alpha$ 1A consensus sequence (QIEER) to that in $alpha$ 1C (QQLEE) did attenuate modulation. Transfer of the IS6/I-II loop from $alpha$ 1B to the non-modulated $alpha$ 1E(rbEII) causes some slowing of current activation kinetics in the presence of GTP $gamma$ S, but does not result in current amplitude modulation (Page, Stephens, Berrow & Dolphin, 1997). In contrast, $alpha$ 1B was reported to retain G protein sensitivity when its I-II loop was replaced by the corresponding sequence from non-modulated $alpha$ 1C (Zhang, Ellinor, Aldrich & Tsien, 1996); their study implicated a role of domain I together with the C-terminal in G protein modulation. However, the G protein inhibition of human $alpha$ 1E appears to be due to G $beta$ $gamma$ binding solely at the C-terminal site (Qin et al. 1997).

Here, we examine the potential contribution of regions implicated in G protein modulation using a series of constructs between the strongly modulated $alpha$ 1B subunit and the rat $alpha$ 1E(rbEII) subunit (Soong, Stea, Hodson, Dubel, Vincent & Snutch, 1993), which shows no modulation (Bourinet, Soong, Stea & Snutch, 1996a; Page et al. 1997). The results suggest that the $alpha$ 1B_1-483 sequence contains important determinants of G protein modulation.

METHODS

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Abstract
Introduction
Methods
Results
Discussion
References

Materials

The following cDNAs were used: rat $alpha$ 1E (rbEII, GenBank accession number L15453); rabbit $alpha$ 1B (D14157); rat $beta$ 2a (M80545); rat $alpha$ 2- $delta$ (M86621); rat D2_long receptor (X77458, N5 rarrow G); bovine G $beta$ 1 (M13236), bovine G $gamma$ 2 (M37183) and Mut3-Green fluorescent protein (GFP) (U73901).

Production of Ca²⁺ channel constructs

Individual constructs were produced by the polymerase chain reaction (PCR) methodology as detailed previously (Page et al. 1997), using lower case letters for C termini and I-II loops and upper case for each of the four transmembrane domains as follows.

$alpha$ 1EbEEE. The pMT2 forward primer, pMT2F (AGC TTG AGG TGT GGC AGG CTT) and the chimeric reverse primer, TCC TGA GAG CAC ACC CAG GAC AAG GTT G, were used with the $alpha$ 1E(rbEII) template; the resulting fragment was used as a primer and extended on the $alpha$ 1EBE1-pMT2 template using the reverse primer, GAC TTC ATG GAG CTC ATC AAG G. The product was digested with Xba I and Acc B7I and subcloned into the corresponding region of the $alpha$ 1E(rbEII)-pMT2 vector. The $alpha$ 1EbEEE construct differs from the chimera (termed EBE) used previously (Page et al. 1997); $alpha$ 1EbEEE substitutes only the I-II loop, whilst EBE exchanged both the 1S6 region and I-II loop.

$alpha$ 1EbEEEb and $alpha$ 1EEEEb. An Xho I site was removed from position 5433 of $alpha$ 1B using the forward primer, AAG TGC CCT GCA CGA GTC GCG TA and the reverse primer, GCA CTC GAG CGC GGA AGA TGA AGC. The product was extended on the $alpha$ 1B-pMT2 template using the forward primer, TTA CTC GAG ACT CTT CCA TCT TAG G, to introduce the Xho I site. This product was digested and subcloned into $alpha$ 1E(rbEII) to give $alpha$ 1EEEEb, and into $alpha$ 1EbEEE to give $alpha$ 1EbEEEb.

$alpha$ 1BbEEE and $alpha$ 1BbEEEb. A Mfe I (pMT2) to Kpn I $alpha$ 1B digestion was used to swap the first domain of $alpha$ 1EbEEE with that of $alpha$ 1B to make $alpha$ 1BbEEE. $alpha$ 1BbEEEb was made by using the same Kpn I site in $alpha$ 1EbEEEb.

PCR was carried out using the proof-reading enzyme, Pfu (Stratagene). The sequences of the sub-cloned PCR products were verified by cycle-sequencing using SequiTherm Excel^TM II (Epicentre Technologies, Madison, USA).

Expression of constructs

COS-7 cells. Cells were transfected by electroporation as described (Campbell, Berrow, Brickley, Page, Wade & Dolphin, 1995). Fifteen, 5, 5 and 1 µg of the pMT2- $alpha$ 1, $alpha$ 2- $delta$ , $beta$ 2a or $beta$ 1b and green fluorescent protein (GFP) constructs, respectively, were used for transfection. When used, G $beta$ 1 and G $gamma$ 2 were included at 2�5 µg each. Cells were maintained at 37�C, then replated and maintained at 25�C prior to recording.

Xenopus oocytes. Adult Xenopus laevis females were anaesthetized by immersion in 0�2 % tricaine then killed by decapitation and pithing, oocytes were surgically removed and defolliculated with 2 mg ml^-1 collagenase type Ia in a Ca²⁺-free ND96 saline (containing (mM): NaCl, 96; KCl, 2; MgCl₂, 1; Hepes, 5; pH adjusted to 7�4 with NaOH) for 2 h at 21�C. cDNAs for the different $alpha$ 1, $beta$ 2a and $alpha$ 2- $delta$ subunits and D2 receptors were co-injected at a ratio of 3:1:1: 3 into the nuclei of stage V and VI oocytes using a Drummond microinjector. Oocytes were incubated at 18�C for 3-7 days in ND96 saline (as above plus 1�8 mM CaCl₂) supplemented with 100 µg ml^-1 penicillin and 100 i.u. ml^-1 streptomycin (Gibco) and 2�5 mM sodium pyruvate.

Electrophysiology

COS-7 cells. Recordings were made from fluorescent cells expressing the GFP reporter gene, replated between 1 and 16 h previously, using a non-enzymatic cell dissociation medium (Sigma). Borosilicate glass electrodes of resistance 2-4 M $Omega$ were filled with a solution containing (mM): caesium aspartate, 140; EGTA, 5; MgCl₂, 2; CaCl₂, 0�1; K₂ATP, 2; Hepes, 10; pH 7�2; osmolarity adjusted to 310 mosmol l^-1 with sucrose. GDP $beta$ S (2 mM) was included where stated. The external solution contained (mM): tetraethylammonium (TEA) bromide, 160; KCl, 3; NaHCO₃, 1�0; MgCl₂, 1�0; Hepes, 10; glucose, 4; BaCl₂, 1; pH 7�4; osmolarity adjusted to 320 mosmol l^-1 with sucrose.

Whole cell currents were recorded using an Axopatch 1D amplifier. Data were filtered at 2 kHz and digitized at 5-10 kHz and analysed using pCLAMP6 and Origin 3.5. The junction potential between external and internal solutions was 6 mV, the values given in the figures and text have not been corrected for this. Current records are shown following leak and capacitance current subtraction (P/4 or P/8 protocol) and series resistance compensation up to 85 %.

Xenopus oocytes. Whole cell recordings from oocytes were made in the two-electrode voltage clamp configuration with a chloride-free solution containing (mM): Ba(OH)₂, 40; TEA-OH, 50; KOH, 2; niflumic acid, 0�4; Hepes, 5; pH adjusted to 7�4 with methanesulphonic acid). In some experiments, niflumic acid was omitted and oocytes injected with 30-40 nl of 100 mM BAPTA to suppress endogenous chloride currents. Data were filtered at 1 kHz using a Geneclamp 500 amplifier, digitized through a Digidata 1200 interface (Axon Instruments) and stored using data acquisition software pCLAMP6. Currents were leak subtraction on line (P/4 protocol)

Experiments were performed at room temperature (20-24�C). Data are expressed as means � S.E.M. Statistical analysis was performed using Student's paired or unpaired t test as appropriate.

RESULTS

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Abstract
Introduction
Methods
Results
Discussion
References

Effect of G $beta$ 1 $gamma$ 2 co-expression on Ca²⁺ channel constructs

A series of chimeras between Ca²⁺ channel $alpha$ 1B and $alpha$ 1E(rbEII) subunits was constructed as shown in Fig. 1A to investigate the role of domain I, the I-II loop and the C-terminal in G protein regulation. $alpha$ 1 subunits were co-expressed together with accessory $alpha$ 2- $delta$ and $beta$ 2a subunits in COS-7 cells and modulation was studied by co-expressing G $beta$ 1 $gamma$ 2 subunits. In controls, G $beta$ 1 $gamma$ 2 was replaced by pMT2 vector and 2 mM GDP $beta$ S was included in the patch pipette to limit tonic facilitation (Stephens, Brice, Berrow & Dolphin, 1998). Current-voltage profiles were constructed (Fig. 1B) and the ability of co-expressed G $beta$ 1 $gamma$ 2 subunits to slow current activation, a characteristic of G protein inhibition, was examined. Time constants of activation ( $tau$ _act) were derived from single exponential fits to the rising phase of currents (Fig. 1C). In the absence of exogenous G $beta$ $gamma$ subunits, all currents activated rapidly and showed little inactivation over the time course used (as expected for co-expression of the $beta$ 2a subunit which retards voltage-dependent inactivation of Ca²⁺ channels (Olcese et al. 1994)). In the presence of G $beta$ 1 $gamma$ 2, $alpha$ 1B currents showed a marked slowing of activation kinetics in comparison to controls; in contrast, $alpha$ 1E(rbEII) currents showed no difference in activation with or without G $beta$ 1 $gamma$ 2. Of the other constructs, those containing the N-terminal sequence/domain I/I-II loop of the $alpha$ 1B subunit sequence ( $alpha$ 1B_1-483) exhibited currents showing clear kinetic slowing in the presence of G $beta$ 1 $gamma$ 2. Time constants of activation showed no significant differences for the effects of G $beta$ 1 $gamma$ 2 on these responsive constructs: at -10 mV $tau$ _act values were 32 � 9 ms ( $alpha$ 1B, n = 8), 29 � 6 ms ( $alpha$ 1BbEEE, n = 11) and 26 � 5 ms ( $alpha$ 1BbEEEb, n = 7). Thus, the subsequent exchange of the C-terminal, in addition to the $alpha$ 1B_1-483 sequence, had no further effect on current activation kinetics.

Constructs in which either the I-II loop alone ( $alpha$ 1EbEEE), the C-terminal sequence alone ( $alpha$ 1EEEEb) or both elements ( $alpha$ 1EbEEEb) were exchanged exhibited currents showing no significant slowing in activation kinetics in the presence of G $beta$ 1 $gamma$ 2 (Figs 1B and C).

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Figure 1. Effect of G $beta$ 1 $gamma$ 2 on Ca²⁺ channel constructs in COS-7 cells

Ca²⁺ channel constructs between $alpha$ 1B and $alpha$ 1E(rbEII) subunits (as shown in A) were transfected together with cDNA coding for $alpha$ 2- $delta$ and $beta$ 2a subunits. B, example current density-voltage profiles for control cells (in the presence of GDP $beta$ S) and in the presence of G $beta$ 1 $gamma$ 2. The initial test potential (V_t) shown was always -50 mV and was increased in 10 mV increments; holding potential V_H = -100 mV. Values for scale bars on the left apply also to scale bars on the right. C, time constant of activation ( $tau$ _act) at -10 mV for Ca²⁺ channel constructs coexpressed with G $beta$ 1 $gamma$ 2 (black columns) or in control conditions in the presence of GDP $beta$ S (open columns); number of experiments, n, is given in parentheses. Only currents resulting from constructs containing the $alpha$ 1B_1-483 sequence showed a clear slowing of activation kinetics.

Receptor-mediated G protein inhibition of Ca²⁺ channel constructs

In parallel studies, we reconstructed receptor-mediated inhibition of constructs (together with $alpha$ 2- $delta$ and $beta$ 2a subunits) in Xenopus oocytes. Inhibitory coupling of dopamine (D2) receptors was assessed in terms of the reduction in current amplitude by a saturating concentration of quinpirole (100 nM) (Fig. 2A). Only constructs containing the $alpha$ 1B_1-483 sequence were modulated by receptor activation. There were no significant differences in inhibition by quinpirole amongst the responsive chimeras; however, quinpirole did cause a higher percentage inhibition in $alpha$ 1B than in $alpha$ 1BbEEE (P < 0�005) or $alpha$ 1BbEEEb (P < 0�005). No additional modulation to that seen in $alpha$ 1BbEEE was apparent in $alpha$ 1BbEEEb. Inhibition by quinpirole was absent in all of the other chimeras.

Quinpirole-induced inhibition in constructs containing the $alpha$ 1B_1-483 sequence was accompanied by a depolarizing shift in the midpoint of activation (V_�) of current-voltage curves (Fig. 2B). Modified Boltzmann functions fitted to the data shown gave similar shifts in V_�with quinpirole: $alpha$ 1B, +7�4 mV; $alpha$ 1BbEEE, +5�6 mV; $alpha$ 1BbEEEb, +6�5 mV. No such shift was seen for $alpha$ 1E(rbEII) (Fig. 2B).

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Figure 2. Effect of activation of D2 receptors on Ca²⁺ channel constructs in Xenopus oocytes

Ca²⁺ channel constructs were injected together with cDNA coding for $alpha$ 2- $delta$ and $beta$ 2a subunits and dopamine (D2) receptors in all cases. A, activation of D2 receptors by quinpirole caused an inhibition of currents formed by constructs containing the $alpha$ 1B_1-483 sequence; V_t = 0 mV and V_H = -100 mV; number of experiments, n, is given in parentheses. B, time course of barium current (I_Ba) inhibition by quinpirole (Q, 100 nM) in selected constructs (V_t = 0 mV, left panel) and corresponding effects of quinpirole on the current-voltage relationship (right panel). Current-voltage data were fitted with the equation: Current = G_max(V - V_rev)/1 + exp[(V - V_�)/k]},where G_maxis maximum slope conductance, V_� is the voltage at which 50 % of the current is activated, V_revis the null potential and k is the slope factor. Quinpirole inhibition ( fullcir ) was accompanied by a reduction in current amplitude, particularly at hyperpolarized potentials, with a corresponding shift in V_� values.

Effects of facilitating prepulses on G $beta$ 1 $gamma$ 2-induced inhibition on Ca²⁺ channel constructs

The voltage-dependent G protein modulation of Ca²⁺ channels can be reversed by the application of a large depolarizing prepulse prior to an activating pulse. Figure 3A illustrates that a depolarizing prepulse reversed both the inhibition of current amplitude and the slowed activation kinetics induced by G $beta$ 1 $gamma$ 2 overexpression in COS cells expressing constructs containing the $alpha$ 1B_1-483 sequence. G $beta$ 1 $gamma$ 2-induced slowing of activation kinetics was maximal at just supra-threshold potentials and was reversed by prepulses to the control levels observed in the presence of GDP $beta$ S (Fig. 3B). The degree of prepulse-induced current amplitude facilitation (P2 : P1) in the presence of G $beta$ 1 $gamma$ 2 was also maximal over a similar voltage range in responsive constructs (Fig. 3C). No facilitation was observed in constructs lacking the first domain of $alpha$ 1B.

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Figure 3. Reversal of G $beta$ $gamma$ inhibition of Ca²⁺ channel constructs by large depolarizing prepulses in COS-7 cells

Application of depolarizing prepulses reversed G $beta$ 1 $gamma$ 2-induced inhibition, as shown for selected constructs. A, I_Ba (V_t = -40 to 0 mV) was examined immediately before (P1) and 10 ms after (P2) application of a depolarizing prepulse to +120 mV; V_H = -100 mV. B, in constructs containing the $alpha$ 1B_1-483 sequence, prepulses (pp) reversed G $beta$ 1 $gamma$ 2-induced slowing of activation kinetics to control levels (recorded separately at P1 in control cells). C, in constructs containing the $alpha$ 1B_1-483 sequence, prepulses reversed G $beta$ 1 $gamma$ 2-induced inhibition of current amplitude (P2 : P1 measured at 50 ms). In all instances, note the lack of effects on $alpha$ 1E(rbEII). Number of experiments, n, is given in parentheses.

Prepulses also partially reversed the quinpirole-induced inhibition of current amplitude in constructs containing the $alpha$ 1B_1-483 sequence in oocytes (Fig. 4A and B). An incomplete reversal of inhibition by a large depolarizing prepulse is a characteristic of G protein inhibition and might be indicative of additional non-voltage-dependent mechanisms (Luebke & Dunlap, 1994) or due partially to the rebinding of G $beta$ $gamma$ subunits during the 10 ms interpulse interval. Prepulses caused a significant facilitation of quinpirole-inhibited currents in comparison to control levels (prepulses applied in the absence of quinpirole) for responsive constructs (Fig. 4A). There was no clear difference in the percentage facilitation between responsive constructs. No facilitation was observed in constructs lacking the first domain of $alpha$ 1B.

Therefore, the transfer of the $alpha$ 1B_1-483 sequence conferred full G protein modulation onto $alpha$ 1E(rbEII) which was reversed by depolarizing prepulses. No additional effects were seen with the subsequent exchange of the C-terminal sequence.

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Figure 4. Reversal of D2 receptor-induced inhibition of Ca²⁺ channel constructs by large depolarizing prepulses in Xenopus oocytes

Application of depolarizing prepulses caused a reversal of quinpirole-induced inhibition of I_Ba for constructs containing the $alpha$ 1B_1-483 sequence. A, percentage facilitation of control (open columns) and quinpirole-inhibited I_Ba (black columns) induced by a prepulse to +100 mV. B, in constructs containing the $alpha$ 1B_1-483 sequence, inhibition of control I_Ba (1) by quinpirole (2) was partially reversed by prepulse to +100 mV (3). In all cases, V_t = 0 mV and V_H = -100 mV. Number of experiments, n, is given in parentheses.1

DISCUSSION

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Abstract
Introduction
Methods
Results
Discussion
References

Taking clues from our previous studies and those of others, we constructed chimeric channels to study the role of several regions of the Ca²⁺ channel $alpha$ 1 subunit implicated in G protein regulation. The data indicate that the $alpha$ 1B_1-483 sequence, representing the N-terminal, domain I and the I-II loop, contain important determinants. In contrast, the data are not consistent with the I-II loop and/or the C-terminal alone forming a unique site for G protein modulation.

Role of the I-II loop in G protein inhibition

Despite data demonstrating binding of radiolabelled G $beta$ $gamma$ to the Ca²⁺ channel I-II loop (De Waard et al. 1997; Zamponi et al. 1997; Qin et al. 1997), the functional importance of this site is still controversial (see Dolphin, 1998). Substitution of the I-II loop of $alpha$ 1C, which does not bind G $beta$ $gamma$ (De Waard et al. 1997; Zamponi et al. 1997), into $alpha$ 1B (Zhang et al. 1996) or $alpha$ 1E (Qin et al. 1997) produces constructs that retain G protein sensitivity. However, opposite results have also been reported (Herlitze et al. 1997); conversion of the G $beta$ $gamma$ -binding QxxER consensus sequence in the I-II loop of $alpha$ 1A subunit to the corresponding $alpha$ 1C sequence greatly reduced G protein inhibition. Importantly, this conversion did not completely abolish inhibition (Herlitze et al. 1997). Furthermore, whilst replacement of the I-II loop of $alpha$ 1A with that of $alpha$ 1B did increase G protein inhibition, it did not fully account for all of the inhibition seen in parental $alpha$ 1B (Zamponi et al. 1997). These findings suggest that molecular determinants for G protein modulation additional to the I-II loop are likely to exist. In support of this, we have demonstrated that a chimera in which the IS6/I-II loop of $alpha$ 1E(rbEII) was replaced by that of $alpha$ 1B (termed EBE) exhibited a greater slowing of current activation kinetics with GTP $gamma$ S than did $alpha$ 1E(rbEII), but that GTP $gamma$ S had no effects on current amplitude (Page et al. 1997).

In the present study, the $alpha$ 1EbEEE construct, in which only the I-II loop and not IS6 was exchanged, was examined. In agreement with our previous study, G proteins had no effect on current amplitude. However, no significant changes in activation kinetics were seen. In our previous study the $beta$ subunit used was $beta$ 1b, which produces less antagonism of G $beta$ $gamma$ than the $beta$ 2a subunit used here (Qin et al. 1997). However, it appears that the additional substitution of the 1S6 region of transmembrane domain I, which has been implicated as a determinant of voltage-dependent Ca²⁺ channel inactivation (Zhang, Ellinor, Aldrich & Tsien, 1994), can also subtly affect activation kinetics in the presence of G $beta$ $gamma$ subunits.

Role of the intracellular C-terminal tail in G protein inhibition

The Ca²⁺ channel C-terminal has been implicated in G protein inhibition. Zhang et al. (1996) propose that both domain I and the C-terminal contribute elements to a multi-structural site, whilst Qin et al. (1997) suggest a unique site on the C-terminal. The thirty-eight amino acid G $beta$ $gamma$ -binding site identified in the human $alpha$ 1E C-terminal sequence (Qin et al. 1997) is entirely conserved in $alpha$ 1E(rbEII). Despite the presence of this site, we saw no evidence of inhibition of $alpha$ 1E(rbEII). A possible explanation for this is that $beta$ subunits may compete for G $beta$ $gamma$ binding and effectively block any modulation. More specifically, the $beta$ 2a subunit presence here was shown selectively to block G $beta$ $gamma$ binding to the human $alpha$ 1E C-terminal site (Qin et al. 1997). However, this is unlikely to explain the lack of G protein effects here as we also see no receptor-mediated inhibition of $alpha$ 1E(rbEII) in the absence of any exogenous $beta$ subunits (C. Cantí and A. C. Dolphin, unpublished results), suggesting an inherent G protein insensitivity of the $alpha$ 1E(rbEII) subunit.

The $alpha$ 1B_1-483 sequence contributes to G protein inhibition

Transfer of the $alpha$ 1B_1-483 sequence to $alpha$ 1E(rbEII) conferred G protein-induced slowing of current activation kinetics and reduction in current amplitude. In another major study on the determinants of G protein modulation, Zhang et al. (1996) proposed a role for domain I together with the C-terminal, but not the I-II loop. In contrast, we find that for $alpha$ 1E(rbEII), inhibition was not further increased by the subsequent exchange of the C-terminal in combination with the $alpha$ 1B_1-483 sequence; this suggests that $alpha$ 1E(rbEII) lacks only molecular determinants within the $alpha$ 1B_1-483 sequence. Despite these discrepancies, it is clear that domain I contains important determinants of G protein modulation. In this regard, the reduction in G protein inhibition caused by exchanging both domain I and the C terminal of $alpha$ 1B for these regions of $alpha$ 1C (Zhang et al. 1996) may be interpreted not as a lack of G $beta$ $gamma$ binding to the $alpha$ 1B I-II loop, but rather a failure of binding to be fully translated into a functional effect due to the lack of the $alpha$ 1B domain I. Such an effect is consistent with the present results (as discussed below).

The $alpha$ 1B_1-483 sequence represents the N-terminal, domain I and the I-II loop regions. The corresponding sequence of $alpha$ 1E(rbEII) shows only a few major regions of difference. The N-terminal shows the clearest difference, with the $alpha$ 1E(rbEII) N-terminal being fifty-five amino acids shorter than that of $alpha$ 1B. The involvement of the N-terminal region is currently under investigation. The entire 1S1-1S6 region shows a remarkable degree of homology (including complete conservation of the intracellular loops between IS2-IS3 and IS4-IS5); only the H5 linker between IS5 and IS6 in the putative pore region shows a clear divergence. However, this more likely reflects differences in pore properties such as ion permeation (Bourinet et al. 1996b) and single channel conductance (Dirksen, Nakai, Gonzalez, Imoto & Beam, 1997). Finally, the I-II loop shows sequence differences. However, as discussed previously (Page et al. 1997) and above, the I-II loop alone does not account for G protein inhibition.

Our findings suggest that the binding of G $beta$ $gamma$ to either the I-II loop or the C-terminal alone is insufficient to mediate G protein inhibition. However, the data do not rule out a contribution of either region to a site composed of different elements capable of translating G $beta$ $gamma$ binding into a functional effect. Whilst the I-II loop and/or the C-terminal may be the primary target for G $beta$ $gamma$ binding, the functional changes which occur upon binding, both in terms of a slowing of current activation kinetics and a reduction in current amplitude, are mediated by important molecular determinants within the $alpha$ 1B_1-483 sequence.

REFERENCES

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Abstract
Introduction
Methods
Results
Discussion
References

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[Abstract/Full Text] Herlitze, S., Garcia, D. E., Mackie, K., Hille, B., Scheuer, T. & Catterall, W. A. (1996). Modulation of Ca²⁺ channels by G-protein $beta$ $gamma$ subunits. Nature 380, 258-262.

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[Medline]

Acknowledgements

We thank the following for generous gifts of cDNAs: Dr T. Snutch (UBC, Canada) for $alpha$ 1E(rbEII) and $beta$ 1b; Dr H. Chin (NIH, USA) for $alpha$ 2- $delta$ ; Dr Y. Mori (Seiriken, Japan) for $alpha$ 1B; Dr E. Perez-Reyes (Loyola, USA) for $beta$ 2a; Dr M. Simon (CalTech, USA) for G $beta$ 1 and G $gamma$ 2; Professor P. G. Strange (Reading, UK) for D2 receptor; Dr T. Hughes (Yale University, USA) for Mut3-GFP; Genetics Institute (CA, USA) for pMT2. C. C. was a recipient of a postdoctoral fellowship from the Ministerio de Educacion y Ciencia of Spain. We also gratefully acknowledge financial support from The Wellcome Trust, and thank Ms A Odunlami, Mr I. Tedder, Ms M. Li and Ms J. May for technical assistance. This work benefited from the use of the Seqnet facility (Daresbury, UK).

Corresponding author

G. Stephens: Department of Pharmacology (Medawar), University College London, London WC1E 6BT, UK.

Email: g.stephens{at}ucl.ac.uk

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				J. L. Carroll, K. M. Boyle, M. J. Wasicko, and L. M. Sterni Dopamine D2 receptor modulation of carotid body type 1 cell intracellular calcium in developing rats Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L910 - L916. [Abstract] [Full Text] [PDF]

				A. Hummer, O. Delzeith, S. R. Gomez, R. L. Moreno, M. D. Mark, and S. Herlitze Competitive and Synergistic Interactions of G Protein {beta}2 and Ca2+ Channel {beta}1b Subunits with Cav2.1 Channels, Revealed by Mammalian Two-hybrid and Fluorescence Resonance Energy Transfer Measurements J. Biol. Chem., December 5, 2003; 278(49): 49386 - 49400. [Abstract] [Full Text] [PDF]

				A. C. Dolphin G Protein Modulation of Voltage-Gated Calcium Channels Pharmacol. Rev., December 1, 2003; 55(4): 607 - 627. [Abstract] [Full Text] [PDF]

				A. A. Simen, C. C. Lee, B. B. Simen, V. P. Bindokas, and R. J. Miller The C Terminus of the Ca Channel {alpha}1B Subunit Mediates Selective Inhibition by G-Protein-Coupled Receptors J. Neurosci., October 1, 2001; 21(19): 7587 - 7597. [Abstract] [Full Text] [PDF]

				D. C. Bell, A. J. Butcher, N. S. Berrow, K. M. Page, P. F. Brust, A. Nesterova, K. A. Stauderman, G. R. Seabrook, B. Nurnberg, and A. C. Dolphin Biophysical Properties, Pharmacology, and Modulation of Human, Neuronal L-Type ({alpha}1D, CaV1.3) Voltage-Dependent Calcium Currents J Neurophysiol, February 1, 2001; 85(2): 816 - 827. [Abstract] [Full Text] [PDF]

				A. A. Simen and R. J. Miller Involvement of Regions in Domain I in the Opioid Receptor Sensitivity of alpha 1B Ca2+ Channels Mol. Pharmacol., May 1, 2000; 57(5): 1064 - 1074. [Abstract] [Full Text]

				C. Canti, K. M. Page, G. J. Stephens, and A. C. Dolphin Identification of Residues in the N Terminus of alpha 1B Critical for Inhibition of the Voltage-Dependent Calcium Channel by Gbeta gamma J. Neurosci., August 15, 1999; 19(16): 6855 - 6864. [Abstract] [Full Text] [PDF]

				J. Hamid, D. Nelson, R. Spaetgens, S. J. Dubel, T. P. Snutch, and G. W. Zamponi Identification of an Integration Center for Cross-talk between Protein Kinase C and G Protein Modulation of N-type Calcium Channels J. Biol. Chem., March 5, 1999; 274(10): 6195 - 6202. [Abstract] [Full Text] [PDF]

				K. M. Page, C. Canti, G. J. Stephens, N. S. Berrow, and A. C. Dolphin Identification of the Amino Terminus of Neuronal Ca2+ Channel alpha 1 Subunits alpha 1B and alpha 1E as an Essential Determinant of G-Protein Modulation J. Neurosci., July 1, 1998; 18(13): 4815 - 4824. [Abstract] [Full Text] [PDF]

Role of domain I of neuronal Ca2+ channel 1 subunits in G protein modulation

Gary J. Stephens, Carles Cantí, Karen M. Page and Annette C. Dolphin

Role of domain I of neuronal Ca²⁺ channel $alpha$ 1 subunits in G protein modulation